【摘要】：目的觀察胃癌及其癌前病變中幽門螺旋桿菌(Helicobacter pylori,Hp)的感染和c-Jun、ATF-2蛋白表達(dá)情況,探討胃癌發(fā)生發(fā)展機(jī)制。方法采用免疫組織化學(xué)染色技術(shù)檢測(cè)淺表性胃炎(20例)、腸化(20例)、不典型增生(28例)、早期胃癌(36例)、進(jìn)展期胃癌(73例)中幽門螺桿菌(HP)感染和c-Jun、ATF-2蛋白的表達(dá)情況,用半定量評(píng)分方法評(píng)價(jià)各組c-Jun、ATF-2表達(dá),通過統(tǒng)計(jì)學(xué)分析探究其相互關(guān)系。結(jié)果免疫組化染色發(fā)現(xiàn),每組均有程度不同的Hp表達(dá),主要存在于胃小凹或者胃黏膜上皮細(xì)胞內(nèi),早期胃癌組及進(jìn)展期胃癌組Hp主要存在于癌灶周圍正常黏膜組織內(nèi),癌灶內(nèi)沒有Hp的表達(dá);統(tǒng)計(jì)學(xué)分析可見腸化組(30.00%)和不典型增生組(39.29%)Hp檢出率均明顯高于淺表性胃炎組(5.00%)、早期胃癌組(2.78%)和進(jìn)展期胃癌組(1.39%),差異有顯著性意義(P0.05)。c-Jun染色定位于細(xì)胞核,在淺表性胃炎組、腸化組可見少量c-Jun表達(dá),早期胃癌及進(jìn)展期胃癌組c-Jun表達(dá)率相對(duì)較高,早期胃癌組c-Jun表達(dá)率最高;不典型增生組(21.43%)、早期胃癌組(52.78%)、進(jìn)展期胃癌組(45.21%)c-Jun表達(dá)率均高于淺表性胃炎組(10.00%)(P0.05),早期胃癌組c-Jun表達(dá)率高于進(jìn)展期胃癌組(P0.05),胃癌組(47.71%)c-Jun表達(dá)率明顯高于非胃癌組(16.18%)(P0.05)。ATF-2在淺表性胃炎組未見陽性,其余4組均有多少不等的ATF-2表達(dá),染色均定位于細(xì)胞核;腸化組(10.00%)、不典型增生組ATF-2低表達(dá),早期胃癌組(38.90%)、進(jìn)展期胃癌組(41.10%)ATF-2高表達(dá),胃癌組(40.37%)ATF-2表達(dá)率高于非胃癌組(5.88%)(P0.05)。進(jìn)展期胃癌組中1例Hp感染陽性的病人c-Jun、ATF-2表達(dá)均顯示陽性。結(jié)論Hp感染與胃粘膜上皮腸上皮化生及不典型增生有關(guān);胃癌的形成過程中涉及c-Jun、ATF-2的高表達(dá)。
[Abstract]:Objective to observe the infection of Helicobacter pylori (Helicobacter pylori) HP and the expression of c-Junjiao ATF-2 protein in gastric cancer and its precancerous lesions, and to explore the mechanism of gastric cancer. Methods Immunohistochemical staining was used to detect the infection of Helicobacter pylori (HP) and the expression of c-Jun-ATF-2 protein in superficial gastritis (20 cases), intestinal metaplasia (20 cases), atypical hyperplasia (28 cases), early gastric cancer (36 cases) and advanced gastric cancer (73 cases). The expression of c Junan ATF-2 was evaluated by semi-quantitative scoring method and its relationship was studied by statistical analysis. Results Immunohistochemical staining showed that there were different levels of HP expression in each group, mainly in gastric fovea or gastric mucosal epithelium, while in early gastric cancer and advanced gastric cancer, HP mainly existed in the normal mucosa around the tumor. The positive rate of HP in intestinal metaplasia group (30.00%) and atypical hyperplasia group (39.29%) was significantly higher than that in superficial gastritis group (5.00%), early gastric cancer group (2.78%) and advanced gastric cancer group (1.39%). In superficial gastritis group, a small amount of c-Jun expression was found in intestinal metaplasia group. The expression rate of c-Jun was relatively high in early gastric cancer and advanced gastric cancer, and the highest in early gastric cancer group. The expression rate of c-Jun in atypical hyperplasia group (21.43%), early gastric cancer group (52.78%), advanced gastric cancer group (45.21%) was higher than that in superficial gastritis group (10.00%) (P0.05). The c-Jun expression rate in early gastric cancer group was higher than that in advanced gastric cancer group (P0.05), and the c-Jun expression rate in gastric cancer group (47.71%) was significantly higher than that in non-gastric cancer group (16.18%) (P0.05). No positive results were found in patients with sexual gastritis. The expression of ATF-2 in intestinal metaplasia group (10.00%), atypical hyperplasia group (38.90%), advanced gastric cancer group (41.10%), ATF-2 expression rate in gastric cancer group (40.37%) was higher than that in non-gastric cancer group (5.88%) (P0.05). In the advanced gastric cancer group, one patient with HP infection showed positive expression of c-Junjiao ATF-2. Conclusion HP infection is associated with intestinal metaplasia and atypical hyperplasia of gastric mucosa epithelium, and the high expression of c-Junan ATF-2 is involved in the formation of gastric carcinoma.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.2

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