本文選題：端粒酶逆轉(zhuǎn)錄酶(hTERT) + PinX1�。� 參考：《遵義醫(yī)學(xué)院》2016年碩士論文

【摘要】：目的:檢測(cè)膽管癌患者膽汁脫落細(xì)胞中端粒酶逆轉(zhuǎn)錄酶(hTERT)、PinX1 mRNA的表達(dá),探討其對(duì)臨床應(yīng)用的價(jià)值。方法:(1)收集以梗阻性黃疸為診斷入院,并于Ⅰ期行經(jīng)皮肝穿刺膽道引流術(shù)(PTCD)治療的患者術(shù)中膽汁標(biāo)本52例。根據(jù)Ⅱ期手術(shù)治療術(shù)后病理學(xué)檢查結(jié)果,確定膽管癌患者32例(實(shí)驗(yàn)組)及膽管結(jié)石患者20例(對(duì)照組)。(2)采用反轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(reverse transcription-PCR,RT-PCR)方法檢測(cè)兩組患者膽汁脫落細(xì)胞中hTERT、PinX1 mRNA的表達(dá)。結(jié)果:(1)32例膽管癌患者中hTERT mRNA陽性23例(23/32,71.8%),20例膽管結(jié)石患者中hTERT mRNA陽性3例(3/20,15%),并且膽管癌組平均相對(duì)表達(dá)水平明顯高于膽管結(jié)石組(1.2046±0.76)vs(0.109265±0.267),兩組間有統(tǒng)計(jì)學(xué)差異(P0.05)。(2)32例膽管癌患者中PinX1 mRNA陽性11例(11/32,34.3%),20例膽管結(jié)石患者中PinX1 mRNA陽性16例(16/20,80%),并且膽管癌組平均相對(duì)表達(dá)水平明顯低于膽管結(jié)石組(0.212575±0.30141)vs(0.677995±0.35637),兩組間有統(tǒng)計(jì)學(xué)差異(P0.05)。(3)20例有淋巴結(jié)轉(zhuǎn)移的膽管癌中hTERT mRNA陽性20例(20/20,100%),12例無淋巴結(jié)轉(zhuǎn)移的膽管癌中hTERT mRNA陽性3例(3/12,25%),兩組間有統(tǒng)計(jì)學(xué)差異(P0.05);20例有淋巴結(jié)轉(zhuǎn)移的膽管癌中PinX1 mRNA陽性1例(1/20,5%),12例無淋巴結(jié)轉(zhuǎn)移的膽管癌中PinX1 mRNA陽性10例(10/12,83.3%),兩組間有統(tǒng)計(jì)學(xué)差異(P0.05)。(4)膽管癌患者膽汁脫落細(xì)胞中hTERT、PinX1 mRNA的表達(dá)呈負(fù)性相關(guān)。結(jié)論:(1)膽管癌患者膽汁脫落細(xì)胞中hTERT mRNA表達(dá)增高、PinX1 mRNA表達(dá)降低,并與膽管癌患者有無淋巴結(jié)轉(zhuǎn)移有關(guān),且兩者之間呈負(fù)相關(guān)。(2)聯(lián)合檢測(cè)膽管癌患者膽汁脫落細(xì)胞中hTERT mRNA、PinX1 mRNA的表達(dá)可作為判斷梗阻性黃疸疾病性質(zhì)、評(píng)估患者病情的一種手段。
[Abstract]:Objective: to detect the expression of telomerase reverse transcriptase (hTERT) PinX 1 mRNA in bile exfoliated cells from patients with cholangiocarcinoma and to explore its clinical value.Methods 52 intraoperative bile samples were collected from 52 patients with obstructive jaundice diagnosed and treated by percutaneous transhepatic biliary drainage (PTCD).According to the results of pathological examination after stage 鈪，

本文編號(hào)：1765018