本文選題：鱘魚硫酸軟骨素 + 結(jié)直腸癌��； 參考：《食品科學(xué)》2017年21期

【摘要】：探究鱘魚硫酸軟骨素對結(jié)直腸癌細(xì)胞的生長抑制能力,并初步確定影響結(jié)直腸癌細(xì)胞生長的主要原因。選取3株具有代表性的結(jié)直腸癌細(xì)胞Caco-2、HCT-116、SW480,采用CCK-8法測定細(xì)胞生長曲線、硫酸軟骨素對癌細(xì)胞增殖的抑制能力;以其中一株結(jié)直腸癌細(xì)胞HCT-116為模型,用流式細(xì)胞儀測定其周期和凋亡情況,用細(xì)胞核染色法觀察處理前后細(xì)胞形態(tài),結(jié)合caspase-3凋亡酶活力的測定,考察鱘魚硫酸軟骨素對結(jié)直腸癌細(xì)胞的促凋亡能力,并初步確定作用途徑。研究結(jié)果表明,鱘魚硫酸軟骨素對結(jié)直腸癌細(xì)胞Caco-2、HCT-116、SW480的增殖具有一定抑制作用,最高抑制率分別為70.94%、90.00%和75.00%,明顯高于陽性對照處理組;處理后,細(xì)胞周期G1/G0期比例升高至88.56%,S期比例降低至4.47%,阻滯細(xì)胞G1/G0期;同時,使用鱘魚硫酸軟骨素處理細(xì)胞后,細(xì)胞形態(tài)發(fā)生變化,出現(xiàn)細(xì)胞核固縮以及細(xì)胞破碎等現(xiàn)象,細(xì)胞主要的凋亡酶活力顯著升高,酶活力可達(dá)1 645 IU/μg,使細(xì)胞發(fā)生凋亡,凋亡率可達(dá)63.73%。研究結(jié)果證明,鱘魚硫酸軟骨素具有促進(jìn)結(jié)直腸癌細(xì)胞凋亡、抑制細(xì)胞增殖的能力。
[Abstract]:To investigate the inhibitory effect of chondroitin sulfate on the growth of colorectal cancer cells, and to determine the main reasons that affect the growth of colorectal cancer cells. Three representative colorectal cancer cell lines, Caco-2HCT-116C480, were selected to determine the cell growth curve by CCK-8 assay and the inhibitory effect of chondroitin sulfate on the proliferation of cancer cells, one of which was used as a model for colorectal cancer cell line HCT-116. Cell cycle and apoptosis were measured by flow cytometry, cell morphology was observed by nuclear staining before and after treatment, and the apoptotic ability of Sturgeon chondroitin sulfate on colorectal cancer cells was studied by combining the activity of caspase-3 apoptotic enzyme. The action path was preliminarily determined. The results showed that chondroitin sulfate of Sturgeon had a certain inhibitory effect on the proliferation of colorectal cancer cell line Caco-2HCT-116 and SW480, and the highest inhibitory rates were 70.944% and 75.00%, respectively, which were significantly higher than those in the positive control group. The proportion of G1/G0 phase in cell cycle increased to 88.56 and the proportion of S phase decreased to 4.47%, which blocked the cell G1/G0 phase. At the same time, after treated with chondroitin sulfate from Sturgeon, the cell morphology changed, cell nucleus pyknosis and cell fragmentation occurred. The activity of the main apoptotic enzyme increased significantly, and the activity of the main apoptotic enzyme reached 1 645 IU/ 渭 g. The apoptotic rate was 63.73%. The results showed that chondroitin sulfate from Sturgeon had the ability of promoting apoptosis and inhibiting cell proliferation.
【作者單位】： 內(nèi)蒙古大學(xué)生命科學(xué)學(xué)院;中國農(nóng)業(yè)大學(xué)食品科學(xué)與營養(yǎng)工程學(xué)院;
【基金】：北京市鱘魚、鮭鱒魚創(chuàng)新團(tuán)隊(duì)項(xiàng)目(SCGWZJ201711)
【分類號】：R73-36

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