本文選題：結腸癌　切入點：生物標志物　出處：《基因組學與應用生物學》2017年08期

【摘要】：為分析結腸上皮癌變進程中的差異表達蛋白質,篩選結腸癌診斷候選標志物,先采用激光捕獲顯微切割技術(LCM)純化人正常結腸組織和結腸癌組織,再采用同位素標記對蛋白質進行相對和絕對定量(i TRAQ),然后結合強陽離子柱分離和反相液質聯(lián)用(2D-LC-MS/MS)鑒定差異表達蛋白質。試驗共鑒定了1 042個非冗余蛋白,篩選出與人結腸癌變相關的差異蛋白162個,其中在結腸癌中表達上調的蛋白質68個,表達下調的蛋白質94個,功能分析表明,這些差異蛋白質涉及內質網(wǎng)蛋白質加工、糖酵解/糖原異生、黏著斑細胞連接、調節(jié)微絲組裝和細胞外基質受體相互作用等分子功能。進一步采用Western blotting及免疫組織化學技術(IHC)驗證了其中差異蛋白S100A9的表達,證實了定量蛋白質組學結果的可靠性。研究表明:差異表達蛋白質與結腸癌變相關,并有可能成為結腸癌診斷的候選分子標志物,為進一步深入研究這些蛋白質在結腸癌發(fā)生和發(fā)展進程中的分子機制,為結腸癌的發(fā)病機制研究提供新線索。
[Abstract]:In order to analyze the differentially expressed proteins in the carcinogenesis of colon epithelium and to screen the candidate markers for the diagnosis of colon cancer, the normal colon tissue and colon cancer tissue were purified by laser capture microdissection (LCM).Then the relative and absolute quantification of the protein was carried out by isotope labeling, and then the differentially expressed protein was identified by strong cationic column separation and reversed phase liquid / mass spectrometry (RLC-MS).A total of 1,042 nonredundant proteins were identified and 162 differentially expressed proteins were screened out, including 68 up-regulated proteins and 94 down-regulated proteins in colon cancer.These differential proteins are involved in the processing of endoplasmic reticulum proteins, glycolysis / glycogen heterogeneity, adhesion of plaque cells, regulation of microfilament assembly and interaction of extracellular matrix receptors, and so on.Furthermore, Western blotting and immunohistochemical technique were used to verify the expression of differential protein S100A9, which confirmed the reliability of quantitative proteomics results.Studies have shown that differentially expressed proteins are associated with colon cancer and may become candidate molecular markers for the diagnosis of colon cancer. In order to further study the molecular mechanism of these proteins in the carcinogenesis and development of colon cancer,It provides a new clue for the study of the pathogenesis of colon cancer.
【作者單位】： 中南大學湘雅醫(yī)院衛(wèi)生部腫瘤蛋白質組學重點實驗室抗癌藥物國家與地方聯(lián)合工程實驗室結構生物學與藥物設計湖南省工程實驗室;南華大學藥學與生物科學學院湖南省分子靶標新藥研究協(xié)同創(chuàng)新中心;廣西科技大學醫(yī)學院;
【基金】：國家重點基礎研究發(fā)展計劃(973計劃)(2011CB910704、2014CBA02004) 國家自然科學基金(81272971、81202129、81372516) 湖南省教育廳資助科研項目(16C1409)共同資助
【分類號】：R735.35

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