本文選題：胃癌 + 微小RNA-��； 參考：《中國病理生理雜志》2017年07期

【摘要】：目的:研究微小RNA-9(microRNA-9,miR-9)對胃癌SGC-7901細(xì)胞上皮-間充質(zhì)轉(zhuǎn)化(EMT)功能的影響及其相關(guān)機(jī)制。方法:SGC-7901胃癌細(xì)胞株分別轉(zhuǎn)染miR-9 mimics和陰性對照序列(negative control mimic,NCM),作為miR-9組和NCM組,并設(shè)立未轉(zhuǎn)染對照(control)組,采用RT-qPCR法檢測各組細(xì)胞miR-9的含量,Transwell實驗檢測3組細(xì)胞遷移能力和侵襲能力,Western blot法檢測3組細(xì)胞的N-cadherin、E-cadherin、α-catenin和神經(jīng)纖毛蛋白1(NRP1)表達(dá)水平。采用Western blot法檢測NRP1過表達(dá)對miR-9抑制EMT的拮抗作用。雙螢光素酶實驗檢測miR-9與NRP1的關(guān)系。結(jié)果:miR-9組的miR-9表達(dá)水平明顯上調(diào),為control組的538倍(P0.05)。miR-9組的遷移細(xì)胞數(shù)量明顯低于control組(P0.05)。miR-9組的侵襲細(xì)胞數(shù)量明顯低于control組(P0.05)。miR-9組細(xì)胞的N-cadherin和NRP1蛋白表達(dá)量明顯降低,E-cadherin及α-catenin蛋白表達(dá)量明顯升高。而NRP1及miR-9均過表達(dá)組胃癌細(xì)胞中N-cadherin蛋白表達(dá)量明顯升高,E-cadherin及α-catenin蛋白表達(dá)量明顯降低。雙螢光素酶檢驗結(jié)果顯示NRP1為miR-9的下游靶基因(P0.05)。結(jié)論:miR-9可能通過降低下游靶基因NRP1水平影響EMT相關(guān)蛋白表達(dá),抑制胃癌SGC-7901細(xì)胞的EMT功能。
[Abstract]:Aim: to study the effect of microRNA-9 miR-9 on the epithelial-mesenchymal transformation (EMTT) function of gastric cancer SGC-7901 cells and its related mechanism. Methods: human SGC-7901 gastric cancer cell line was transfected with miR-9 mimics and negative control mimicia NCMI respectively as miR-9 group and NCM-negative control group. The content of miR-9 was detected by RT-qPCR and the expression levels of N-cadherin-E-cadherin, 偽 -catenin and neurociliated protein 1nRP1 were detected by Transwell assay and Western blot assay. Western blot assay was used to detect the antagonistic effect of NRP1 overexpression on the inhibition of EMT by miR-9. The relationship between miR-9 and NRP1 was detected by double luciferase assay. Results the miR-9 expression level was significantly up-regulated in the miR-9 group. The number of migration cells in control group was significantly lower than that in control group. The number of invasive cells was significantly lower in control group than that in control group. The expression of N-cadherin and NRP1 protein in control group was significantly lower than that in control group and the expression of E-cadherin and 偽 -catenin protein was significantly increased. The expression of N-cadherin and 偽 -catenin in gastric cancer cells were significantly increased in both NRP1 and miR-9 overexpression groups, and the expression of E-cadherin and 偽 -catenin were significantly decreased. The results of double luciferase assay showed that NRP1 was the downstream target gene of miR-9. Conclusion the expression of EMT related protein may be affected by reducing the level of downstream target gene NRP1 and inhibiting the EMT function of gastric cancer SGC-7901 cells.
【作者單位】： 天津市第五中心醫(yī)院消化科;
【基金】：濱海新區(qū)衛(wèi)生局基金資助項目(No.2013BWKY031)
【分類號】：R735.2

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本文編號：2044212