本文選題：胃癌 + NLRC　； 參考：《安徽醫(yī)科大學(xué)學(xué)報(bào)》2017年10期

【摘要】：目的觀察核苷酸結(jié)合結(jié)構(gòu)域受體家族含CARD結(jié)構(gòu)域-5(NLRC5)、β-catenin在胃癌組織中的表達(dá)情況。分析NLRC5與胃癌臨床病理特征及預(yù)后的關(guān)系。并初步探討NLRC5與Wnt/β-catenin信號(hào)通路間的關(guān)系。方法收集根治性胃癌切除術(shù)患者石蠟標(biāo)本93例,免疫組化檢測(cè)胃癌組織中NLRC5和β-catenin的表達(dá)情況;分析NLRC5的表達(dá)與胃癌臨床病理特征的關(guān)系。采用胃癌細(xì)胞株MKN-45和MGC-803分別轉(zhuǎn)染p EGFP-C2-NLRC5重組質(zhì)粒,qRT-PCR法和Western blot法驗(yàn)證NLRC5轉(zhuǎn)染效率。采用MTT細(xì)胞增殖和細(xì)胞劃痕實(shí)驗(yàn)對(duì)NLRC5進(jìn)行功能分析,觀察體外實(shí)驗(yàn)中NLRC5表達(dá)變化對(duì)胃癌細(xì)胞的生物學(xué)行為的影響,采用Western blot法檢測(cè)Wnt/β-catenin信號(hào)通路相關(guān)蛋白的變化。結(jié)果 NLRC5在93例胃癌標(biāo)本中陽(yáng)性表達(dá)67例(72.04%),陰性表達(dá)26例(27.96%)。NLCR5的表達(dá)情況與胃癌的TNM分期、淋巴結(jié)轉(zhuǎn)移和復(fù)發(fā)情況密切相關(guān)。Kaplan-merier生存分析結(jié)果顯示NLCR5陰性表達(dá)的患者預(yù)后好于陽(yáng)性表達(dá)患者(P0.05)。93例胃癌標(biāo)本中β-catenin陽(yáng)性表達(dá)63例(67.74%),陰性表達(dá)30例(32.26%)。Cox多因素回歸分析顯示,NLRC5和β-catenin的陽(yáng)性表達(dá)、低TNM分期、淋巴結(jié)轉(zhuǎn)移是影響胃癌患者預(yù)后的獨(dú)立危險(xiǎn)因素。胃癌細(xì)胞在過(guò)表達(dá)NLRC5后,MTT細(xì)胞增殖和劃痕實(shí)驗(yàn)結(jié)果顯示,細(xì)胞的增殖和遷移能力顯著增強(qiáng)(P0.05,P0.01),Western blot實(shí)驗(yàn)結(jié)果顯示,Wnt/β-catenin信號(hào)通路中關(guān)鍵蛋白β-catenin和下游關(guān)鍵靶蛋白C-myc、金屬基質(zhì)蛋白酶-7的表達(dá)水平明顯提高。結(jié)論 NLRC5廣泛表達(dá)于胃癌組織中,其表達(dá)與胃癌的TNM分期和淋巴結(jié)轉(zhuǎn)移密切相關(guān)。Kaplan-merier生存分析顯示:NLRC5高表達(dá)是影響胃癌預(yù)后的危險(xiǎn)因素。Cox多因素回歸分析顯示:NLRC5高表達(dá)是影響胃癌預(yù)后的獨(dú)立危險(xiǎn)因素。NLRC5與β-catenin的表達(dá)呈正相關(guān)性,NLRC5的表達(dá)變化影響胃癌細(xì)胞的生物學(xué)行為和Wnt/β-catenin信號(hào)通路的關(guān)鍵蛋白的表達(dá)。提示NLRC5可能通過(guò)調(diào)控Wnt/β-catenin信號(hào)通路影響胃癌的浸潤(rùn)和轉(zhuǎn)移。
[Abstract]:Objective to investigate the expression of nucleotide binding domain receptor family (NLRC5, 尾 -catenin) in gastric carcinoma. To analyze the relationship between NLRC 5 and clinicopathological features and prognosis of gastric cancer. The relationship between NLRC5 and Wnt / 尾 -catenin signaling pathway was studied. Methods the expression of NLRC5 and 尾 -catenin was detected by immunohistochemistry in 93 paraffin specimens of patients with radical gastrectomy, and the relationship between the expression of NLRC5 and clinicopathological features of gastric cancer was analyzed. The transfection efficiency of pEGFP-C2-NLRC5 was verified by pEGFP-C2-NLRC5 recombinant plasmid pEGFP-C2-NLRC5 transfected with MKN-45 and MGC-803 by Western blot and RT-PCR respectively. The function of NLRC5 was analyzed by MTT cell proliferation and scratch assay. The effect of NLRC5 expression on the biological behavior of gastric cancer cells was observed in vitro. The changes of Wnt- 尾 -catenin signaling pathway related proteins were detected by Western blot assay. Results the positive expression of NLRC5 in 93 cases of gastric cancer was 72.04%, and the negative expression of NLRC5 was 27.96% in 26 cases, and the TNM stage of gastric cancer. The results of Kaplan-merier survival analysis showed that the prognosis of patients with negative expression of NLCR5 was better than that of patients with positive expression of P0.05. 93 cases of gastric cancer. 63 cases of positive expression of 尾 -catenin and 30 cases of negative expression of 尾 -catenin were analyzed by multivariate regression analysis. The positive expression of NLRC5 and 尾 -catenin, Low TNM staging and lymph node metastasis are independent risk factors for prognosis of gastric cancer patients. After overexpression of NLRC5 in gastric cancer cells, MTT cell proliferation and scratch test showed that, The cell proliferation and migration were significantly enhanced by P0.05P0.01P0.01Western blot. The results showed that the expression of 尾 -catenin, C-mycand metalloproteinase-7 in Wnt/ 尾 -catenin signaling pathway was significantly increased. Conclusion NLRC5 is widely expressed in gastric carcinoma. Kaplan-merier survival analysis showed that the high expression of 1% NLRC5 was a risk factor for the prognosis of gastric cancer. Cox multivariate regression analysis showed that the high expression of 1% NLRC5 was an independent risk factor for the prognosis of gastric cancer. The expression of NLRC5 was positively correlated with the expression of 尾 -catenin. The expression of NLRC5 affected the biological behavior of gastric cancer cells and the expression of key proteins in Wnt- 尾 -catenin signaling pathway. These results suggest that NLRC5 may influence the invasion and metastasis of gastric cancer by regulating Wnt- 尾 -catenin signaling pathway.
【作者單位】： 安徽醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院病理學(xué)教研室;安徽醫(yī)科大學(xué)第一附屬醫(yī)院普外科;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):81572305) 安徽省自然科學(xué)基金(編號(hào):KJ2017A204)
【分類號(hào)】：R735.2
，

本文編號(hào)：2015238