發(fā)布時間：2018-07-02 09:21

  本文選題：小膠質細胞 + 巨噬細胞��； 參考：《第三軍醫(yī)大學學報》2017年11期

【摘要】：目的探討大鼠小膠質細胞及外周血巨噬細胞對膠質瘤C6細胞株遷移能力的影響。方法將大鼠小膠質細胞及外周血單核細胞誘導分化形成的巨噬細胞分別與膠質瘤C6細胞共培養(yǎng),以大鼠成纖維細胞共培養(yǎng)為陽性對照,單純膠質瘤C6細胞為空白對照,共培養(yǎng)0、24、48 h分別行劃痕實驗,于劃痕后6 h觀察劃痕實驗結果;共培養(yǎng)24、48 h時以CD11b標記、流式分選分離小膠質/巨噬細胞及C6細胞,行Western blot檢測0、24、48 h小膠質/巨噬細胞IL-10、IL-18和MMP-9及C6細胞株TGF-β、FAS配體表達水平的改變。結果早期小膠質細胞及巨噬細胞均抑制膠質瘤的遷移,巨噬細胞的抑制效果更強(P0.05);與膠質瘤細胞共培養(yǎng)后兩種細胞對腫瘤的抑制作用消失且差異無統(tǒng)計學意義,均表現為促進腫瘤遷移。小膠質細胞、巨噬細胞在初始階段IL-10、IL-18、金屬基質蛋白酶MMP-9表達略有不同,但共培養(yǎng)后兩種細胞的上述蛋白表達均升高且一致;共培養(yǎng)后的膠質瘤細胞的TGF-β、FAS配體較共培養(yǎng)前表達均升高且一致。結論小膠質細胞及巨噬細胞與C6膠質瘤細胞共培養(yǎng)后被馴化成同樣的GAMs,對C6細胞遷移影響的生物學表現一致。
[Abstract]:Objective to investigate the effects of microglia and peripheral blood macrophages on the migration of glioma C6 cells. Methods Rat microglial cells and macrophages derived from peripheral blood monocytes were co-cultured with glioma C6 cells respectively. Rat fibroblasts were co-cultured as positive control and glioma C6 cells as blank control. The results of scratch test were observed at 6 h after scratch, and CD11b labeled with CD11b was used to separate microglia / macrophages and C6 cells at 48 h after co-culture, and microglia / macrophages and C6 cells were separated by flow cytometry. The expression of IL-18 and MMP-9 in microglia / macrophages and the expression of TGF- 尾 -FAS ligand in C6 cell line were detected by Western blot. Results both microglia and macrophages inhibited the migration of gliomas in the early stage, and the inhibitory effect of macrophages was stronger (P0.05), but the inhibitory effects of the two kinds of cells on glioma disappeared after co-culture with glioma cells, and there was no significant difference between the two kinds of cells. All of them were found to promote tumor migration. The expression of IL-10 IL-18 and MMP-9 was slightly different in microglia and macrophage at the initial stage, but the expression of these proteins was increased and consistent after co-culture. The expression of TGF- 尾 -FAS ligand in co-cultured glioma cells was higher and consistent than that before co-culture. Conclusion microglia and macrophages co-cultured with C6 glioma cells were acclimated into the same GAMs.The biological effects on the migration of C6 cells were consistent.
【作者單位】： 第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所神經外科;
【基金】：國家自然科學基金(81172413)~~
【分類號】：R739.41

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