本文選題：維生素C碳點(diǎn) + 口腔黏膜��； 參考：《吉林大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年04期

【摘要】：目的:研究維生素C碳點(diǎn)對口腔黏膜鱗狀細(xì)胞癌(口腔鱗癌)KB細(xì)胞的殺傷作用,探討其相關(guān)作用機(jī)制。方法:以不同濃度(5、10、20、40和80mg·L~(-1))維生素C碳點(diǎn)體外處理口腔鱗癌KB細(xì)胞作為實(shí)驗(yàn)組,以0mg·L~(-1)維生素C碳點(diǎn)組作為空白對照組。MTT法檢測各組細(xì)胞增殖率,克隆形成實(shí)驗(yàn)檢測各組細(xì)胞克隆形成能力,Western blotting法檢測各組細(xì)胞中自噬相關(guān)蛋白LC3蛋白表達(dá)水平,流式細(xì)胞術(shù)檢測KB細(xì)胞的凋亡率。結(jié)果:與空白對照組比較,20、40和80mg·L~(-1)維生素C碳點(diǎn)組KB細(xì)胞的增殖率及克隆形成能力均明顯降低(P0.01),40mg·L~(-1)維生素C碳點(diǎn)組KB細(xì)胞中LC3Ⅱ蛋白表達(dá)水平和細(xì)胞凋亡率明顯升高(P0.05或P0.01)。結(jié)論:維生素C碳點(diǎn)能夠有效地殺傷口腔鱗癌KB細(xì)胞,抑制KB細(xì)胞的增殖并減弱其克隆形成能力,其殺傷作用可能與KB細(xì)胞自噬和凋亡的發(fā)生有關(guān)。
[Abstract]:Aim: to study the killing effect of vitamin C carbon spot on KB cells in oral squamous cell carcinoma (OSCC) and its related mechanism. Methods: KB cells of oral squamous cell carcinoma were treated with Vitamin C carbon spots at different concentrations of 5, 10, 20, 40 and 80mg, respectively. The proliferation rate of KB cells was measured by 0mg LX-1) Vitamin C carbon spot group as a blank control group. The proliferation rate of KB cells in each group was measured by MTT assay. The expression of autophagy associated protein (LC3) protein was detected by Western blotting assay and the apoptosis rate of KB cells was detected by flow cytometry. Results: compared with the control group, the proliferation rate and clone formation ability of KB cells in the vitamin C carbon spot group were significantly lower than those in the control group. The expression level of LC3 鈪，

本文編號：1890469