本文選題：EFEMP + 子宮內(nèi)膜癌��； 參考：《山東大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年10期

【摘要】：目的探討包含表皮生長因子的fibulin類細胞外基質(zhì)2(EFEMP2)在子宮內(nèi)膜癌組織以及子宮內(nèi)膜癌細胞中的表達及其臨床意義。方法用免疫組織化學(xué)法檢測200例子宮內(nèi)膜癌組織以及40例正常子宮內(nèi)膜組織中EFEM P2的表達;采用免疫細胞化學(xué)法、qRT-PCR、Western blotting檢測EFEM P2在4種子宮內(nèi)膜癌細胞(KLE、HEC-1A、HEC-1B、Ishikaw a)中的表達水平;運用生長曲線及軟瓊脂克隆形成實驗檢測子宮內(nèi)膜癌細胞的增殖能力;運用Transwell體外侵襲及轉(zhuǎn)移實驗檢測子宮內(nèi)膜癌細胞的侵襲及轉(zhuǎn)移能力。結(jié)果 EFEMP2在子宮內(nèi)膜癌組織、細胞中的表達明顯低于正常子宮內(nèi)膜組織及細胞(P0.001),且與子宮內(nèi)膜癌晚期、分化程度低、淋巴結(jié)轉(zhuǎn)移密切相關(guān)(P0.05)。KLE和HEC-1A的增殖、侵襲、轉(zhuǎn)移能力高于HEC-1B和Ishikawa(P0.001)。結(jié)論EFEM P2的表達與子宮內(nèi)膜癌臨床分期、組織分化程度、有無淋巴結(jié)轉(zhuǎn)移和侵襲生長等惡性表型密切相關(guān),并可能參與抑制子宮內(nèi)膜癌侵襲、轉(zhuǎn)移過程。
[Abstract]:Objective to investigate the expression and clinical significance of fibulin extracellular matrix 2 (EFEMP2) containing epidermal growth factor (ECM) in endometrial carcinoma and endometrial cancer cells. Methods immunohistochemical staining was used to detect the expression of EFEM P2 in 200 endometrial carcinoma tissues and 40 normal endometrium tissue; immunocytochemistry was used. The expression level of EFEM P2 in 4 endometrial carcinoma cells (KLE, HEC-1A, HEC-1B, Ishikaw a) was detected by method, qRT-PCR and Western blotting. The proliferation ability of endometrial carcinoma cells was detected by the growth curve and the soft agar clone formation test. The invasion and metastasis of endometrial carcinoma cells were detected by the Transwell invasion and metastasis test. Results EFEMP2 was significantly lower than normal endometrium and cells (P0.001) in endometrial carcinoma tissue, and with advanced endometrium, low differentiation, lymph node metastasis closely related (P0.05).KLE and HEC-1A proliferation, invasion, metastasis ability higher than HEC-1B and Ishikawa (P0.001). Conclusion the expression of EFEM P2 and the uterus of Ishikawa (P0.001). The clinical stage of endometrial carcinoma and the degree of tissue differentiation are closely related to the malignant phenotype of lymph node metastasis and invasive growth, and may be involved in inhibiting the invasion and metastasis of endometrial carcinoma.

【作者單位】： 山東大學(xué)公共衛(wèi)生學(xué)院兒少衛(wèi)生與婦幼保健系;
【分類號】：R737.33

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