本文選題：VX + 經(jīng)肝動脈化學治療栓塞��； 參考：《中南大學學報(醫(yī)學版)》2017年11期

【摘要】：目的:評價超順磁性氧化鐵(superparamagnetic iron oxide,SPIO)納米藥物載體聯(lián)合碘化油(lipiodol,LIP)經(jīng)肝動脈栓塞治療兔VX2肝癌的可行性及效果。方法:新西蘭大白兔24只,肝內(nèi)種植VX2瘤組織制作VX2肝癌模型,將荷瘤兔隨機分為阿霉素(DOX)組、DOX-LIP組、SPIO-DOX組、SPIO-DOX-LIP組共4組,每組6只,行肝動脈栓塞治療。于術前以及術后1,3,5,7 d檢測血清ALT和AST水平;術后0,5,15,30,60,120 min檢測血清DOX濃度;術后7 d行MRI及CT掃描觀察SPIO及LIP在肝組織及其腫瘤中的分布和沉積情況;術后7 d取肝及其腫瘤組織檢測組織DOX濃度,同時行病理學檢查,包括HE染色、普魯士蘭染色及TUNEL染色,計算腫瘤壞死率和凋亡指數(shù)。結果:與DOX組相比,其他3組術后1和3 d血清AST和ALT水平均明顯升高(P0.05);術后7 d DOX組、DOX-LIP組和SPIODOX-LIP組血清AST和ALT水平恢復至術前水平,3組間差異無統(tǒng)計學意義(P0.05);SPIO-DOX-LIP組術后120 min內(nèi)各時間點的血清DOX濃度均明顯低于其他3組(P0.05);SPIO-DOX-LIP組術后7 d腫瘤組織DOX濃度明顯高于其他3組(P0.05)。術后7 d T2加權像(T2WI)示SPIO-DOX組和SPIO-DOX-LIP組腫瘤信號降低,CT示DOX-LIP組和SPIO-DOX-LIP組腫瘤區(qū)域LIP沉積。術后7 d病理結果示SPIO-DOX-LIP組腫瘤中心大部分區(qū)域凝固性壞死,腫瘤壞死率和凋亡指數(shù)均高于其他3組(P0.05)。結論:SPIO納米藥物載體聯(lián)合LIP是一種安全、可行的化學治療栓塞體系,具有良好的MRI和CT可視性,可提高兔VX2肝癌的化學藥物栓塞治療的效果。
[Abstract]:Aim: to evaluate the feasibility and effect of superparamagnetic iron oxidetio (SPIO) nano-drug carrier combined with lipiodolium lipiodolium lipiodol lipiodol (LIP) transcatheter arterial embolization (TACE) in the treatment of rabbit VX2 liver cancer. Methods: 24 New Zealand white rabbits were implanted with VX2 tumor tissue to make VX2 liver cancer model. The rabbits were randomly divided into 4 groups: SPIO-DOX-LIP group (SPIO-DOX-LIP group) and SPIO-DOX-LIP group (6 rabbits in each group). The levels of serum ALT and AST were detected before operation and 7 days after operation, the serum DOX concentration was detected at 0 ~ 5 ~ 1530T ~ 60120 min, and the distribution and deposition of SPIO and LIP in liver tissue and tumor were observed by MRI and CT scanning 7 days after operation. DOX concentration in liver and tumor tissue was measured 7 days after operation. Pathological examination including HE staining Prussian blue staining and TUNEL staining were performed to calculate tumor necrosis rate and apoptosis index. Results: compared with DOX group, The serum AST and ALT levels in the other three groups were significantly increased on the 1st and 3rd day after operation, and the serum AST and ALT levels in the DOX group and the SPIODOX-LIP group returned to the preoperative level on the 7th day after operation. There was no significant difference among the three groups in the level of serum AST and ALT in the DOX group and the SPIODOX-LIP group at each time point within 120 min after the operation in the SPIO-DOX-LIP group. The serum DOX concentration in SPIO-DOX-LIP group was significantly lower than that in the other three groups (P 0.05). The DOX concentration of tumor tissue in SPIO-DOX-LIP group was significantly higher than that in other three groups on the 7th day after operation. On the 7th day after operation, T2 weighted imaging (T2WI) showed decreased tumor signal intensity in SPIO-DOX group and SPIO-DOX-LIP group. Ct showed LIP deposition in tumor area of DOX-LIP group and SPIO-DOX-LIP group. 7 days after operation, the pathological results showed that the coagulation necrosis in most of the tumor center in SPIO-DOX-LIP group was higher than that in the other three groups (P 0.05), and the necrotic rate and apoptosis index were higher than those in the other three groups. ConclusionTwo nano-drug carrier combined with LIP is a safe and feasible chemoembolization system with good MRI and CT visibility. It can improve the effect of chemoembolization of rabbit VX2 liver cancer.
【作者單位】： 中南大學湘雅三醫(yī)院放射科;中南大學湘雅藥學院;
【基金】：湖南省科技計劃項目(2015SK20664)~~
【分類號】：R735.7

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