本文選題：SOX11核表達 + MCL�。� 參考：《南昌大學》2015年碩士論文

【摘要】：目的:研究探索SOXll核蛋白表達與套細胞淋巴瘤預后的關系。方法:1、總共收集到我院、江西省人民醫(yī)院2002年1月至2011年12月收治的102例MCL患者資料。2、最終符合入組標準者48例;男性30例,女性18例。3、采集48例MCL患者病理組織切片,經烤片、脫蠟、水化等處理后,滴加入S0X11單克隆抗體(American Santa Cruz)。4、顯微鏡下觀察MCL腫瘤細胞S0X11核表達,細胞核可見到棕黃色細顆粒者是陽性染色(部分切片合并細胞質染色陽性),腫瘤細胞未出現陽性染色則是陰性。5、每張陽性切片隨機選取5個視野(400倍),計算出每個視野陽性細胞百分比,5個百分比的平均值為該病例S0X11核表達率。6、結合相關文獻,參考十三點評分法,將有陽性細胞的切片判定為S0X11核表達陽性,沒有陽性細胞的切片判定為S0X11核表達陰性。7、參考十三點評分法,將病例分為陰性組(S0X11核表達陰性),弱陽性組(S0X11核表達陽性率50%),陽性組(50%≤S0X11核表達陽性率80%),強陽性組(S0X11核表達陽性率≥80%)。8、對48例MCL病例進行隨訪,明確每例病例的總生存期(OS)。9、應用Cox回歸分析,對陰性、弱陽性、陽性、強陽性四組病例生存時間進行分析,先后使用了分類變量、連續(xù)變量兩種方式分析處理。結果:1、弱陽性組、陽性組、強陽性組與陰性組比較生存時間沒有顯著性差異(P0.05)。2、隨著SOXII核表達陽性率增加,死亡危險顯著性增加(P0.05)。結論:MCL患者SOX11核蛋白表達水平與預后呈負相關,SOX11核蛋白表達率越高預后越差。
[Abstract]:Objective: to investigate the relationship between SOXll nuclear protein expression and prognosis of mantle cell lymphoma. Methods A total of 102 cases of MCL patients admitted to Jiangxi Provincial people's Hospital from January 2002 to December 2011 were collected. Data of 102 patients with MCL were collected from January 2002 to December 2011. Finally, 48 patients with MCL were included in the study, including 30 males, 18 females, and 48 patients with MCL. After treated with roasted slice, dewaxing and hydration, S0X11 monoclonal antibody (American Santa Cruz). 4 was added. The nuclear expression of S0X11 was observed under microscope. The cells with fine brown granules were positive (some sections were positive with cytoplasm staining), but the tumor cells were negative. 5 fields (400 times) were randomly selected for each positive section. The average of 5 percent of positive cells was S0X11 nuclear expression rate. Referring to the 13:00 scoring method, the sections with positive cells were determined to be positive for S0X11 nuclear expression, while sections without positive cells were determined to be negative for S0X11 nuclear expression. The patients were divided into negative group (negative expression of S0X11 nucleus), weak positive group (50% of S0X11 nuclear expression), positive group (50% 鈮，

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