本文選題：BDNF + Ets-1�。� 參考：《青島大學》2017年碩士論文

【摘要】：目的:該研究主要是進一步論證與卵巢癌侵襲轉移相關的BDNF、Ets-1這兩個基因蛋白在上皮性漿液性卵巢癌的表達情況與臨床病理特征之間的關系及兩者之間的相關性。研究BDNF、Ets-1在上皮性漿液性卵巢癌的發(fā)生及發(fā)展關系。方法:通過免疫組化及Western Blot方法檢測BDNF、Ets-1這兩個基因蛋白在上皮性漿液性卵巢癌石蠟切片及新鮮卵巢組織中的表達情況,分析BDNF、Ets-1與臨床病理特征(臨床分期、分化程度、淋巴結轉移、遠處轉移、年齡等)的關系,及進一步研究兩種基因蛋白在上皮性漿液性卵巢癌中表達的相關性。結果:1.BDNF在正常卵巢組織中的的陽性表達率為15%,在交界性卵巢組織中陽性表達率為40%,在上皮性漿液性卵巢癌的陽性表達率為67%,主要位于細胞質中,由此可見在惡性卵巢組織中的陽性表達率最高,差異有統(tǒng)計學意義(P0.05)。2.BDNF在漿液性卵巢癌中的表達與臨床分期(P=0.001)、有無遠處轉移(P=0.004)、有無淋巴結轉移(P=0.000)及組織分化程度(P=0.044)有關,差異有統(tǒng)計學意義,(P0.05),與年齡無明顯關系(P0.05)。BDNF在臨床分期晚期、有遠處轉移、有淋巴結轉移、組織分化程度低組中呈高表達。BDNF在臨床分期早期、無遠處轉移、無淋巴結轉移、組織分化程度高組中表達水平低。3.Ets-1在卵巢良性組織中陽性表達率為22%,在交界性卵巢組織中陽性表達率為44%,在上皮性漿液性卵巢癌組織中陽性表達率為50%,主要位于細胞間質中,少量位于細胞核中�？梢钥闯�,Ets-1在三種不同卵巢組織中表達情況存在明顯差距,三組之間X2=2.0,P=0.000,差異有統(tǒng)計學意義(P0.05)。4.Ets-1在漿液性卵巢癌中的表達與臨床分期(P=0.024)、有無遠處轉移(P=0.000)、有無淋巴結轉移(P=0.003)及組織分化程度(P=0.001)有關,差異有統(tǒng)計學意義,(P0.005),與年齡無明顯關系(P0.05)。Ets-1在臨床分期晚期、有遠處轉移、有淋巴結轉移、組織分化程度低組中呈高表達。Ets-1在臨床分期早期、無遠處轉移、無淋巴結轉移、組織分化程度高組中表達水平低或幾乎無表達。5.經過Spearman相關性分析,在上皮性漿液性卵巢癌組織中BDNF表達強度增加,Ets-1表達強度也增加,且隨著卵巢癌惡性程度的增加,BDNF、Ets-1兩個基因蛋白表達也增加,P=0.041,兩者在上皮性漿液性卵巢癌組織的表達成正相關(r=0.447,P0.05)。結論:1.BDNF、Ets-1這兩個基因蛋白在上皮性漿液性卵巢癌組織中高表達,且隨著惡性程度的增加,表達水平也升高,說明BDNF、Ets-1可能參與了卵巢癌的發(fā)生及發(fā)展過程。2.BDNF、Ets-1表達水平與卵巢癌患者的預后因素有關,說明BDNF、Ets-1可能影響患者的預后。抑制這兩個基因蛋白的表達將來可能成為治療卵巢癌的一個重要的治療方法。3.經過Spearman相關性分析,在上皮性漿液性卵巢癌組織中BDNF表達強度增加,Ets-1表達強度也增加,且隨著卵巢癌惡性程度的增加,BDNF、Ets-1兩個基因蛋白表達也增加,兩者在上皮性漿液性卵巢癌組織的表達成正相關。
[Abstract]:Objective: This study is to further demonstrate the relationship between the expression of BDNF, Ets-1 and the clinicopathological features of the two gene proteins associated with the invasion and metastasis of ovarian cancer, and the relationship between them and the relationship between them. The relationship between the development and development of BDNF and Ets-1 in epithelial serous ovarian cancer is studied. Methods: Immunohistochemical and Western Blot methods were used to detect the expression of BDNF, Ets-1, two gene proteins in the paraffin and fresh ovarian tissues of epithelial serous ovarian cancer, and to analyze the relationship between BDNF, Ets-1 and clinicopathological features (clinical stage, differentiation, lymph node metastasis, distant migration, age, etc.), and further study the two kinds of gene eggs. The positive expression rate of 1.BDNF in normal ovarian tissue was 15%, the positive expression rate in the borderline ovarian tissue was 40%, the positive expression rate in epithelial serous ovarian cancer was 67%, mainly in the cytoplasm, thus the positive form in the malignant ovarian tissue was found. The difference was statistically significant (P0.05), the expression of.2.BDNF in serous ovarian cancer and clinical stage (P=0.001), distant metastasis (P=0.004), lymph node metastasis (P=0.000) and the degree of tissue differentiation (P=0.044), the difference was statistically significant, (P0.05), and there was no significant relationship with age (P0.05).BDNF in the late stage of clinical stage. The positive expression rate of.BDNF in the benign tissue of the ovary was 22%, the positive rate in the borderline ovarian tissue was 44%, and the positive expression rate in the borderline ovarian tissue was 44%, in the epithelial serous sex. The positive expression rate in ovarian cancer tissues was 50%, mainly in the cytoplasm and a small amount in the nucleus. It can be seen that the expression of Ets-1 in three different ovarian tissues has a significant difference, and the difference between the three groups is X2=2.0 and P=0.000, the difference is statistically significant (P0.05), the expression of.4.Ets-1 in the serous ovarian cancer and the clinical stage (P=0.024). No distant metastasis (P=0.000) was associated with lymph node metastasis (P=0.003) and the degree of tissue differentiation (P=0.001). The difference was statistically significant, (P0.005), and there was no significant relationship with age (P0.05).Ets-1 in the late stage of clinical stage, with distant metastasis, lymph node metastasis, and high expression of.Ets-1 in the low level of tissue differentiation in the early stage of clinical staging and no distant transition. The expression of BDNF in the epithelial serous ovarian cancer tissues increased and the expression of Ets-1 increased, and the expression of Ets-1 was increased with the increase of the malignant degree of ovarian cancer, and the expression of two gene proteins in BDNF and Ets-1 increased with the increase of the malignant degree of ovarian cancer, and the expression of two gene proteins in BDNF and Ets-1 also increased, P=0.041, The expression of the two in epithelial serous ovarian cancer tissue is positive correlation (r=0.447, P0.05). Conclusion: 1.BDNF, Ets-1 these two gene proteins are highly expressed in epithelial serous ovarian cancer tissue, and with the increase of the malignant degree, the expression level is also elevated, indicating that BDNF, Ets-1 may be involved in the development and development of ovarian cancer.2.BDNF, Ets-1 Expression level is associated with prognostic factors in ovarian cancer patients, indicating that BDNF, Ets-1 may affect the prognosis of patients. Inhibition of the expression of these two gene proteins may become an important treatment for ovarian cancer in the future,.3. after Spearman correlation analysis, the increase of BDNF expression in epithelial serous ovarian cancer, Ets-1 table With the increase of the malignant degree of ovarian cancer, the expression of the two gene proteins of BDNF and Ets-1 also increased, which was positively related to the expression of epithelial serous ovarian cancer.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.31

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