本文選題：青蒿琥酯 + 人結(jié)腸癌細(xì)胞HCT116和HT29��； 參考：《山西醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:觀察青蒿琥酯對(duì)人結(jié)腸癌細(xì)胞HCT116和HT29抑制和凋亡的影響,為日后臨床結(jié)直腸癌(CRC)的治療提供可行的新的輔助化療治療手段。方法:用不同濃度青蒿琥酯處理兩種人結(jié)腸癌細(xì)胞HCT116和HT29,應(yīng)用四甲基偶氮唑藍(lán)(MTT)比色法在酶標(biāo)儀490nm波長處測(cè)量每孔的吸光度(A)值,檢測(cè)青蒿琥酯對(duì)人結(jié)腸癌細(xì)胞HCT116和HT29的抑制效應(yīng);應(yīng)用流式細(xì)胞術(shù)(FCM)檢測(cè)人結(jié)腸癌細(xì)胞HCT116和HT29細(xì)胞凋亡的情況。使用SPSS13.0統(tǒng)計(jì)軟件處理數(shù)據(jù),實(shí)驗(yàn)數(shù)據(jù)(即細(xì)胞抑制率和凋亡率)采用于均數(shù)±標(biāo)準(zhǔn)差(`X±s)表示,HCT116和HT29抑制率與凋亡率組內(nèi)兩兩比較應(yīng)用兩樣本t檢驗(yàn),組內(nèi)抑制率和凋亡率的比較分別用單因素方差分析和最小差異t測(cè)試(LSD-t)分析、濃度與抑制率兩者關(guān)系采用雙變量線性相關(guān)分析。P0.05,差異具有統(tǒng)計(jì)學(xué)意義。結(jié)果:不同濃度青蒿琥酯(7.5、15、30、60、120μg·ml-1)處理兩種人結(jié)腸癌細(xì)胞48 h后,抑制率隨濃度增加呈上升趨勢(shì)(HCT116:19.42%、24.59%、44.84%、74.15%、92.87%;HT29:11.77%、17.63%、34.23%、48.51%、70.59%);且同種濃度下,對(duì)HCT116作用明顯強(qiáng)于HT29。不同濃度青蒿琥酯(0、60、120μg·ml-1)處理兩種人結(jié)腸癌細(xì)胞24 h后凋亡率分別為(HCT116:2.46%51.36%64.10%;HT29:1.14%35.95%43.45%),加藥組與對(duì)照組、同濃度下兩種細(xì)胞凋亡均有統(tǒng)計(jì)學(xué)意義。結(jié)論:青蒿琥酯對(duì)人結(jié)腸癌細(xì)胞HCT116和HT29具有生長抑制作用,與濃度曾正相關(guān)作用,且對(duì)HCT116作用強(qiáng)于HT29;青蒿琥酯對(duì)人結(jié)腸癌細(xì)胞HCT116和HT29具有誘導(dǎo)凋亡作用,且對(duì)HCT116作用強(qiáng)于HT29。
[Abstract]:Aim: to observe the effect of artesunate on the inhibition and apoptosis of HCT116 and HT29 in human colon cancer cells, and to provide a new adjuvant chemotherapy for the treatment of colorectal cancer. Methods: two kinds of human colon cancer cell lines HCT116 and HT29 were treated with artesunate of different concentrations. The absorbance of each hole was measured by MTT colorimetry at the wavelength of 490nm. The inhibitory effect of artesunate on HCT116 and HT29 in human colon cancer cells and apoptosis in HCT116 and HT29 cells were detected by flow cytometry. SPSS13.0 statistical software was used to process the data. The experimental data (that is, cell inhibition rate and apoptosis rate) were measured by mean 鹵standard deviation (`X 鹵s). The inhibition rates of HCT116 and HT29 were compared with those in the apoptotic rate group. Two samples t test were used to compare the inhibition rate of HCT116 and HT29. Intra-group inhibition rate and apoptosis rate were analyzed by single factor ANOVA and least difference t test (LSD-t). The relationship between concentration and inhibition rate was analyzed by bivariate linear correlation analysis. P0.05. the difference was statistically significant. Results: after treated with different concentrations of artesunate 7.51530C 60120 渭 g ml-1 for 48 h, the inhibition rate of HCT116: 19.42C24.5959and HCT116: 19.42ML-1 was higher than that of HT292911.7717.637.2378.51.The inhibitory rate of HCT116: 19.42C24.5945 was better than that of HT299.The inhibition rate of HCT116: 19.42 + 24.59mg / ml was much stronger than that of HT299.The inhibitory effect of HCT116: 19.42C24.590.59 was better than that of HT29. Two kinds of human colon cancer cells were treated with different concentrations of artesunate (60120 渭 g ml-1) for 24 hours. The apoptotic rates of two kinds of human colon cancer cells were as follows: HCT116: 2.46Q.36d.10and HT29: 1.145.95C.450.There were significant differences between the two groups. Conclusion: artesunate can inhibit the growth of human colon cancer cell line HCT116 and HT29, which is positively related to the concentration of artesunate, and has stronger effect on HCT116 than HT29.The artesunate can induce apoptosis in human colon cancer cell line HCT116 and HT29, and the effect on HCT116 is stronger than that on HCT116.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.35

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