本文選題：肺腫瘤　切入點(diǎn)：SOXB轉(zhuǎn)錄因子　出處：《中國(guó)組織工程研究》2017年13期

【摘要】：背景:研究已證實(shí)干細(xì)胞轉(zhuǎn)錄因子Sox2和Oct4的高表達(dá)與肺癌的發(fā)生密切相關(guān)。目的:探討肺癌組織中Sox2和Oct4表達(dá)與腫瘤微血管生成、臨床病理指標(biāo)的相關(guān)性。方法:應(yīng)用免疫組織化學(xué)染色法檢測(cè)60例肺癌組織和60例正常組織中Sox2和Oct4的表達(dá),以及CD34標(biāo)記的微血管密度。分析Sox2,Oct4的表達(dá)和微血管密度值與臨床病理參數(shù)關(guān)系。結(jié)果與結(jié)論:(1)Sox2和Oct4在肺癌組織中陽(yáng)性表達(dá)率為46.67%(28/60)、71.67%(43/60),而在正常肺組織中表達(dá)均為陰性,差異有顯著性意義(P0.001);(2)肺癌組織中微血管密度值為16.22±2.18,明顯高于正常組織4.36±2.07,差異有顯著性意義(P0.001);(3)Sox2、Oct4和微血管密度均與腫瘤TNM分期、分化程度、脈管浸潤(rùn)、淋巴結(jié)轉(zhuǎn)移及肝轉(zhuǎn)移相關(guān)(P均0.05),而與年齡、性別無(wú)關(guān)(P均0.05);(4)肺癌組織Sox2和Oct4陽(yáng)性表達(dá)組微血管密度值顯著高于陰性組(P0.001);(5)Spearmen相關(guān)分析顯示:肺癌組織中Sox2與Oct4無(wú)明顯相關(guān)性(r=2.752,P0.05);(6)結(jié)果表明,Sox2和Oct4表達(dá)上調(diào)可作為肺癌發(fā)生的早期預(yù)警事件,并與微血管密度呈正相關(guān),二者可能共同參與了腫瘤微血管的生成、浸潤(rùn)及血行轉(zhuǎn)移。
[Abstract]:Background: the high expression of stem cell transcription factor Sox2 and Oct4 has been confirmed to be closely related to the occurrence of lung cancer.Objective: to investigate the correlation between the expression of Sox2 and Oct4 and tumor microangiogenesis and clinicopathologic indexes in lung cancer.Methods: immunohistochemical staining was used to detect the expression of Sox2 and Oct4 and the microvessel density of CD34 in 60 cases of lung cancer and 60 cases of normal tissues.The expression of Sox2 Oct4 and the relationship between microvessel density and clinicopathologic parameters were analyzed.Results and conclusion the positive expression rate of Sox2 and Oct4 in lung cancer tissues was 46.67% and 71.67% / 60% respectively, but negative in normal lung tissues.The microvessel density in lung cancer tissues was 16.22 鹵2.18, which was significantly higher than that in normal tissues (4.36 鹵2.07).Lymph node metastasis and liver metastasis were correlated with P 0.05, and with age.鎬у埆鏃犲叧(P鍧，

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