本文選題：胃癌 + 長(zhǎng)鏈非編碼RNA　； 參考：《南京醫(yī)科大學(xué)》2016年博士論文

【摘要】：胃癌作為全球第四大癌癥,嚴(yán)重威脅到人類健康,特別是對(duì)于發(fā)展中國(guó)家。世界每年胃癌新發(fā)病例近百萬(wàn),發(fā)展中國(guó)家占近2/3,其中42%發(fā)生在中國(guó)。胃癌的早期診斷與治療對(duì)患者的病情及預(yù)后至關(guān)重要,也是國(guó)內(nèi)胃癌領(lǐng)域有待突破的主要研究方向。胃癌的發(fā)生發(fā)展涉及多基因異常調(diào)控、多步驟參與,最終使細(xì)胞生物學(xué)行為由正常演變?yōu)楫惓�。研究發(fā)現(xiàn)胃癌發(fā)生發(fā)展與基因有著密切的聯(lián)系胃癌體內(nèi)的某些特定基因與正常人的基因相關(guān)比發(fā)生了變化,有可能這些變異的基因是導(dǎo)致胃癌的關(guān)鍵。當(dāng)細(xì)胞行為異常是基于基因水平發(fā)生變化時(shí),便可引發(fā)細(xì)胞結(jié)構(gòu)和功能的不可逆性改變,很大可能造成細(xì)胞癌變。胃癌的遠(yuǎn)處轉(zhuǎn)移及復(fù)發(fā)也是胃癌患者死亡的最主要原因,是影響臨床療效和預(yù)后的關(guān)鍵因素。深入研究胃癌的發(fā)病機(jī)制以及引起胃癌發(fā)生轉(zhuǎn)移的具體分子機(jī)制,可以為探索新的診斷方法及靶向治療位點(diǎn)提供堅(jiān)實(shí)的理論基礎(chǔ),也為胃癌的早期診斷和預(yù)后評(píng)價(jià)提供依據(jù)。既往針對(duì)于胃癌發(fā)生發(fā)展的機(jī)制研究多集中于傳統(tǒng)的蛋白編碼基因,主要涉及遺傳學(xué),表觀遺傳學(xué)以及相關(guān)信號(hào)通路的調(diào)控。隨著高通量測(cè)序技術(shù)發(fā)展,研究者已經(jīng)揭示在人類基因組中非編碼RNA(Non-coding RNA, ncRNA)比例占據(jù)了95%以上,蛋白編碼RNA以及功能性非編碼RNA間復(fù)雜相互作用在胃癌的發(fā)生發(fā)展中具有重要的作用。長(zhǎng)鏈非編碼LNA(long non-coding RNA, IncRNA)作為ncRNA的重要組成成分之一,也越來(lái)越受到重視。目前已經(jīng)揭示lncRNA可以通過(guò)多種途徑參與表觀遺傳,基因轉(zhuǎn)錄及轉(zhuǎn)錄后水平的調(diào)控,并且具有作為早期疾病診斷標(biāo)記物的潛力。近年來(lái),非編碼RNA尤其是長(zhǎng)鏈非編碼RNA(Long non-coding RNA, lncRNA)在腫瘤的發(fā)生機(jī)制研究中越來(lái)越受到研究者的關(guān)注。目前為止,已有眾多IncRNA被報(bào)道與腫瘤細(xì)胞增殖,侵襲轉(zhuǎn)移及腫瘤復(fù)發(fā)高度相關(guān)。同樣,在胃癌研究中,已有報(bào)道發(fā)現(xiàn)在胃癌組織與癌旁組織中具有明顯差異表達(dá)的IncRNA譜,并且某些IncRNA,如H19,HULC可通過(guò)不同機(jī)制調(diào)控下游靶基因影響胃癌細(xì)胞的增殖或侵襲能力進(jìn)而參與胃癌的發(fā)生發(fā)展。但目前關(guān)于IncRNA在胃癌中的研究尚停留在起步階段,探索和發(fā)現(xiàn)新的IncRNA及其具體調(diào)控機(jī)制,可以為胃癌的發(fā)病機(jī)制研究提供新的理論依據(jù)。長(zhǎng)鏈非編碼RNA是指一類轉(zhuǎn)錄本長(zhǎng)度大于200個(gè)核苷酸的RNA,位于細(xì)胞核內(nèi)或胞漿內(nèi),不參與或很少參與編碼蛋白,主要以RNA的形式在表觀遺傳、轉(zhuǎn)錄及轉(zhuǎn)錄后等層面上調(diào)控基因表達(dá)水平。LncRNA主要參與了基因組印記,染色體沉默以及染色質(zhì)修飾、ml RNA轉(zhuǎn)錄激活、轉(zhuǎn)錄干擾,及轉(zhuǎn)錄后調(diào)控、核內(nèi)運(yùn)輸、調(diào)節(jié)原癌基因活化等多種重要的cis-或trans-調(diào)控過(guò)程,它不但可活化某些蛋白編碼基因表達(dá),亦可以抑制蛋白編碼基因的表達(dá)。目前研究進(jìn)展已經(jīng)揭示lncRNA具有作為疾病早期診斷標(biāo)記物的潛力。本研究中我們利用生物信息學(xué)分析、表達(dá)譜芯片結(jié)合經(jīng)典分子生物學(xué)技術(shù)等,在分子水平、細(xì)胞水平、實(shí)驗(yàn)動(dòng)物水平以及臨床樣本中探索異常表達(dá)的長(zhǎng)鏈非編碼RNALinc00152對(duì)胃癌生物學(xué)行為的影響及機(jī)制。通過(guò)Affymetrix lncRNA microarray基因芯片檢測(cè)三例胃癌患者癌與癌旁組織中l(wèi)ncRNA的表達(dá)水平,芯片結(jié)果顯示：與癌旁組織相比,有271條lncRNA呈現(xiàn)不同程度的差異表達(dá),提示lncRNA可能參與胃癌的發(fā)生發(fā)展。進(jìn)一步確定芯片結(jié)果的可靠性,研究組再將其他學(xué)者已經(jīng)報(bào)道的胃癌lncRNA表達(dá)譜結(jié)果采用針對(duì)差異lncRNA聯(lián)合比對(duì)分析發(fā)現(xiàn),有3條lncRNA在兩組芯片中均有顯著差異表達(dá)。其中,長(zhǎng)鏈非編碼Linc00152在兩組芯片結(jié)果中差異水平最大,且各組內(nèi)表達(dá)一致性較好,結(jié)果較穩(wěn)定。研究組再以72例胃癌患者的癌組織和癌旁組織為實(shí)驗(yàn)樣本,采用qRT-PCR進(jìn)行Linc00152表達(dá)驗(yàn)證。結(jié)果發(fā)現(xiàn),Linc00152在癌組織中呈現(xiàn)與芯片結(jié)果一致的高表達(dá)狀態(tài)。結(jié)合患者的臨床信息發(fā)現(xiàn),Linc00152的表達(dá)水平與胃癌患者的腫瘤大小呈明顯正相關(guān)關(guān)系,而與TNM分期及淋巴結(jié)轉(zhuǎn)移等相關(guān)性不明顯。提示linc00O152可能參與胃癌細(xì)胞增殖有關(guān),據(jù)此,研究組刪選出linc00152高表達(dá)的胃癌細(xì)胞株(HGC-27, MGC803)建立體外細(xì)胞模型進(jìn)行后續(xù)功能機(jī)制研究。為明確Linc00152的亞細(xì)胞定位,研究組采用胞漿-胞核分離RNA提取方法,分別提取兩種細(xì)胞細(xì)胞的細(xì)胞漿及細(xì)胞核內(nèi)RNA,通過(guò)特異性反轉(zhuǎn)錄方法檢測(cè)兩種細(xì)胞亞單位內(nèi)LincOO152表達(dá)水平,結(jié)果顯示,Linc00152主要存在與細(xì)胞漿中,僅有少許在細(xì)胞核中表達(dá)。為明確該段序列符合長(zhǎng)鏈非編碼RNA的非編碼特性,研究組采用生物信息學(xué)軟件預(yù)測(cè)方法,確定其非編碼的生物學(xué)特性。采用目前為止公認(rèn)的長(zhǎng)鏈非編碼RNAMEG3作為陽(yáng)性對(duì)照,結(jié)果顯示,Linc00152呈現(xiàn)明顯非編碼特性。為探索Linc00152對(duì)細(xì)胞功能的影響,我們用shRNA研究Linc00152的敲除效果,用CCK8檢測(cè)、EDU實(shí)驗(yàn)研究對(duì)細(xì)胞增殖的影響。再用體外實(shí)驗(yàn)驗(yàn)證Linc00152表達(dá)與腫瘤生長(zhǎng)的相關(guān)性。用RNA拉下實(shí)驗(yàn)預(yù)測(cè)探索潛在的結(jié)合蛋白,并與反義Linc00152比較,發(fā)現(xiàn)位于約140 kDa附近的蛋白質(zhì)豐富。進(jìn)一步質(zhì)譜鑒定表明,表皮生長(zhǎng)因子受體是由Linc00152所捕獲的蛋白質(zhì)。為進(jìn)一步探索Linc00152對(duì)EGFR具體調(diào)控機(jī)制,我們首先利用RNA免疫共沉淀技術(shù)(RNA Immunoprecipitation,RIP)探索Linc00152是否可以通過(guò)直接綁定EGFR蛋白進(jìn)而影響其功能及相關(guān)效應(yīng)產(chǎn)生。RIP研究顯示Linc00152直接與EGF R蛋白結(jié)合。最終研究表明Linc00152可能通過(guò)EGFR依耐性信號(hào)通路促進(jìn)胃癌細(xì)胞生長(zhǎng)以及侵襲-轉(zhuǎn)移級(jí)聯(lián)反應(yīng),證明Linc00152在胃癌的發(fā)生發(fā)展中發(fā)揮著重要作用。綜上所述,我們的研究對(duì)胃癌的發(fā)生發(fā)展,特別是腫瘤生長(zhǎng)及轉(zhuǎn)移中,功能性非編碼RNA參與調(diào)控的機(jī)制進(jìn)行了深入而廣泛的探討,為進(jìn)一步理解該過(guò)程中紛繁復(fù)雜的信號(hào)通路提供了新的思路和視角,同時(shí),為將來(lái)胃癌的早期診斷及靶向治療提供堅(jiān)實(shí)的理論基礎(chǔ)。
[Abstract]:As the fourth largest cancer in the world, gastric cancer is a serious threat to human health, especially in developing countries. There are nearly a million new cases of gastric cancer in the world every year, and the developing countries account for nearly 2/3. 42% of them occur in China. Early diagnosis and treatment of gastric cancer are important to the patients' condition and prognosis, and are also the main breakthroughs in the field of domestic gastric cancer. The occurrence and development of gastric cancer involves the regulation of polygenic abnormalities, multi step participation, and the eventual evolution of cell biological behavior from normal to abnormal. The key to gastric cancer is the cause of gastric cancer. When the abnormal cell behavior is based on the change of gene level, it can cause irreversible changes in cell structure and function, which may lead to cell carcinogenesis. The distant metastasis and recurrence of gastric cancer is the most important cause of death of gastric cancer patients. It is the key factor affecting the clinical efficacy and prognosis. The study of the pathogenesis of gastric cancer and the specific molecular mechanisms that cause the metastasis of gastric cancer can provide a solid theoretical basis for exploring new diagnostic methods and target therapy sites, and also provide the basis for early diagnosis and prognosis evaluation of gastric cancer. Genes, mainly involved in genetics, epigenetics and regulation of related signaling pathways. With the development of high throughput sequencing technology, researchers have revealed that the proportion of non coded RNA (Non-coding RNA, ncRNA) in the human genome occupies more than 95%, the complex interaction between protein encoded RNA and functional non coded RNA is occurring in the occurrence of gastric cancer. The long chain non coding LNA (long non-coding RNA, IncRNA), as one of the important components of ncRNA, is also becoming more and more important. It has been revealed that lncRNA can be involved in epigenetic, gene transcription and post transcriptional regulation in a variety of ways, and it has the potential as a marker for early diagnosis of disease. In recent years, non coded RNA, especially long chain non coded RNA (Long non-coding RNA, lncRNA), has attracted more and more attention in the study of the pathogenesis of tumor. So far, many IncRNA have been reported to be related to tumor cell proliferation, invasion and metastasis and tumor recurrence. Also, there have been reports in the research of gastric cancer. There is a distinct differential expression of IncRNA spectrum in gastric cancer tissues and adjacent tissues, and some IncRNA, such as H19, HULC can regulate the proliferation or invasion of gastric cancer cells by different mechanisms by different mechanisms, and then participate in the development of gastric cancer. However, the research on IncRNA in gastric cancer is still in the initial stage. The new IncRNA and its specific regulatory mechanism can provide a new theoretical basis for the study of the pathogenesis of gastric cancer. Long chain non coding RNA refers to a class of RNA, which is more than 200 nucleotides in length, is located in the nucleus or in the cytoplasm, and does not participate in or rarely participate in the encoding protein, mainly in the form of epigenetic, transcriptional and post transcriptional in the form of RNA. The level of gene expression level.LncRNA is mainly involved in genomic imprinting, chromosome silence and chromatin modification, ML RNA transcription activation, transcriptional interference, post transcriptional regulation, nuclear transport, and regulating the activation of proto oncogene activation in many important cis- or trans- processes. It can not only activate the expression of some protein encoding genes, but also can be used for gene expression. The current research progress has revealed the potential of lncRNA as a marker for early diagnosis of disease. In this study, we use bioinformatics analysis, expression spectrum chips and classical molecular biology techniques to explore abnormal molecular level, cell level, experimental animal level and clinical samples. The effect of long chain non coding RNALinc00152 on biological behavior of gastric cancer and its mechanism. The expression level of lncRNA in cancer and para cancerous tissues of three patients with gastric cancer was detected by Affymetrix lncRNA microarray gene chip. The results showed that 271 lncRNA showed different degrees of differential expression compared with para cancerous tissue, suggesting lncRNA Can participate in the development of gastric cancer. Further determine the reliability of the results of the chip. The research group then found that 3 lncRNA were significantly different in the two groups of microarrays. The results of the lncRNA expression profile of gastric cancer have been reported by other scholars. Among them, the long chain non coded Linc00152 is in two groups of chip results. In the study group, the cancer tissue and the paracancerous tissue in 72 cases of gastric cancer were used as the experimental samples, and the Linc00152 expression was verified by qRT-PCR. The results showed that Linc00152 showed high expression in the cancer tissue in accordance with the results of the chip. It was found that the expression level of Linc00152 was positively correlated with the tumor size of gastric cancer patients, but the correlation with TNM staging and lymph node metastasis was not obvious. It suggested that linc00O152 might be involved in the proliferation of gastric cancer cells. Accordingly, the study group selected the linc00152 high expression of gastric cancer cell line (HGC-27, MGC803) to establish the cell model in vitro. In order to identify the subcellular location of Linc00152, the study group took the cytoplasm and nucleus separation RNA extraction method to extract the cytoplasm and the RNA in the nucleus of two cell cells, and detected the level of LincOO152 in the subunits of the two cells by the specific reverse transcription method. The results showed that the main presence of Linc00152 was fine and fine. In the cytoplasm, only a few are expressed in the nucleus. In order to clarify the non coding characteristics of this segment consistent with the long chain non coded RNA, the research group uses the bioinformatics software prediction method to determine its non coding biological characteristics. The long chain non coded RNAMEG3 is used as a positive control, and the results show that Linc00152 is obviously not present. In order to explore the effect of Linc00152 on cell function, we used shRNA to study the knockout effect of Linc00152, the effect of CCK8 test on the proliferation of cells, and the correlation between the expression of Linc00152 and the growth of the tumor in vitro. The binding protein of the detects was predicted by the RNA pull down experiment, and the ratio of the antisense Linc00152 to the antisense Linc00152 ratio was predicted. More protein was found near about 140 kDa. Further mass spectrometry identification showed that the epidermal growth factor receptor was a protein captured by Linc00152. In order to further explore the specific regulation mechanism of Linc00152 on EGFR, we first explored whether Linc00152 can be passed by RNA immunoprecipitation (RIP) technique. Direct binding of EGFR protein then affects its function and related effects resulting in.RIP studies showing that Linc00152 is directly associated with EGF R protein. The final study shows that Linc00152 may promote the growth of gastric cancer cells and the invasion and metastasis cascade reaction through the EGFR dependent signaling pathway, which proves that Linc00152 plays an important role in the development of gastric cancer. To sum up, our research has made a thorough and extensive study of the mechanism of the involvement of functional non coded RNA in the development of gastric cancer, especially in tumor growth and metastasis. It provides new ideas and perspectives for further understanding of the complicated and complex signaling pathways in the process, at the same time, for the early diagnosis and targeting of gastric cancer. Treatment provides a solid theoretical basis.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：R735.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 魯慧英;Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients[J];Chinese Medical Journal;2000年12期

2 ;Detection the HCV RNA by PCR-microplate hybridization method from clinical specimens[J];中國(guó)輸血雜志;2001年S1期

3 付漢江,鄭曉飛;類mRNA RNA研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(分子生物學(xué)分冊(cè));2002年04期

4 Verheyden B ,徐其武;口服脊髓灰質(zhì)炎疫苗核殼和RNA的穩(wěn)定性[J];國(guó)外醫(yī)學(xué).預(yù)防.診斷.治療用生物制品分冊(cè);2002年01期

5 CMBE譯文組;探索小RNA的功能[J];現(xiàn)代臨床醫(yī)學(xué)生物工程學(xué)雜志;2004年03期

6 沈維干;RNA interference and its current application in mammals[J];Chinese Medical Journal;2004年07期

7 ;Potent and specific inhibition of SARS-CoV antigen expression by RNA interference[J];Chinese Medical Journal;2005年09期

8 孫娣;汪洋;張麗娟;閆玉清;;一種簡(jiǎn)捷提取植物總RNA的方法[J];黑龍江醫(yī)藥;2005年06期

9 熊慧華;于世英;胡廣原;莊亮;;Effects of Survivin Expression Suppressed by Short Hairpin RNA on MCF-7 Cells[J];華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)英德文版);2006年03期

10 楊益超;;RNA干擾及其在醫(yī)學(xué)中的應(yīng)用[J];廣西醫(yī)學(xué);2007年10期

相關(guān)會(huì)議論文 前10條

1 金由辛;;面向21世紀(jì)的RNA研究[A];面向21世紀(jì)的科技進(jìn)步與社會(huì)經(jīng)濟(jì)發(fā)展（下冊(cè)）[C];1999年

2 ;第四屆RNA全國(guó)研討會(huì)大會(huì)報(bào)告日程安排[A];第四屆全國(guó)RNA進(jìn)展研討會(huì)論文集[C];2005年

3 ;Function of Transfer RNA Modifications in Plant Development[A];植物分子生物學(xué)與現(xiàn)代農(nóng)業(yè)——全國(guó)植物生物學(xué)研討會(huì)論文摘要集[C];2010年

4 王峰;張秋平;陳金湘;;棉花總RNA的快速提取方法[A];中國(guó)棉花學(xué)會(huì)2011年年會(huì)論文匯編[C];2011年

5 關(guān)力;陳本iY;iJ云虹;郭培芝;魏重琴;邱蘇吾;苗健;;關(guān)于動(dòng)物}D~T中RNAn,定方法的研究[A];中國(guó)生理科學(xué)會(huì)學(xué)術(shù)會(huì)議論文摘要匯編（生物化學(xué)）[C];1964年

6 夏海濱;;小RNA在免疫學(xué)領(lǐng)域中的應(yīng)用研究進(jìn)展[A];中國(guó)免疫學(xué)會(huì)第五屆全國(guó)代表大會(huì)暨學(xué)術(shù)會(huì)議論文摘要[C];2006年

7 ;The stability of hepatitis C virus RNA in various handling and storage conditions[A];中國(guó)輸血協(xié)會(huì)第四屆輸血大會(huì)論文集[C];2006年

8 郭德銀;;RNA干擾在病毒研究和控制中的應(yīng)用[A];2006中國(guó)微生物學(xué)會(huì)第九次全國(guó)會(huì)員代表大會(huì)暨學(xué)術(shù)年會(huì)論文摘要集[C];2006年

9 甘儀梅;楊業(yè)華;王學(xué)奎;曹燕;楊特武;;棉花總RNA快速提取[A];中國(guó)棉花學(xué)會(huì)2007年年會(huì)論文匯編[C];2007年

10 ;Identification and characterization of novel interactive partner proteins for PCBP1 that is a RNA-binding protein[A];中國(guó)優(yōu)生優(yōu)育協(xié)會(huì)第四屆全國(guó)學(xué)術(shù)論文報(bào)告會(huì)暨基因科學(xué)高峰論壇論文專輯[C];2008年

相關(guān)重要報(bào)紙文章 前10條

1 記者 馮衛(wèi)東;研究人員發(fā)現(xiàn)可破壞腫瘤抑制基因的小RNA[N];科技日?qǐng)?bào);2009年

2 記者 儲(chǔ)笑抒 通訊員 盛偉;人體微小RNA有望提前發(fā)出癌癥預(yù)警[N];南京日?qǐng)?bào);2011年

3 瀘州醫(yī)學(xué)院副教授、科普作家 周志遠(yuǎn);“大頭兒子”與環(huán)狀RNA[N];第一財(cái)經(jīng)日?qǐng)?bào);2014年

4 麥迪信;小分子RNA可能有大作用[N];醫(yī)藥經(jīng)濟(jì)報(bào);2003年

5 董映璧;美發(fā)現(xiàn)基因調(diào)控可回應(yīng)“RNA世界”[N];科技日?qǐng)?bào);2006年

6 張忠霞;特制RNA輕推一下,就能“喚醒”基因[N];新華每日電訊;2007年

7 聶翠蓉;RNA：縱是配角也精彩[N];科技日?qǐng)?bào);2009年

8 馮衛(wèi)東;RNA干擾機(jī)制首次在人體中獲得證實(shí)[N];科技日?qǐng)?bào);2010年

9 馮衛(wèi)東　王小龍;英在地球早期環(huán)境模擬條件下合成類RNA[N];科技日?qǐng)?bào);2009年

10 記者 常麗君;新技術(shù)讓研究進(jìn)入單細(xì)胞內(nèi)RNA的世界[N];科技日?qǐng)?bào);2011年

相關(guān)博士學(xué)位論文 前10條

1 王趙瑋;昆蟲(chóng)RNA病毒復(fù)制及昆蟲(chóng)抗病毒天然免疫機(jī)制研究[D];武漢大學(xué);2014年

2 包純;一類新非編碼RNA的發(fā)現(xiàn)以及產(chǎn)生和功能的初探[D];華中師范大學(xué);2015年

3 李語(yǔ)麗;基于MeRIP-seq的水稻RNA m6A甲基化修飾的研究[D];中國(guó)科學(xué)院北京基因組研究所;2015年

4 熊瑜琳;miR-122靶位基因STAT3調(diào)控長(zhǎng)鏈非編碼 RNA Lethe促進(jìn)HCV復(fù)制的機(jī)制研究[D];第三軍醫(yī)大學(xué);2015年

5 范春節(jié);高通量測(cè)序鑒定毛竹小RNA及其功能分析[D];中國(guó)林業(yè)科學(xué)研究院;2012年

6 王加強(qiáng);小鼠著床前胚胎特異ERV相關(guān)長(zhǎng)非編碼RNA的定向篩選及功能研究[D];東北農(nóng)業(yè)大學(xué);2015年

7 王業(yè)偉;非編碼RNA SPIU的結(jié)構(gòu)和功能研究和p19INK4D在APL發(fā)病中的作用[D];上海交通大學(xué);2013年

8 鄒艷芬;子癇前期中非編碼RNA對(duì)滋養(yǎng)細(xì)胞功能的調(diào)控及機(jī)制探索[D];南京醫(yī)科大學(xué);2015年

9 朱喬;miR-10b在人肝細(xì)胞肝癌發(fā)生中的作用及其機(jī)制的初步探索[D];第四軍醫(yī)大學(xué);2015年

10 蔣俊鋒;長(zhǎng)鏈非編碼RNA BACE1-AS促進(jìn)Aβ聚集及其調(diào)節(jié)BACE1和SERF1a的ceRNA機(jī)制研究[D];第二軍醫(yī)大學(xué);2015年

相關(guān)碩士學(xué)位論文 前10條

1 全弘揚(yáng);長(zhǎng)鏈非編碼RNA在細(xì)胞內(nèi)質(zhì)網(wǎng)應(yīng)激反應(yīng)中的相關(guān)作用及機(jī)制研究[D];北京協(xié)和醫(yī)學(xué)院;2015年

2 胡亮;DDX19A識(shí)別PRRSV基因組RNA并激活NLRP3炎癥小體[D];中國(guó)農(nóng)業(yè)科學(xué)院;2015年

3 雷文婕;小菜蛾不同發(fā)育時(shí)期RNA編輯位點(diǎn)的識(shí)別與驗(yàn)證[D];南京農(nóng)業(yè)大學(xué);2014年

4 周燕;RNA干擾對(duì)大鯢蛙病毒（CGSRV）主要功能基因表達(dá)與增殖影響的研究[D];四川農(nóng)業(yè)大學(xué);2015年

5 石新新;改進(jìn)的RNA-Seq數(shù)據(jù)轉(zhuǎn)錄組表達(dá)分析研究[D];南京航空航天大學(xué);2015年

6 陳金梅;利用植物表達(dá)藥用干擾小RNA的研究[D];南京大學(xué);2014年

7 郭維超;miR-17家族在腫瘤生長(zhǎng)和遷移中的作用及機(jī)制[D];杭州師范大學(xué);2016年

8 沈曉彤;RNA“一步法”檢測(cè)的酶學(xué)基礎(chǔ)及凝血酶等溫?cái)U(kuò)增檢測(cè)方法的研究[D];青島科技大學(xué);2016年

9 孫文陽(yáng);豬miR-15b前體單堿基突變對(duì)其生物加工過(guò)程的影響[D];甘肅農(nóng)業(yè)大學(xué);2016年

10 郅淑引;微小RNA25在肺癌血清中的表達(dá)量與臨床意義的研究[D];山西醫(yī)科大學(xué);2016年

，

本文編號(hào)：1933315