本文選題：不飽和脂肪酸 + 人胃癌細(xì)胞　； 參考：《中國臨床藥理學(xué)雜志》2017年10期

【摘要】：目的研究不飽和脂肪酸對人胃癌細(xì)胞蛋白激酶B(PKB/Akt)信號通路的調(diào)控作用。方法取胃癌患者腫瘤組織,移植到重癥聯(lián)合免疫缺陷(SCID)小鼠上,將移植瘤組織酶解消化,分離出細(xì)胞進(jìn)行傳代培養(yǎng)。然后將傳代細(xì)胞分為5組:低中高3個濃度實驗組(不飽和脂肪酸0.3,0.6,1.2 mg·L~(-1)),對照組(5-氟尿嘧啶10 mg·L~(-1)),溶劑對照組(二甲基亞砜30μL·L~(-1)),給藥后培養(yǎng)48 h。用噻唑藍(lán)(MTT)法檢測不飽和脂肪酸對人胃癌細(xì)胞的增殖抑制作用,用酶聯(lián)免疫吸附法檢測胃癌細(xì)胞中p21、雙微體基因(MDM2)及PKB/Akt表達(dá)水平。結(jié)果高中低3個濃度實驗組人胃癌細(xì)胞增殖抑制率比對照組分別升高了31.7%,55.51%,68.28%;這3組的p21和MDM2水平比對照組分別升高了4.39%,80.12%,161.36%和19.81%,97.76%,207.35%;這3組的PKB/Akt水平比對照組降低了13.31%,57.69%,78.71%。結(jié)論不飽和脂肪酸對人胃癌細(xì)胞增殖的抑制作用主要與升高腫瘤細(xì)胞內(nèi)p21與MDM2水平和抑制PKB/Akt信號通路的活性有關(guān)。
[Abstract]:Objective to investigate the regulatory effect of unsaturated fatty acids on protein kinase BPKB / Akt signaling pathway in human gastric cancer cells. Methods the tumor tissues of gastric cancer patients were transplanted to mice with severe combined immunodeficiency syndrome (SCID). The transplanted tumor tissues were digested by enzyme and the cells were isolated and cultured. Then the passage cells were divided into five groups: low, medium and high concentration experimental group (unsaturated fatty acid 0.3U 0.61.2mg / L), control group (10 mg / L 5-fluorouracil) and solvent control group (30 渭 L / L DMSO) cultured for 48 h. The inhibitory effect of unsaturated fatty acids on the proliferation of human gastric cancer cells was detected by thiazolyl methacrylate (MTT) assay. The expression levels of p21, MDM2 and PKB / Akt in gastric cancer cells were detected by enzyme-linked immunosorbent assay (Elisa). Results compared with the control group, the inhibition rate of proliferation of gastric cancer cells in the three experimental groups increased 31.75.51 and 68.28.The levels of p21 and MDM2 in the three groups were 4.3980.120.12and 97.767.36% higher than those in the control group, respectively, and the PKB / Akt level in these three groups was 13.31% lower than that in the control group (78.71%). Conclusion the inhibitory effect of unsaturated fatty acids on the proliferation of human gastric cancer cells is related to the increase of p21 and MDM2 levels and the inhibition of PKB / Akt signaling pathway activity.
【作者單位】： 甘肅政法學(xué)院甘肅省證據(jù)科學(xué)技術(shù)研究與應(yīng)用重點實驗室;
【基金】：甘肅省基礎(chǔ)研究創(chuàng)新群體基金資助項目(145RJIA333) 蘭州市科技計劃基金資助項目(2014-1-182;2015-3-80) 甘肅省高校科技創(chuàng)新團(tuán)隊基金資助項目(2016C-09) 蘭州市人才創(chuàng)新創(chuàng)業(yè)基金資助項目(2016-RC-2) 西北民族地區(qū)偵查理論與實務(wù)研究中心基金資助項目 甘肅政法學(xué)院重大項目基金資助項目(2016XZD15)
【分類號】：R979.1

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相關(guān)期刊論文 前6條

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