本文選題：TET + 乳腺癌　； 參考：《腫瘤防治研究》2017年07期

【摘要】：目的探討TET1對(duì)乳腺癌MDA-MB-231細(xì)胞增殖、轉(zhuǎn)移、侵襲等生物學(xué)行為的影響,并初步探究其相關(guān)分子機(jī)制。方法應(yīng)用慢病毒載體建立TET1過(guò)表達(dá)穩(wěn)轉(zhuǎn)細(xì)胞株(MDA-MB-231-TET1)及陰性對(duì)照組穩(wěn)轉(zhuǎn)細(xì)胞株(MDA-MB-231-NC),Real-time PCR及Western blot法檢測(cè)TET1的表達(dá)情況。CCK-8法、流式細(xì)胞術(shù)檢測(cè)細(xì)胞的增殖及細(xì)胞周期分布,細(xì)胞劃痕實(shí)驗(yàn)及Transwell小室檢測(cè)細(xì)胞遷移及侵襲能力;Western blot及免疫熒光實(shí)驗(yàn)檢測(cè)EMT相關(guān)蛋白(E-cadherin、N-cadherin、Vimentin、β-catenin)分子的表達(dá)。結(jié)果成功構(gòu)建過(guò)表達(dá)TET1細(xì)胞株(P=0.03)。相較于陰性對(duì)照組和空白對(duì)照組細(xì)胞,MDA-MB-231-TET1組細(xì)胞增殖、遷移及侵襲明顯受到抑制(P0.001),G2/M期細(xì)胞周期阻滯(P=0.002);同時(shí),伴隨E-cadherin表達(dá)升高(P0.001),N-cadherin(P=0.003)、Vimentin(P=0.041)表達(dá)降低,β-catenin(P0.001)表達(dá)降低,并且TET1可抑制β-catenin向細(xì)胞核內(nèi)轉(zhuǎn)移,進(jìn)而抑制EMT的發(fā)生。結(jié)論 TET1可抑制MDA-MB-231細(xì)胞增殖,并能抑制其遷移及侵襲,其機(jī)制可能與通過(guò)Wnt/β-catenin通路抑制EMT的發(fā)生相關(guān)。
[Abstract]:Objective to investigate the effects of TET1 on proliferation, metastasis and invasion of breast cancer MDA-MB-231 cells, and to explore its molecular mechanism. Methods the expression of TET1 was detected by real-time PCR and Western blot, and the proliferation and cell cycle distribution were detected by flow cytometry (FCM), and the expression of Tet 1 was detected by real-time PCR and Western blot assay respectively by using lentivirus vector and MDA-MB-231-TET1 and MDA-MB-231-NC, respectively. The expression of E-cadherin N-cadherin (尾 -catenin) was detected by cell scratch assay, transwell chamber assay, and the expression of E-cadherin N-cadherin (尾 -catenin) by Western blot. Results TET1 cell line was successfully constructed (P0. 03). The cell proliferation, migration and invasion of MDA-MB-231-TET1 cells were significantly inhibited (P0.001) in G _ 2 / M phase cell cycle arrest (P _ (0.002), and the expression of 尾 -catenin (P _ (0.001) decreased with the increase of E-cadherin expression (P _ (0.001), N-cadherin (P _ (0.003) Vimentin (P _ (0.041) expression decreased, and the expression of 尾 -catenin (P _ (0.001) decreased in MDA-MB-231-TET1 group. Moreover, TET1 inhibited 尾-catenin transfer to the nucleus, which inhibited the occurrence of EMT. Conclusion TET1 can inhibit the proliferation, migration and invasion of MDA-MB-231 cells, and its mechanism may be related to the inhibition of EMT through Wnt- 尾 -catenin pathway.
【作者單位】： 河北醫(yī)科大學(xué)第四醫(yī)院乳腺中心;
【基金】：河北省自然科學(xué)基金(H2015206466)
【分類(lèi)號(hào)】：R737.9

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