本文選題：DEPDC7 + 宣威　； 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]肺癌的發(fā)病率及死亡率占所有惡性腫瘤之首。云南宣威地區(qū)肺癌發(fā)病率和死亡率均居全國首位。研究宣威地區(qū)肺癌獨特的發(fā)病機(jī)制與特異的內(nèi)在驅(qū)動基因可為其治療和預(yù)防提供新的干預(yù)靶點。前期研究中,我們采用AgilentmRNA芯片發(fā)現(xiàn)DEPDC7 (DEP domain-containing protein7)在宣威和非宣威非小細(xì)胞肺癌中存在差異表達(dá)。本課題旨在組織蛋白水平初步探討DEPDC7在宣威和非宣威地區(qū)非小細(xì)胞肺癌患者的癌和癌旁正常肺組織及各臨床病理參數(shù)之間的相關(guān)性,初步闡述DEPDC7在宣威非小細(xì)胞肺癌中可能存在的臨床應(yīng)用價值。[方法]收集2009年1月至2015年12月在昆明醫(yī)科大學(xué)第三附屬醫(yī)院(云南省腫瘤醫(yī)院)行根治性切除術(shù)的165例非小細(xì)胞肺癌患者,其中宣威地區(qū)63例,非宣威地區(qū)102例。通過制作石蠟包埋和病理組織切片,采用免疫組織化學(xué)的方法檢測DEPDC7在宣威、非宣威地區(qū)非小細(xì)胞肺癌和癌旁正常組織中的表達(dá)部位及強(qiáng)度,并統(tǒng)計其表達(dá)情況與各臨床病理參數(shù)之間的相關(guān)性。[結(jié)果]1. DEPDC7在非小細(xì)胞肺癌和正常肺組織中均有表達(dá),其主要表達(dá)于細(xì)胞質(zhì)和細(xì)胞核,少量表達(dá)于線粒體。2. DEPDC7在非小細(xì)胞肺癌組織中表達(dá)顯著高于癌旁正常組織,差異具有明顯統(tǒng)計學(xué)意義(68.5% VS 41.8% P0.05)3.分層分析發(fā)現(xiàn)DEPDC7在肺腺癌中表達(dá)高于肺鱗癌(75.2%VS50%P0.05),低年齡組表達(dá)明顯高于高年齡組(68.1% VS 56.9%P0.05),在Ⅰ期患者陽性表達(dá)率高于Ⅱ+ⅢA(87.5%VS 77.1%P0.05),差異均具有統(tǒng)計學(xué)意義。4. DEPDC7在宣威非小細(xì)胞肺癌組織中表達(dá)顯著高于非宣威非小細(xì)胞肺癌組織,差異具有統(tǒng)計學(xué)意義(85.7% VS 57.8% P0.05)5. DEPDC7在宣威女性肺腺癌組織中表達(dá)顯著高于非宣威女性腺癌組織,差異具有統(tǒng)計學(xué)意義(92%VS61.1% p0.05)[結(jié)論]1. DEPDC7在非小細(xì)胞肺癌組織中高表達(dá),提示DEPDC7可能與非小細(xì)胞肺癌發(fā)生有關(guān),尤其是在肺腺癌的發(fā)生中可能起重要作用。在臨床Ⅰ期患者中高表達(dá),提示其可能參與非小細(xì)胞肺癌早期發(fā)生,因此,DEPDC7極有可能成為非小細(xì)胞肺癌早診的分子標(biāo)記。2. DEPDC7在宣威非小細(xì)胞肺癌癌組織中顯著高表達(dá),提示DEPDC7可能是宣威地區(qū)非小細(xì)胞肺癌的新候選癌基因,尤其是在宣威女性肺腺癌的發(fā)生發(fā)展中可能起重要作用。
[Abstract]:Objective: the incidence and mortality of lung cancer is the highest among all malignant tumors. The morbidity and mortality of lung cancer in Xuanwei area of Yunnan Province ranked first in China. The study of the unique pathogenesis and specific intrinsic driving gene of lung cancer in Xuanwei area may provide a new intervention target for its treatment and prevention. In previous studies, AgilentmRNA microarray was used to detect the differential expression of DEPDC7 domain-containing protein 7 in Xuan Wei and non Xuan Wei non-small cell lung cancer (NSCLC). The aim of this study was to explore the correlation of DEPDC7 in cancer and adjacent normal lung tissues of patients with non-small cell lung cancer (NSCLC) in Xuanwei and non-Xuanwei areas and the clinicopathological parameters. The clinical application value of DEPDC7 in Xuanwei non-small cell lung cancer was discussed. [methods] from January 2009 to December 2015, 165 patients with non-small cell lung cancer (NSCLC) underwent radical resection in the third affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), including 63 cases in Xuanwei area and 102 cases in non-Xuanwei area. The expression and intensity of DEPDC7 in non-small cell lung cancer (NSCLC) and adjacent normal tissues in Xuanwei and non-Xuanwei were detected by immunohistochemical method by making paraffin-embedded and pathological tissue sections. The correlation between the expression and the clinicopathological parameters was analyzed. [result] 1. DEPDC7 was expressed in both non-small cell lung cancer (NSCLC) and normal lung tissues, mainly in cytoplasm and nucleus, and a little in mitochondria. The expression of DEPDC7 in non-small cell lung cancer tissues was significantly higher than that in adjacent normal tissues, and the difference was statistically significant (68.5% vs 41.8%, P 0.05%, P 0.05). Stratified analysis showed that the expression of DEPDC7 in lung adenocarcinoma was higher than that in lung squamous cell carcinoma (VS50), the expression of DEPDC7 in low age group was significantly higher than that in high age group (68.1% vs 56.9%), and the positive rate in stage 鈪，

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