本文選題：miR-125b-1-3p + 胃癌�。� 參考：《蘭州大學》2017年碩士論文

【摘要】：目的:本研究通過探索miR-125b-1-3p在胃癌及對應癌旁組織中的表達情況,并分析其與胃癌臨床病理特征之間的關系,從而探討miR-125b-1-3p作為胃癌診斷標志物和治療靶點的可能性。方法:實驗前期利用基因芯片技術檢測14例早癌患者的胃癌組織及對應癌旁組織中miRNAs的表達譜分析情況。收集42例手術患者的胃癌組織及對應的癌旁組織。首先在胃癌樣本及癌旁樣本中提取總的RNA,運用實時熒光定量PCR檢測出miR-125b-1-3p在胃癌組織中表達水平,并分析miR-125b-1-3p的表達水平與胃癌臨床病理特征之間的關系。結果:實時熒光定量PCR結果提示胃癌組織miR-125b-1-3p的表達相較于對應癌旁組織存在顯著下調,有統(tǒng)計學差異。miR-125b-1-3p在胃癌的低表達僅與分化程度存在相關性,而與性別、TNM分期、浸潤深度、淋巴結轉移、年齡、腫瘤大小等胃癌臨床病理特征均無明顯相關性。在miR-125b-1-3p低表達的胃癌組織中,根據(jù)ROC曲線確定胃癌組織高/中分化程度的曲線下面積是0.714(P0.05),得出高/中分化程度的最佳界值:相對表達量為0.18,其在診斷高/中分化程度的敏感度70%、特異性72.2%、陽性預測值70%、陰性預測值30%。當相對表達量≥0.18時發(fā)生高/中分化程度的風險是相對表達量0.18時患者的2.33倍(P0.05),提示相對表達量≥0.18是胃癌組織高/中分化程度的獨立預測因素。結論:miR-125b-1-3p在胃癌組織中顯著下調,當miR-125b-1-3p的相對表達量≥0.18在預測胃癌高/中分化程度上有較高的診斷價值。
[Abstract]:Objective: to explore the expression of miR-125b-1-3p in gastric cancer and its relationship with the clinicopathological features of gastric cancer, and to explore the possibility of miR-125b-1-3p as a diagnostic marker and a therapeutic target for gastric cancer. Methods: the expression profiles of miRNAs in gastric cancer tissues and adjacent tissues of 14 patients with early cancer were detected by microarray technique. Gastric cancer tissues and corresponding paracancerous tissues were collected from 42 patients. First, the total RNAs were extracted from gastric cancer samples and adjacent samples. The expression of miR-125b-1-3p in gastric cancer tissues was detected by real-time fluorescent quantitative PCR, and the relationship between the expression level of miR-125b-1-3p and clinicopathological features of gastric cancer was analyzed. Results: the results of real-time fluorescence quantitative PCR showed that the expression of miR-125b-1-3p in gastric cancer was significantly down-regulated compared with the corresponding adjacent tissues, and the low expression of miR-125b-1-3p in gastric cancer was only correlated with the degree of differentiation, but with the TNM stage and depth of invasion. The clinicopathological features of gastric cancer, such as lymph node metastasis, age and tumor size, were not significantly correlated. In gastric cancer tissues with low expression of miR-125b-1-3p, According to the ROC curve, the area under the curve of high / medium differentiation degree of gastric cancer is 0.714 (P0.05). The best boundary value of high / medium differentiation degree is obtained: the relative expression is 0.18, its sensitivity in diagnosing high / medium differentiation degree is 70, specificity 72.2, positive. The predictive value is 70 and the negative predictive value is 30. The risk of high / moderate differentiation when relative expression 鈮，

本文編號：2087241