本文選題：多發(fā)性骨髓瘤 + 熒光原位雜交��； 參考：《中國實驗血液學(xué)雜志》2017年03期

【摘要】：目的:比較直接熒光原位雜交技術(shù)(D-FISH)和CD-138磁珠分選結(jié)合FISH(MACS-FISH)的方法檢測多發(fā)性骨髓瘤(MM)的細(xì)胞遺傳學(xué)異常。方法:對31例MM患者進(jìn)行了傳統(tǒng)G顯帶核型分析,并采用探針組合(1q21,D13S319,RB1,IgH,P53)同時進(jìn)行D-FISH法和MACS-FISH法的檢測。對17例IgH重組異常的患者,進(jìn)一步利用IgH/FGFR3,IgH/MAF,IgH/CCND1 3種探針進(jìn)行FISH檢測。結(jié)果:31例患者中5例(16.1%)核型分析具有異�？寺�。采用直接FISH法有13例(41.9%)檢出異常,而采用CD138磁珠分選漿細(xì)胞后有25例(80.6%)檢出異常。二者異常檢出率有顯著差異(P=0.042)。采用D-FISH法,1q21,D13S319,RB1,IgH,P53 5種探針的異常檢出率分別為22.6%,25.8%,29%,38.7%和9.7%;而采用MACS-FISH法上述5種探針異常檢出率分別為48.4%,45.2%,48.4%,67.7%和16.1%。骨髓漿細(xì)胞比例≥20%時,D-FISH與MACS-FISH異常檢出率一致;骨髓漿細(xì)胞比例20%,MACS-FISH異常檢出率明顯高于D-FISH法,二者有統(tǒng)計學(xué)差異(P=0.00)。結(jié)論:利用CD-138磁珠分選后進(jìn)行FISH檢測能顯著提高M(jìn)M細(xì)胞遺傳學(xué)異常的檢出率。常規(guī)核型分析結(jié)合MACS-FISH是MM細(xì)胞遺傳學(xué)異�？寺z測的理想方法,尤其適用于骨髓漿細(xì)胞比例小于20%的患者。
[Abstract]:Objective: to compare the methods of direct fluorescence in situ hybridization (D-FISH) and CD-138 magnetic bead sorting combined with FISHS-FISH to detect the cytogenetic abnormalities of multiple myeloma. Methods: the conventional G-banding karyotype analysis was performed in 31 patients with MM, and D-FISH and MACS-FISH methods were used to detect the karyotype of 31 patients with MM. In 17 patients with abnormal igh recombination, fish was detected by using IgH / FGFR3 / IgH / MAFN IgH / CCND1 probes. Results the karyotype analysis of 5 out of 31 cases was abnormal clone. The abnormality was detected in 13 cases by direct fish method and in 25 cases by CD138 magnetic bead sorting. There was a significant difference in abnormal detection rate between the two groups. The detectable rate of the five probes with D-FISH method was 22.60.25.8% and 9.7%, respectively, while that of MACS-FISH was 48.45.28.48.47.7% and 16.1%, respectively. The detection rate of D-FISH and MACS-FISH was the same when the ratio of bone marrow plasma cells was 鈮，

本文編號：2047436