發(fā)布時(shí)間：2018-04-17 08:19

  本文選題：舌癌 + 趨化因子　； 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2017年08期

【摘要】：目的探討β亞族趨化因子受體5(CCR5)在不同人舌鱗癌細(xì)胞株的表達(dá)情況以及其配體巨噬細(xì)胞炎癥蛋白-1β(MIP-1β)對(duì)人舌鱗癌細(xì)胞株CAL-27增殖和凋亡的影響。方法采用蛋白印跡法和免疫熒光染色對(duì)3種人舌鱗癌細(xì)胞株(CAL-27、UM-1、Tca-8113)MIP-1β的相應(yīng)受體CCR5的表達(dá)進(jìn)行檢測(cè);根據(jù)MIP-1β刺激濃度的不同,細(xì)胞被隨機(jī)分為4組:0 ng/m L組(對(duì)照組)、10 ng/m L組、20 ng/m L組、40 ng/m L組。用CCK-8試劑盒(Cell Counting Kit-8)檢測(cè)MIP-1β在刺激12、24、48 h時(shí),對(duì)CAL-27細(xì)胞增殖的影響;實(shí)驗(yàn)分組同前,分別用0、10、20、40 ng/m L IP-10處理24 h后收集細(xì)胞,用Annexin V/PI雙染流式細(xì)胞儀檢測(cè)CAL-27細(xì)胞的凋亡情況。結(jié)果 CCR5在3種人舌鱗癌細(xì)胞株均有表達(dá),并且CCR5在3株細(xì)胞的胞膜及胞質(zhì)內(nèi)均有染色;和對(duì)照組相比,MIP-1β對(duì)CAL-27細(xì)胞有促進(jìn)增殖的作用。在12 h、24 h時(shí),3種濃度的MIP-1β均能夠促進(jìn)CAL-27細(xì)胞增殖(P0.05);48 h時(shí),10 ng/m L、20 ng/m L的MIP-1β均能夠促進(jìn)CAL-27細(xì)胞增殖(P0.05),但40 ng/m L的MIP-1β對(duì)CAL-27細(xì)胞的增殖無(wú)影響(P0.05);在24 h時(shí),40 ng/m L MIP-1β組的細(xì)胞凋亡率顯著高于對(duì)照組(P0.05),而10 ng/m L、20 ng/m L MIP-1β組細(xì)胞凋亡率和對(duì)照組之間均無(wú)顯著差異(P0.05)。結(jié)論 CCR5在3種人舌鱗癌細(xì)胞株的胞膜及胞質(zhì)內(nèi)均有表達(dá);MIP-1β對(duì)CAL-27細(xì)胞有促進(jìn)增殖的作用,但相對(duì)較高濃度的MIP-1β對(duì)CAL-27細(xì)胞的凋亡也有促進(jìn)作用。隨著較高濃度MIP-1β作用時(shí)間的延長(zhǎng),促增殖作用減弱,而這種減弱可能是由MIP-1β的促凋亡作用的逐步發(fā)揮所引起的。
[Abstract]:Objective to investigate the expression of 尾 subfamily chemokine receptor 5 (CCR5) in different human tongue squamous cell carcinoma cell lines and the effect of its ligands, macrophage inflammatory protein 1 尾 (MIP-1 尾) on the proliferation and apoptosis of human tongue squamous cell carcinoma cell line CAL-27.Methods the expression of MIP-1 尾 receptor CCR5 in three human tongue squamous cell carcinoma cell lines CAL-27UM-1, Tca-8113 and MIP-1 尾 was detected by Western blotting and immunofluorescence staining.The cells were randomly divided into 4 groups: 0 ng/m / L (control group, 10 ng/m / L) and 20 ng/m / L group (40 ng/m / L).CCK-8 Counting Kit-8 was used to detect the effect of MIP-1 尾 on the proliferation of CAL-27 cells after 48 h stimulation, and the cells were collected after 24 h treatment with the same ng/m L IP-10 and Annexin V/PI double staining flow cytometry was used to detect the apoptosis of CAL-27 cells.Results CCR5 was expressed in three human tongue squamous cell carcinoma cell lines, and CCR5 was stained in the cell membrane and cytoplasm of the three cell lines, and MIP-1 尾 could promote the proliferation of CAL-27 cells compared with the control group.Three concentrations of MIP-1 尾 could promote the proliferation of CAL-27 cells at 12 h and 24 h. MIP-1 尾 of 10 ng/m L and 20 ng/m L could promote the proliferation of CAL-27 cells at 48 h. However, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells, and at 24 h, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells. At 24 h, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells, and at 24 h, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells.The rate of apoptosis was significantly higher than that of the control group (P 0.05), but there was no significant difference between the 10 ng/m L ~ + 20 ng/m L MIP-1 尾 group and the control group (P 0.05).Conclusion the expression of CCR5 in the membrane and cytoplasm of three human tongue squamous cell carcinoma cell lines can promote the proliferation of CAL-27 cells, but the relatively high concentration of MIP-1 尾 can also promote the apoptosis of CAL-27 cells.With the prolongation of high concentration of MIP-1 尾, the proliferative effect was weakened, which may be caused by the gradual exertion of the apoptotic effect of MIP-1 尾.
【作者單位】： 南方醫(yī)科大學(xué)口腔醫(yī)院//廣東省口腔醫(yī)院口腔頜面外科;南方醫(yī)科大學(xué)口腔醫(yī)院//廣東省口腔醫(yī)院牙體牙髓科;
【基金】：國(guó)家自然科學(xué)基金(81670950) 廣州市科技計(jì)劃項(xiàng)目(2014Y2-00109) 廣東省科技計(jì)劃項(xiàng)目(2014A020212549)~~
【分類號(hào)】：R739.86

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