本文選題：肺腫瘤 + miR-　； 參考：《實(shí)用醫(yī)學(xué)雜志》2017年01期

【摘要】：目的:探討miR-126、miR-355及Exportin-5在肺癌中表達(dá)及相互之間關(guān)系。方法:收集47例肺癌患者的癌組織及癌旁組織,利用TRIzol提取總RNA,使用定時(shí)熒光定量PCR檢測(cè)基因的相對(duì)表達(dá)量。結(jié)果:肺癌組織與癌旁組織中的miR-126,Exportin-5差異具有統(tǒng)計(jì)學(xué)意義(P0.05),癌旁組織中miR-126與pri-miR-126(r=-0.31)呈負(fù)相關(guān)(P0.05),癌組織中基因表達(dá)量與臨床指標(biāo)之間的關(guān)系中發(fā)現(xiàn)miR-126與MRP(r=0.43)呈正相關(guān)(P0.05),Exportin-5與TOPO(r=-0.36)、pri-miR-126與飲酒(r=-0.34)、MRP(r=-0.43)呈負(fù)相關(guān)(P0.05)。結(jié)論:miR-126、Exportin-5在肺癌組織中表達(dá)下調(diào),基因在肺癌發(fā)生發(fā)展過(guò)程中可能通過(guò)抑制基因的表達(dá)促進(jìn)肺癌的發(fā)生與發(fā)展。
[Abstract]:Objective: to investigate the expression and relationship of miR-126 miR-355 and Exportin-5 in lung cancer. Methods: the cancer tissues and adjacent tissues of 47 patients with lung cancer were collected. The total RNAs were extracted by TRIzol and the relative expression of genes was detected by timed fluorescence quantitative PCR. Results: the difference of miR-126 exportin-5 between lung cancer and paracancerous tissues was statistically significant (P 0.05), and the expression of miR-126 was negatively correlated with pri-miR-126 r-0.31 (P 0.05). A positive correlation was found between miR-126 and MRPr0.43). There was a negative correlation with the alcohol consumption (r = 0.34) and MRP (r = 0.43) (P = 0.05, P = 0.05). Conclusion the expression of 1: miR-126 exportin-5 is down-regulated in lung cancer tissues, and the gene may promote the occurrence and development of lung cancer by inhibiting the expression of genes during the carcinogenesis and development of lung cancer.
【作者單位】： 昆明醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;昆明醫(yī)科大學(xué)第三附屬醫(yī)院胸心外科;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):81460491) 云南省衛(wèi)生廳內(nèi)設(shè)機(jī)構(gòu)項(xiàng)目(編號(hào):2016NS108)
【分類號(hào)】：R734.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 曾龍劍;吳錫南;楊凱云;李蘋;徐儷p，

本文編號(hào)：1818202