本文選題：同步熒光光譜 + 色氨酸殘基�。� 參考：《光譜學(xué)與光譜分析》2017年09期

【摘要】：用同步熒光光譜法評(píng)估原發(fā)性肝細(xì)胞癌患者血漿、肝癌荷瘤小鼠以及培養(yǎng)細(xì)胞(HepG2和HL-7702)中酪氨酸(Tyr)和色氨酸(Trp)殘基水平變化。固定發(fā)射波長(zhǎng)λem和激發(fā)波長(zhǎng)λex之間的波長(zhǎng)差Δλ分別為20和60nm,激發(fā)和發(fā)射單色器同時(shí)進(jìn)行掃描,確定350nm為Trp的同步特征發(fā)射峰位置,318nm為Tyr的同步特征發(fā)射峰位置。結(jié)果表明,肝癌患者及荷瘤小鼠血漿蛋白質(zhì)所含Tyr和Trp殘基的熒光強(qiáng)度明顯增加。相反,肝癌細(xì)胞或荷瘤小鼠腫瘤組織中Tyr和Trp殘基熒光強(qiáng)度卻隨生長(zhǎng)時(shí)間增長(zhǎng)而減少。進(jìn)一步實(shí)驗(yàn)表明,具有抗癌活性的苦參堿處理癌細(xì)胞后,細(xì)胞Tyr和Trp殘基的熒光強(qiáng)度升高。這些結(jié)果表明,Tyr和Trp殘基的變化可能參與了腫瘤的發(fā)生發(fā)展。
[Abstract]:The changes of tyrosine (TRP) and tryptophan (Trp) residues in plasma of hepatocellular carcinoma (HCC) mice and cultured cells (HepG2 and HL-7702) were evaluated by synchronous fluorescence spectrometry. The wavelength difference 螖 位 between constant emission wavelength 位 em and excitation wavelength 位 ex is 20 and 60 nm, respectively. The excitation and emission Monochromator scan at the same time and determine that 350nm is the position of synchronous characteristic emission peak of Trp and position of synchronous characteristic emission peak of Tyr. The results showed that the fluorescence intensity of Tyr and Trp residues in plasma proteins of HCC patients and tumor-bearing mice were significantly increased. In contrast, the fluorescence intensity of Tyr and Trp residues in tumor tissues of hepatoma cells or tumor-bearing mice decreased with the increase of growth time. Further experiments showed that the fluorescence intensity of Tyr and Trp residues increased after matrine treatment with anticancer activity. These results suggest that the changes of Tyr and Trp residues may be involved in tumorigenesis and progression.
【作者單位】： 汕頭大學(xué)醫(yī)學(xué)院生物分析實(shí)驗(yàn)室;汕頭大學(xué)醫(yī)學(xué)院第二附屬醫(yī)院急診科;
【基金】：廣東省科學(xué)技術(shù)廳-廣東省中醫(yī)藥科學(xué)院聯(lián)合科研專項(xiàng)(2012A032500009)資助
【分類號(hào)】：O657.3;R730.43
，

本文編號(hào)：1925115