本文選題：黑色素瘤 + 預(yù)后�。� 參考：《石河子大學(xué)》2015年碩士論文

【摘要】：目的:檢測(cè)活性誘導(dǎo)性胞嘧啶脫氨基酶(AID)在黑素瘤中的表達(dá),探討其與黑素瘤侵襲轉(zhuǎn)移、預(yù)后及BRAF突變的關(guān)系和臨床意義。方法:AID蛋白在80例黑素瘤與23例色素痣石蠟包埋組織切片中的表達(dá)采用免疫組化SP兩步法檢測(cè)。使用spss數(shù)據(jù)分析軟件分析蛋白表達(dá)情況結(jié)合臨床特性、預(yù)后等的關(guān)系。結(jié)果:黑素瘤AID蛋白的陽性表達(dá)率53.75%(43/80),色素痣的表達(dá)率13.04%(3/23),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。AID蛋白的表達(dá)與Clark分級(jí)與浸潤(rùn)深度呈正相關(guān)。伴有淋巴結(jié)轉(zhuǎn)移的黑素瘤AID蛋白的表達(dá)率高。并且,AID蛋白陽性表達(dá)的黑素瘤預(yù)后差。(P0.05),不同年齡與性別的黑素瘤患者之間AID蛋白的陽性率差異不大。不同民族患者的蛋白表達(dá)率也沒有較大差別(P0.05)。19例發(fā)生BRAF突變黑素瘤組織中AID蛋白17例陽性表達(dá),其中15例BRAFV600E突變的黑素瘤AID蛋白均陽性表達(dá)。結(jié)論:AID蛋白可能可能誘導(dǎo)了黑素瘤BRAF突變,其參與黑素瘤的侵襲、轉(zhuǎn)移并與預(yù)后密切相關(guān),提示AID蛋白是一個(gè)潛在的預(yù)后判斷指標(biāo)。BRAF突變黑素瘤中AID蛋白高表達(dá)率為黑素瘤的靶向治療提供了理論基礎(chǔ)。
[Abstract]:Objective: to investigate the expression of active inducible cytosine deaminase (idd) in melanoma and its relationship with invasion and metastasis of melanoma, prognosis and BRAF mutation.Methods the expression of AID protein in paraffin-embedded tissue sections of 80 cases of melanoma and 23 cases of nevus pigmentosa was detected by immunohistochemical SP two-step method.Spss data analysis software was used to analyze the relationship between protein expression and clinical characteristics and prognosis.Results: the positive expression rate of AID protein in melanoma was 53.75% / 80%, and the expression rate of nevus pigmented was 13.04% / 23%. There was a positive correlation between the expression of AID protein and the grade of Clark and the depth of invasion.The expression rate of AID protein in melanoma with lymph node metastasis is high.The prognosis of melanoma with positive expression of AID protein was poor. The positive rate of AID protein in melanoma patients with different age and sex was not different.There was no significant difference in the expression rate of AID protein between patients from different nationalities and 19 cases of melanoma with BRAF mutation. There were 17 cases of positive expression of AID protein in melanoma tissues with BRAFV600E mutation, among which 15 cases were positive expression of AID protein in melanoma with BRAFV600E mutation.Conclusion the BRAF mutation of melanoma may be induced by the protein of: AID, which is involved in the invasion, metastasis and prognosis of melanoma.The results suggest that AID protein is a potential prognostic marker. The high expression rate of AID protein in BRAF mutant melanoma provides a theoretical basis for targeted treatment of melanoma.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R739.5

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