本文選題：骨肉瘤 + 全反式維甲酸�。� 參考：《第三軍醫(yī)大學(xué)學(xué)報》2017年13期

【摘要】：目的研究全反式維甲酸(all-trans-retinoic acid,ATRA)與骨形態(tài)發(fā)生蛋白9(bone morphogenetic proteins 9,BMP9)對人骨肉瘤細(xì)胞增殖及分化的影響。方法利用四甲基偶氮唑藍(lán)(MTT)法和流式細(xì)胞儀分析細(xì)胞增殖情況;蛋白質(zhì)免疫印跡實(shí)驗(yàn)(Western blot)檢測細(xì)胞增殖和成骨相關(guān)指標(biāo);半定量PCR及Western blot分析ATRA和ATRA與BMP9聯(lián)用處理143B細(xì)胞株后對BMP9表達(dá)水平的影響。結(jié)果在人骨肉瘤143B細(xì)胞株中,與對照組相比,ATRA能明顯抑制細(xì)胞增殖(P0.05),并促使細(xì)胞周期阻滯在G1期(P0.05),下調(diào)PCNA的蛋白水平,并呈濃度依賴性;ATRA能呈濃度依賴性增加晚期成骨指標(biāo)OCN和OPN的蛋白水平(P0.05);ATRA能上調(diào)BMP9的m RNA及蛋白水平,并呈濃度依賴性(P0.05);單用BMP9并不能上調(diào)OCN和OPN表達(dá),并促進(jìn)其增殖(P0.05),但與ATRA聯(lián)用時,能夠增強(qiáng)ATRA誘導(dǎo)的相關(guān)成骨指標(biāo)表達(dá)和抗增殖作用(P0.05)。結(jié)論ATRA對143B細(xì)胞的增殖具有抑制作用并誘導(dǎo)其成骨分化,并可能恢復(fù)BMP9的成骨誘導(dǎo)能力。
[Abstract]:Objective to study the effects of all-trans-retinoic acid ATRAA and bone morphogenetic protein 9(bone morphogenetic proteins 9 on proliferation and differentiation of human osteosarcoma cells. Methods the cell proliferation was analyzed by MTT and flow cytometry, and the cell proliferation and osteoblast related indexes were detected by Western blot assay. The effect of ATRA, ATRA and BMP9 on the expression of BMP9 in 143B cell line was analyzed by semi-quantitative PCR and Western blot. Results in human osteosarcoma cell line 143B, compared with the control group, PCNA could significantly inhibit the proliferation of human osteosarcoma cell line P0.05A, promote cell cycle arrest at G1 phase, and down-regulate the protein level of PCNA. In a concentration-dependent manner, ATRA could increase the protein levels of OCN and OPN in a concentration-dependent manner. ATRA could upregulate m RNA and protein levels of BMP9 in a concentration-dependent manner, but BMP9 alone could not up-regulate the expression of OCN and OPN. In combination with ATRA, the expression of osteogenic markers induced by ATRA and the anti-proliferative effect of P0.05 could be enhanced. Conclusion ATRA can inhibit the proliferation of 143B cells and induce the osteogenic differentiation of 143B cells, and may restore the osteogenic ability of BMP9.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第二醫(yī)院骨科;重慶市生物化學(xué)與分子藥理學(xué)重點(diǎn)實(shí)驗(yàn)室;重慶醫(yī)科大學(xué)藥學(xué)院藥理學(xué)教研室;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81572226)~~
【分類號】：R738

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