本文選題：SOX11核表達(dá) + MCL��； 參考：《南昌大學(xué)》2015年碩士論文

【摘要】：目的:研究探索SOXll核蛋白表達(dá)與套細(xì)胞淋巴瘤預(yù)后的關(guān)系。方法:1、總共收集到我院、江西省人民醫(yī)院2002年1月至2011年12月收治的102例MCL患者資料。2、最終符合入組標(biāo)準(zhǔn)者48例;男性30例,女性18例。3、采集48例MCL患者病理組織切片,經(jīng)烤片、脫蠟、水化等處理后,滴加入S0X11單克隆抗體(American Santa Cruz)。4、顯微鏡下觀察MCL腫瘤細(xì)胞S0X11核表達(dá),細(xì)胞核可見到棕黃色細(xì)顆粒者是陽性染色(部分切片合并細(xì)胞質(zhì)染色陽性),腫瘤細(xì)胞未出現(xiàn)陽性染色則是陰性。5、每張陽性切片隨機(jī)選取5個視野(400倍),計算出每個視野陽性細(xì)胞百分比,5個百分比的平均值為該病例S0X11核表達(dá)率。6、結(jié)合相關(guān)文獻(xiàn),參考十三點(diǎn)評分法,將有陽性細(xì)胞的切片判定為S0X11核表達(dá)陽性,沒有陽性細(xì)胞的切片判定為S0X11核表達(dá)陰性。7、參考十三點(diǎn)評分法,將病例分為陰性組(S0X11核表達(dá)陰性),弱陽性組(S0X11核表達(dá)陽性率50%),陽性組(50%≤S0X11核表達(dá)陽性率80%),強(qiáng)陽性組(S0X11核表達(dá)陽性率≥80%)。8、對48例MCL病例進(jìn)行隨訪,明確每例病例的總生存期(OS)。9、應(yīng)用Cox回歸分析,對陰性、弱陽性、陽性、強(qiáng)陽性四組病例生存時間進(jìn)行分析,先后使用了分類變量、連續(xù)變量兩種方式分析處理。結(jié)果:1、弱陽性組、陽性組、強(qiáng)陽性組與陰性組比較生存時間沒有顯著性差異(P0.05)。2、隨著SOXII核表達(dá)陽性率增加,死亡危險顯著性增加(P0.05)。結(jié)論:MCL患者SOX11核蛋白表達(dá)水平與預(yù)后呈負(fù)相關(guān),SOX11核蛋白表達(dá)率越高預(yù)后越差。
[Abstract]:Objective: to investigate the relationship between SOXll nuclear protein expression and prognosis of mantle cell lymphoma. Methods A total of 102 cases of MCL patients admitted to Jiangxi Provincial people's Hospital from January 2002 to December 2011 were collected. Data of 102 patients with MCL were collected from January 2002 to December 2011. Finally, 48 patients with MCL were included in the study, including 30 males, 18 females, and 48 patients with MCL. After treated with roasted slice, dewaxing and hydration, S0X11 monoclonal antibody (American Santa Cruz). 4 was added. The nuclear expression of S0X11 was observed under microscope. The cells with fine brown granules were positive (some sections were positive with cytoplasm staining), but the tumor cells were negative. 5 fields (400 times) were randomly selected for each positive section. The average of 5 percent of positive cells was S0X11 nuclear expression rate. Referring to the 13:00 scoring method, the sections with positive cells were determined to be positive for S0X11 nuclear expression, while sections without positive cells were determined to be negative for S0X11 nuclear expression. The patients were divided into negative group (negative expression of S0X11 nucleus), weak positive group (50% of S0X11 nuclear expression), positive group (50% 鈮，

本文編號：2064701