本文選題：超聲波穿孔效應(yīng) + 金屬納米顆粒　； 參考：《福建師范大學(xué)》2015年碩士論文

【摘要】：本論文闡述了超聲波穿孔效應(yīng)的細(xì)胞SERS光譜研究。針對(duì)現(xiàn)有將金屬納米粒子導(dǎo)入活細(xì)胞中方法上存在的缺陷,我們首次開展了超聲波穿孔效應(yīng)-SERS光譜技術(shù)研究,即通過超聲波穿孔效應(yīng)將金屬納米顆粒(金和銀)快速(1分鐘)導(dǎo)入活細(xì)胞中作為SERS增強(qiáng)基底,獲得細(xì)胞的SERS光譜,從而實(shí)現(xiàn)對(duì)細(xì)胞內(nèi)各物質(zhì)生化成分的研究及多種癌細(xì)胞的檢測(cè)和篩查應(yīng)用。主要研究成果如下：1、利用超聲波穿孔效應(yīng)將銀納米顆�？焖賹�(dǎo)入K562、NP69及C666細(xì)胞,結(jié)合超聲波穿孔后的細(xì)胞TEM圖像驗(yàn)證了超聲波穿孔效應(yīng)確實(shí)能夠使得金屬納米顆�？焖龠M(jìn)入細(xì)胞內(nèi)；2、通過細(xì)胞超聲波穿孔效應(yīng)-SERS光譜的分析獲得了最佳的優(yōu)化測(cè)試條件：采用20倍物鏡并將光斑擴(kuò)束到100%；3、利用超聲波穿孔效應(yīng)法與傳統(tǒng)孵育內(nèi)吞法下獲得全細(xì)胞SERS光譜存在很大差異,可能跟銀納米顆粒具有毒性作用有關(guān)；4、通過超聲波穿孔效應(yīng)-SERS技術(shù)獲得的正常鼻咽NP69細(xì)胞與鼻咽癌C666細(xì)胞的SERS光譜,從而進(jìn)行癌癥細(xì)胞的篩查研究；其次,用同樣方法對(duì)比分析NP69與K562細(xì)胞；最后再進(jìn)行三種細(xì)胞的區(qū)分篩查與分析。實(shí)驗(yàn)結(jié)果表明,超聲波穿孔效應(yīng)-SERS技術(shù)方法可以在1分鐘內(nèi)將金和銀納米顆粒快速導(dǎo)入活細(xì)胞內(nèi)部并作為SERS增強(qiáng)基底,通過檢測(cè)細(xì)胞SERS光譜,可快速確定細(xì)胞的生化成分,并應(yīng)用于癌細(xì)胞的篩查研究。該方法不僅具有操作簡(jiǎn)便快速、分辨率高等優(yōu)點(diǎn),而且擴(kuò)展了SERS技術(shù)在細(xì)胞生化研究領(lǐng)域的應(yīng)用,其應(yīng)用前景十分廣闊。
[Abstract]:In this paper, the cell SERS spectroscopy of ultrasonic perforation is described. In view of the defects of the existing methods for the introduction of metal nanoparticles into living cells, we have carried out the first research on ultrasonic perforation effect (SERS) spectroscopy. In other words, the metal nanoparticles (gold and silver) were introduced into living cells as SERS enhancement substrate by ultrasonic piercing effect for 1 minute, and the SERS spectra of the cells were obtained. Thus, the biochemical components of various substances in the cell and the detection and screening of many kinds of cancer cells can be realized. The main results are as follows: 1. Silver nanoparticles were rapidly introduced into K562NP-69 and C666 cells by ultrasonic perforation. Combined with the TEM images of cells after ultrasonic perforation, it was proved that the ultrasonic perforation effect could make metal nanoparticles enter the cell rapidly. The best optimized measurement was obtained by the analysis of cell ultrasonic perforation effect -SERS spectra. The test conditions were as follows: using 20 times objective lens and expanding the light spot to 100 ~ 3, the SERS spectra of whole cell obtained by ultrasonic perforation method and traditional incubation endocytosis method were very different. It may be related to the toxic effect of silver nanoparticles. The SERS spectra of normal nasopharyngeal NP69 cells and nasopharyngeal carcinoma C666 cells obtained by ultrasonic perforation effect -SERS technique were used to screen cancer cells. NP69 and K562 cells were compared with each other by the same method. The experimental results show that the ultrasonic perforation effect -SERS technique can quickly transfer gold and silver nanoparticles into living cells within one minute and act as a SERS enhancement substrate. The biochemical components of the cells can be quickly determined by detecting the SERS spectra of the cells. And applied to the screening of cancer cells. This method not only has the advantages of simple and fast operation and high resolution, but also extends the application of SERS technology in the field of cell biochemistry, and its application prospect is very broad.
【學(xué)位授予單位】：福建師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R730.4;O657.3

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本文編號(hào)：1810833