本文選題：TET + 乳腺癌�。� 參考：《腫瘤防治研究》2017年07期

【摘要】：目的探討TET1對乳腺癌MDA-MB-231細胞增殖、轉移、侵襲等生物學行為的影響,并初步探究其相關分子機制。方法應用慢病毒載體建立TET1過表達穩(wěn)轉細胞株(MDA-MB-231-TET1)及陰性對照組穩(wěn)轉細胞株(MDA-MB-231-NC),Real-time PCR及Western blot法檢測TET1的表達情況。CCK-8法、流式細胞術檢測細胞的增殖及細胞周期分布,細胞劃痕實驗及Transwell小室檢測細胞遷移及侵襲能力;Western blot及免疫熒光實驗檢測EMT相關蛋白(E-cadherin、N-cadherin、Vimentin、β-catenin)分子的表達。結果成功構建過表達TET1細胞株(P=0.03)。相較于陰性對照組和空白對照組細胞,MDA-MB-231-TET1組細胞增殖、遷移及侵襲明顯受到抑制(P0.001),G2/M期細胞周期阻滯(P=0.002);同時,伴隨E-cadherin表達升高(P0.001),N-cadherin(P=0.003)、Vimentin(P=0.041)表達降低,β-catenin(P0.001)表達降低,并且TET1可抑制β-catenin向細胞核內轉移,進而抑制EMT的發(fā)生。結論 TET1可抑制MDA-MB-231細胞增殖,并能抑制其遷移及侵襲,其機制可能與通過Wnt/β-catenin通路抑制EMT的發(fā)生相關。
[Abstract]:Objective to investigate the effects of TET1 on proliferation, metastasis and invasion of breast cancer MDA-MB-231 cells, and to explore its molecular mechanism. Methods the expression of TET1 was detected by real-time PCR and Western blot, and the proliferation and cell cycle distribution were detected by flow cytometry (FCM), and the expression of Tet 1 was detected by real-time PCR and Western blot assay respectively by using lentivirus vector and MDA-MB-231-TET1 and MDA-MB-231-NC, respectively. The expression of E-cadherin N-cadherin (尾 -catenin) was detected by cell scratch assay, transwell chamber assay, and the expression of E-cadherin N-cadherin (尾 -catenin) by Western blot. Results TET1 cell line was successfully constructed (P0. 03). The cell proliferation, migration and invasion of MDA-MB-231-TET1 cells were significantly inhibited (P0.001) in G _ 2 / M phase cell cycle arrest (P _ (0.002), and the expression of 尾 -catenin (P _ (0.001) decreased with the increase of E-cadherin expression (P _ (0.001), N-cadherin (P _ (0.003) Vimentin (P _ (0.041) expression decreased, and the expression of 尾 -catenin (P _ (0.001) decreased in MDA-MB-231-TET1 group. Moreover, TET1 inhibited 尾-catenin transfer to the nucleus, which inhibited the occurrence of EMT. Conclusion TET1 can inhibit the proliferation, migration and invasion of MDA-MB-231 cells, and its mechanism may be related to the inhibition of EMT through Wnt- 尾 -catenin pathway.
【作者單位】： 河北醫(yī)科大學第四醫(yī)院乳腺中心;
【基金】：河北省自然科學基金(H2015206466)
【分類號】：R737.9

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