發(fā)布時(shí)間：2018-04-28 20:05

  本文選題：B型胸腺瘤 + 胸腺癌　； 參考：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年06期

【摘要】：目的微小RNA(microRNAs)在多種腫瘤發(fā)生發(fā)展中具有重要作用,但在胸腺上皮性腫瘤中的研究較少。文中旨在研究microRNAs在胸腺上皮性腫瘤(B3型胸腺瘤與胸腺癌)中的表達(dá)譜及其意義。方法收集南京軍區(qū)南京總醫(yī)院病理科2012年1月至2015年1月間的手術(shù)切除B3型胸腺瘤3例(對(duì)照組)及胸腺鱗狀細(xì)胞癌3例(病例組),做基因芯片研究。將病例組與對(duì)照組數(shù)據(jù)相比,計(jì)算出各miRNA的差異倍數(shù),篩選出上調(diào)與下調(diào)的miRNA。通過(guò)預(yù)測(cè)網(wǎng)站預(yù)測(cè)其靶基因,結(jié)合文獻(xiàn)檢索出與胸腺相關(guān)的基因。結(jié)果 B3型胸腺瘤與胸腺鱗狀細(xì)胞癌對(duì)比發(fā)現(xiàn),32種差異表達(dá)miRNAs在胸腺癌中上調(diào),包括miR-125b-1-3p、miR-3175、miR-4462等;19種差異表達(dá)miRNAs在胸腺癌中下調(diào),包括miR-361-5p、miR-130a-3p、miR-3651等。miR-377-5p的靶基因包括AKT1、C9、CD19、CDC42、LSS、MYC;miR-485-5p的靶基因包括ADCYAP1R1、ASPA、CAD、CD63等;miR-183-5p的靶基因包括AKAP12、CD28、FOXP1、MDM4等。結(jié)論篩選出B3型胸腺瘤與胸腺癌中的差異表達(dá)miRNAs,通過(guò)預(yù)測(cè)軟件預(yù)測(cè)這些miRNAs的靶基因,可以為胸腺上皮性腫瘤的進(jìn)一步治療提供了有效的依據(jù)。
[Abstract]:Objective RNA (microRNAs) plays an important role in the development of a variety of tumors, but there are few studies in the thymic epithelial tumors. The purpose of this study was to study the expression and significance of microRNAs in the thymic epithelial tumor (B3 type thymoma and thymic carcinoma). Methods the pathology department of the general Hospital of Nanjing, Nanjing military region, from January 2012 to 2015 1 3 cases of B3 thymoma (control group) and 3 cases of thymic squamous cell carcinoma (case group) were performed in month. Compared with the control group, the difference multiple of each miRNA was calculated, and the up regulation and down regulation of miRNA. were screened to predict the target gene through the forecast website and the genes related to the thymus were retrieved in the literature. Results compared with thymic squamous cell carcinoma, 32 different expressions of miRNAs were up-regulated in thymic carcinoma, including miR-125b-1-3p, miR-3175, miR-4462 and so on. 19 differentially expressed miRNAs were down-regulated in thymic carcinoma, including AKT1, C9, miR-130a-3p, and miR-3651, and.MiR-377-5p, including miR-361-5p, miR-130a-3p and miR-3651. The target genes include ADCYAP1R1, ASPA, CAD, CD63 and so on. The target genes of miR-183-5p include AKAP12, CD28, FOXP1, MDM4 and so on. Conclusion the differential expression of B3 type thymoma and thymic carcinoma is miRNAs. The prediction software can predict the target genes of these miRNAs, which can provide an effective basis for the further treatment of thymic epithelial tumors.

【作者單位】： 南方醫(yī)科大學(xué)金陵醫(yī)院(南京軍區(qū)南京總醫(yī)院)病理科;安徽省立醫(yī)院南區(qū)病理科;
【基金】：國(guó)家自然科學(xué)基金(81372743)
【分類(lèi)號(hào)】：R736.3

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