本文選題：miR-370　切入點：EGFR　出處：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]1、明確miR-370、EGFR在肺癌組織中的表達水平。2、明確在肺癌組織中miR-370和EGFR之間是否有相關(guān)性。3、通過肺癌患者癌組織中miR-370的表達水平與其臨床病理特征的相關(guān)性分析,探索miR-370的表達水平是否與臨床病理特征相關(guān)及其臨床意義,為開發(fā)肺癌新的治療靶點提供理論依據(jù)。[方法]1、收集38例肺癌患者肺組織,每例患者分別取正常組織(在離腫瘤邊緣最遠端取材)和癌組織(腫瘤中心取材,避免取材壞死部分)。分別提取兩組組織中的總RNA,逆轉(zhuǎn)錄為cDNA,采用實時熒光定量PCR檢測兩組組織中miR-370和EGFR表達水平的高低,用2-△△CT計算miR-370和EGFR的相對表達量。2、分析miR-370和EGFR的表達水平是否有相關(guān)性。3、分析miR-370在肺癌患者癌組織中的表達水平與其臨床特征、腫瘤標志物及病理參數(shù)之間的關(guān)系。4、統(tǒng)計學分析:數(shù)據(jù)處理用SPSS 21.0統(tǒng)計軟件包。差異性表達用Wilcoxon符號秩檢驗進行統(tǒng)計,相關(guān)性分析用Spearman進行檢驗。[結(jié)果]1、miR-370在肺癌患者正常組織中的表達水平高于其在癌組織中的表達水平,差異有統(tǒng)計學意義(p=0.0160.05)。2、EGFR在肺癌患者癌組織中的表達水平顯著高于其在正常組織中的表達水平,差異有統(tǒng)計學意義(p=0.0080.05)。3、miR-370和EGFR在肺癌組織中的表達水平呈負相關(guān)性(r=-0.333,p=0.0030.05)。4、miR-370在肺癌患者癌組織中的表達水平越高非腺癌的傾向性越高(r=0.229,p=0.0460.05); miR-370的表達水平與NSE呈正相關(guān)(r=0.326, p=0.0240.05);與 SCC 呈正相關(guān)(r=0.285,p=0.0270.05)。5、miR-370在肺癌患者癌組織中的表達水平與患者的年齡、性別、地域、腫瘤大小、分期、淋巴結(jié)轉(zhuǎn)移和遠處轉(zhuǎn)移情況無相關(guān)性(p0.05);與CEA、CA125、CA153、CA199、CA242、CYFRA21-1等腫瘤標志物無相關(guān)性(p0.05)。[結(jié)論]1、miR-370在肺癌患者正常組織中的表達水平高于其在癌組織中的表達水平。2、EGFR在肺癌患者癌組織中的表達水平顯著高于其在正常組織中的表達水平。3、miR-370和EGFR在肺癌組織中的表達水平呈負相關(guān),提示EGFR可能是miR-370調(diào)控肺癌的一個靶基因。4、miR-370在肺癌患者癌組織中的表達水平越高非腺癌的傾向性越高,miR-370的表達水平與NSE呈正相關(guān),與SCC呈正相關(guān),提示miR-370可能與肺癌的組織學分類有關(guān)。
[Abstract]:[objective] 1. To determine the expression level of miR-370EGFR in lung cancer tissues, and to determine whether there is a correlation between miR-370 and EGFR in lung cancer tissues, and to analyze the correlation between the expression level of miR-370 and clinicopathological features of lung cancer patients.To explore whether the expression of miR-370 is related to clinicopathological features and its clinical significance, to provide theoretical basis for the development of new therapeutic targets for lung cancer.[methods] 1. The lung tissues of 38 patients with lung cancer were collected. The normal tissues (at the farthest edge of the tumor) and the cancer tissues (the tumor center) were taken from each patient to avoid necrotic parts.Total RNAs were extracted from two groups of tissues and reverse transcripted to cDNA.Real-time fluorescence quantitative PCR was used to detect the expression of miR-370 and EGFR in the two groups.The relative expression of miR-370 and EGFR was calculated by 2-CT. The correlation between miR-370 and EGFR was analyzed, and the expression of miR-370 in lung cancer was analyzed.The relationship between tumor markers and pathological parameters. 4. Statistical analysis: SPSS 21. 0 statistical software package was used for data processing.The difference expression was analyzed by Wilcoxon sign rank test and correlation analysis by Spearman.[results] 1the expression level of miR-370 in normal tissues of lung cancer was higher than that in normal tissues of lung cancer, and the difference was statistically significant (P < 0.05). The expression level of EGFR in lung cancer was significantly higher than that in normal tissues.There was a positive correlation between the expression of SCC and the age of patients with lung cancer.There was no correlation between sex, region, tumor size, stage, lymph node metastasis and distant metastasis (p0.05), but no correlation with tumor markers such as CA242CYFRA21-1.[conclusion] 1 the expression level of miR-370 in normal tissues of lung cancer patients is higher than that in normal tissues of lung cancer patients. 2EGFR expression level in cancer tissues of lung cancer patients is significantly higher than that in normal tissues. 3 miR-370 and EGFR expression level in lung cancer group.The level of expression in weaving was negatively correlated.It is suggested that EGFR may be a target gene of miR-370 regulating lung cancer. The higher the expression level of MR-370 in lung cancer is, the higher the tendency of non-adenocarcinoma is. The higher the expression level of miR-370 is, the positive correlation is between the expression of MR-370 and NSE, and the expression level of MR-370 is positively correlated with SCC.The results suggest that miR-370 may be related to the histological classification of lung cancer.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R734.2

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本文編號：1725311