本文選題：Nek8 + BICD2��； 參考：《貴州醫(yī)科大學》2017年碩士論文

【摘要】：目的:探討結直腸癌(colorectal cancer,CRC)組織中Nek8的mRNA表達情況,Nek8、BICD2、VHL的蛋白表達變化及三者與CRC臨床病理特征和預后的關系。方法:采用RT-PCR(Reverse Transcription Polymerase Chain Reaction)和RT-qPCR(Real-time RT-PCR)的方法檢測30例配對的CRC組織、相應的鄰近CRC組織的癌旁組織(距離腫瘤3.0cm處)和相應的鄰近CRC組織的正常結直腸黏膜組織(距離腫瘤組織8.0-10.0cm處)中Nek8的mRNA表達情況。應用免疫組織化學的方法(SP法)檢測30例配對的CRC組織和相應的鄰近CRC組織的正常結直腸黏膜組織中Nek8、BICD2、VHL蛋白表達情況和病理學特征,同時檢測20例結直腸黏膜慢性炎癥、20例結直腸管狀腺瘤(II級)和60例結直腸管狀腺癌組織中Nek8、BICD2、VHL蛋白表達情況及與CRC臨床病理參數(shù)的關系。結果:(1)Nek8 mRNA在CRC組織和癌旁組織中的表達高于結直腸正常黏膜上皮中的表達,其中CRC組織中的表達最高。(2)免疫組化結果顯示:30例配對標本中Nek8、BICD2蛋白均在CRC組織中高表達[56.7%(17?30例)、76.7%(23?30例)]且顯著高于結直腸正常黏膜組織中的表達[13.3%(4?30例)、13.3%(4?30例)],VHL蛋白在CRC組織中的表達[30.0%(9?30例)]顯著低于結直腸正常黏膜組織[63.3%(19?30例)](p0.05);(3)Nek8和BICD2蛋白在結直腸腺瘤組織[80.0%(16?20例)、75.0%(15?20例)]及腺癌組織[83.3%(50?60例)、73.3%(44?60例)]中的表達量明顯高于結直腸黏膜慢性炎癥組織[25.0%(5?20例)、20.0%(4?20例)],VHL蛋白分別在結直腸黏膜慢性炎癥組織、腺瘤組織和腺癌組織中的陽性表達量[75.0%(15?20例)、40.0%(8?20例)、26.7%(16?60例)]呈逐漸降低的趨勢(p0.05),三種蛋白的表達分別與年齡、性別、浸潤深度、淋巴結轉移、臨床分期、五年生存率、遠處轉移無關(p0.05)。(4)對CRC組織中Nek8、BICD2、VHL分別進行兩兩比較,Nek8和BICD2兩者之間相關性顯著,呈正相關的關系(r=0.337,P0.05),余兩兩之間相關性比較均無統(tǒng)計學意義(p0.05)。結論:Nek8、BICD2在CRC組織中表達增加,VHL在CRC組織中表達減少,三者可能在CRC的發(fā)生、發(fā)展中起重要的作用。
[Abstract]:Objective: to investigate the expression of Nek8 mRNA in colorectal carcinoma and its relationship with clinicopathological features and prognosis of CRC. Methods: 30 matched CRC tissues were detected by RT-PCR(Reverse Transcription Polymerase Chain reaction and RT-qPCR(Real-time RT-PCR. The expression of Nek8 mRNA in adjacent adjacent tissues of CRC (adjacent to tumor 3.0cm) and normal colorectal mucosa adjacent to CRC (8.0-10.0cm) was observed. Immunohistochemical method was used to detect the expression and pathological features of Nek8 BICD2VHL protein in 30 matched CRC tissues and corresponding normal colorectal mucosa tissues adjacent to CRC. At the same time, the expression of Nek8 BICD2VHL protein and its relationship with clinicopathological parameters of CRC were detected in 20 cases of chronic colorectal mucosal inflammation and 20 cases of colorectal tubular adenoma grade II) and 60 cases of colorectal tubular adenocarcinoma. Results the expression of Nek8 mRNA in CRC and adjacent tissues was higher than that in normal colorectal mucosal epithelium. The expression of Nek8 BICD2 protein in 30 matched specimens of CRC was higher than that in normal colorectal mucosa tissues [56.7 ~ 1730 cases] and was significantly higher than that in normal colorectal mucosa tissue [13.3330 cases] and the expression of Nek8BICD2 protein was significantly higher than that in normal colorectal mucosa tissue [13.3330 cases [13.330 cases].] VHL protein was expressed in the tissues of CRC tissues (56.70.1730 cases), and was significantly higher than that in normal colorectal mucosa tissues (13.3330 cases (13.330 cases)). The expression of CRC in colorectal adenoma [30.030 cases] was significantly lower than that in normal colorectal mucosal tissue [63.33,1930 cases] p0.05 ~ (3) Nek8 and BICD2 protein in colorectal adenoma [80.020 cases of rectal adenoma [80.020 cases] and adenocarcinoma (83.335060 cases of "73.3")] and the expression of BICD2 protein was significantly higher than that of "chronic colorectal mucosal inflammation" (83.335060 cases). The VHL protein was found in the chronic inflammation of colorectal mucosa in 20 cases (20 cases) and 20 cases (20 cases). The positive expression level in adenoma tissue and adenocarcinoma tissue [75.0% 1520 cases (40.0%) and 820 cases (26.7% 1660 cases)] showed a decreasing trend. The expression of three proteins was correlated with age, sex, depth of invasion, lymph node metastasis, clinical stage, 5-year survival rate, respectively. There was significant correlation between Nek8 and BICD2 in CRC tissues. There was a positive correlation between Nek8 and BICD2VHL. There was a positive correlation between Nek8 and BICD2VHL, and there was no significant correlation between the other two groups. Conclusion the expression of BICD2 in CRC tissues increased and decreased in CRC tissues. The expression of BICD2 may play an important role in the occurrence and development of CRC.
【學位授予單位】：貴州醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R735.34

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