本文選題：孕激素 + PR-M��； 參考：《現(xiàn)代婦產(chǎn)科進展》2017年01期

【摘要】：目的:研究孕激素對孕激素受體M(PR-M)陽性原代子宮肌瘤細(xì)胞生長的作用,并探討其機制。方法:收集2015年9月到2016年6月在鄭州大學(xué)第三附屬醫(yī)院婦科因子宮肌瘤行全子宮切除的肌瘤標(biāo)本,原代培養(yǎng)細(xì)胞成功后用Western blot分別檢測篩選出PR-M(+)/PR-A/B(-)的標(biāo)本作為實驗組和PR-M(-)/PR-A/B(+)作為對照組。用不同濃度米非司酮(MIF)處理,培養(yǎng)48h后,應(yīng)用MTT法和流式細(xì)胞儀分別檢測其對PR-M陽性和PR-M陰性子宮肌瘤細(xì)胞增殖和凋亡的影響。結(jié)果:隨著濃度的增加與作用時間的延長,PR-M(+)和PR-M(-)子宮肌瘤細(xì)胞生長均受到抑制,濃度≥1×10~(-6)mol/L MIF處理48h后,兩組肌瘤細(xì)胞的增殖抑制率和凋亡率逐漸增加,呈濃度和劑量依賴性(均0.05)。其中PR-M(+)組肌瘤細(xì)胞各濃度增殖抑制率和凋亡率均小于PR-M(-)組,差異有統(tǒng)計學(xué)意義(均0.05)。結(jié)論:MIF對PR-M原代培養(yǎng)子宮肌瘤細(xì)胞凋亡的作用和抑制增殖的作用時間-劑量依賴性。孕激素可能通過PR-M抑制原代培養(yǎng)子宮肌瘤細(xì)胞的凋亡促進肌瘤的發(fā)生發(fā)展,這種作用可被孕激素受體拮抗劑阻斷。
[Abstract]:Aim: to study the effect of progesterone on the growth of primary uterine leiomyoma cells positive for progesterone receptor MnPR-Mand and its mechanism. Methods: from September 2015 to June 2016, the specimens of total hysterectomy in gynecology department of the third affiliated Hospital of Zhengzhou University were collected. After the primary cultured cells were successfully cultured, Western blot was used to detect and screen PR-M- (P / PR-A- / B-) samples as the experimental group and PR-M- / P / PR-A- / B- () as the control group. After 48 hours of culture, the effects of mifepristone on the proliferation and apoptosis of PR-M positive and PR-M negative uterine leiomyoma cells were detected by MTT assay and flow cytometry, respectively. Results: the growth of PR-M- () and PR-M-) uterine leiomyoma cells were inhibited with the increase of the concentration and the time of treatment. After 48 hours of treatment with 1 脳 10~(-6)mol/L MIF, the proliferation inhibition rate and apoptosis rate of the two groups increased gradually. In a dose-dependent and concentration-dependent manner (both 0.05). The proliferation inhibition rate and apoptosis rate of PR-M- group were lower than that of PR-M- group, and the difference was statistically significant (all 0.05%). Conclusion the effect of WMIF on apoptosis and proliferation of primary cultured uterine leiomyoma cells in PR-M is time and dose-dependent. Progesterone may inhibit the apoptosis of primary cultured uterine leiomyoma cells by PR-M, which can be blocked by progesterone receptor antagonist.
【作者單位】： 鄭州大學(xué)第三附屬醫(yī)院婦產(chǎn)科;河南省醫(yī)藥科學(xué)研究院;
【基金】：河南省高等學(xué)校重點科研項目計劃(No:16A320047)
【分類號】：R737.33
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本文編號：1787447