本文選題：胃癌 + TFF2��； 參考：《川北醫(yī)學院》2017年碩士論文

【摘要】：目的:檢測胃癌組織中TFF2蛋白的表達及患者幽門螺桿菌感染情況,研究TFF2蛋白表達、幽門螺桿菌感染與胃癌患者臨床病理特征之間的關(guān)系,探討TFF2蛋白表達及幽門螺桿菌感染在胃癌患者中的臨床意義。方法:收集四川省人民醫(yī)院2010年12月-2012年1月入院確診并首次行胃癌手術(shù)患者82例作為研究對象。應(yīng)用免疫組化方法檢測胃癌組織及正常胃組織中TFF2蛋白表達情況,應(yīng)用免疫組化方法檢測胃癌患者幽門螺旋桿菌的感染情況,分析TFF2蛋白表達、幽門螺桿菌感染與胃癌患者臨床病理之間的關(guān)系。并對患者進行臨床病理資料及隨訪資料分析,采用Kaplan-Meier方法計算患者生存率,Log-Rank檢驗進行單因素分析,Cox比例風險回歸進行多因素分析TFF2表達、幽門螺桿菌感染與胃癌患者預(yù)后的關(guān)系。結(jié)果:1.胃癌患者臨床病理特征病例總數(shù)82例。其中男55例,女27例,男女比例為2.0:1。年齡37-82歲,平均年齡59.2±11.5歲。臨床TNM分期:I期14例,II期19例,III期42例,IV期7例。腫瘤浸潤深度方面,以T3最多,其中T1期10例,T2期8例,T3期60例,T4期4例。無淋巴結(jié)轉(zhuǎn)移32例,有淋巴結(jié)轉(zhuǎn)移50例。胃癌病理分化程度:中低分化55例,高分化27例。腫瘤大小:≤4cm 47例,4cm 35例。2.TFF2蛋白表達、HP感染與臨床病理之間的關(guān)系TFF2蛋白主要表達在胞質(zhì)上。在30例正常胃組織標本中TFF2陰性率和陽性率百分比為16.7%(5/30)、83.3%(25/30)。在82例患者胃癌組織標本中TFF2表達陰性率和陽性率百分比分別為45.1%(37/82)、54.9%(45/82)。二者比較,胃癌組織中TFF2表達比正常胃組織降低,差異有統(tǒng)計學意義(P值0.05)。將胃癌患者各臨床特征與TFF2表達的相關(guān)性進行分析,發(fā)現(xiàn)TFF2的表達與組織分化程度有關(guān),差異有統(tǒng)計學意義(P值0.05)。在82例胃癌患者中,HP感染陽性病例50例,HP陰性病例32例,HP感染陽性率為61.0%。將胃癌患者各臨床特征與HP感染相關(guān)性進行分析,發(fā)現(xiàn)HP的感染與患者TNM分期和浸潤深度有關(guān),差異有統(tǒng)計學意義(P值0.05)。3.預(yù)后分析單因素分析顯示年齡、TNM分期、淋巴結(jié)轉(zhuǎn)移、浸潤深度、分化程度、腫瘤大小及TFF2蛋白表達、HP感染是影響胃癌患者生存的相關(guān)因素。進一步Cox比例風險模型多因素分析顯示淋巴結(jié)轉(zhuǎn)移、腫瘤大小、TFF2表達、幽門螺桿菌感染是影響胃癌患者預(yù)后的獨立因素。結(jié)論:1.TFF2在胃癌組織中的表達比正常胃組織中明顯降低。TFF2的表達與腫瘤分化程度有關(guān)。幽門螺桿菌感染與腫瘤浸潤深度、TNM分期有關(guān)。2.TFF2的表達、幽門螺桿菌感染可以作為胃癌患者預(yù)后的獨立因素,檢測胃癌組織TFF2蛋白的表達、患者幽門螺桿菌感染情況可能對判斷患者預(yù)后有幫助。3.TFF2在胃癌組織中的表達與患者幽門螺桿菌感染之間呈負相關(guān),提示二者之間可能存在某種相互作用機制影響胃癌的發(fā)生發(fā)展。
[Abstract]:Objective: to detect the expression of TFF2 protein in gastric cancer and the infection of Helicobacter pylori, and to study the relationship between the expression of TFF2 protein, the relationship between Helicobacter pylori infection and the clinicopathological features of gastric cancer. To investigate the expression of TFF2 protein and the clinical significance of Helicobacter pylori infection in patients with gastric cancer. Methods: from December 2010 to January 2012, 82 patients with gastric cancer were enrolled in Sichuan Provincial people's Hospital. Immunohistochemical method was used to detect the expression of TFF2 protein in gastric cancer tissues and normal gastric tissues. The infection of Helicobacter pylori in gastric cancer patients was detected by immunohistochemical method, and the expression of TFF2 protein was analyzed. Relationship between Helicobacter pylori infection and clinicopathology in patients with gastric cancer. The clinicopathological data and follow-up data of the patients were analyzed. The survival rate was calculated by Kaplan-Meier method. The expression of TFF2 was analyzed by univariate analysis and Cox proportional risk regression. Relationship between Helicobacter pylori infection and prognosis in patients with gastric cancer. The result is 1: 1. There were 82 cases of gastric cancer with clinicopathological features. There were 55 males and 27 females, with a ratio of 2.0: 1. The average age was 59.2 鹵11.5 years. There were 14 cases of stage II and 19 cases of stage III in TNM stage I. There were 42 cases with stage IV and 7 cases with stage IV. In terms of depth of tumor invasion, T3 was the most frequent, of which 10 cases were in T1 stage and 8 cases in T _ 2 stage, 60 cases were in T _ 3 stage and 4 cases were in T _ 4 stage. There were 32 cases without lymph node metastasis and 50 cases with lymph node metastasis. The pathological differentiation degree of gastric cancer: 55 cases with middle and low differentiation and 27 cases with high differentiation. Tumor size: 鈮，

本文編號：1956896