本文選題：肝細(xì)胞癌　切入點(diǎn)：圓柱瘤基因　出處：《青島大學(xué)》2017年碩士論文

【摘要】：【目的】分析圓柱瘤基因(cylindromatosis,CYLD)、高爾基體3(Golgi phosphoprotein3,GOLPH3)、核轉(zhuǎn)錄因子kappa B1(NF-κB1)和Rel A mRNA在肝細(xì)胞癌(hepatocellular carcinoma,HCC)、肝硬化、慢性肝炎患者和健康對(duì)照組外周血中的表達(dá)水平及其與HCC患者臨床特征的關(guān)系;利用受式者工作特性曲線(ROC)分析四種癌基因mRNA表達(dá)水平對(duì)HCC早期診斷的價(jià)值�！痉椒ā恳�2016年1月至2016年10月青島大學(xué)醫(yī)學(xué)院附屬醫(yī)院就診HCC患者90例、肝硬化患者41例、慢性肝炎患者46例以及健康體檢者80例作為研究對(duì)象。利用RT-PCR檢測(cè)四組研究對(duì)象外周血CYLD、GOLPH3、NF-κB1和Rel A mRNA的相對(duì)表達(dá)水平;全自動(dòng)生化儀檢測(cè)血清總膽紅素(serum total bilirubin,STB)、谷草轉(zhuǎn)氨酶(aspartate aminotransferase,AST)和谷丙轉(zhuǎn)氨酶(alanine aminotransferase,ALT)。受試者工作特征曲線(receiver operating characteristic,ROC)分析AFP、CYLD、GOLPH3、NF-κB1和Rel A mRNA對(duì)肝細(xì)胞癌的早期診斷價(jià)值�！窘Y(jié)果】HCC組外周血中CYLD mRNA的相對(duì)表達(dá)量顯著低于其它三組,其中肝硬化組明顯高于正常組和肝炎組,GOLPH3、NF-ΚB1和Rel A mRNA在HCC中的表達(dá)顯著高于其它三組。CYLD mRNA相對(duì)表達(dá)量在腫瘤大于等于5cm和高轉(zhuǎn)氨酶水平的患者中明顯降低(P0.05);GOLPH3 mRNA相對(duì)表達(dá)量在轉(zhuǎn)移、Ⅲ/Ⅳ期和高Ki67指數(shù)患者中呈現(xiàn)高表達(dá)(P0.05);NF-ΚB1和Rel A mRNA在Ⅲ/Ⅳ期的患者血液中高表達(dá)(P0.05),Rel A mRNA在門靜脈癌栓陽性和高Ki67指數(shù)的患者血液中高表達(dá)(P0.05)。五個(gè)指標(biāo)(血清AFP和CYLD、GOLPH3、NF-κB1以及Rel A mRNA表達(dá)水平)的單獨(dú)診斷價(jià)值分析顯示,AFP和CYLD mRNA ROC曲線下面積分別為0.828和0.847;而GOLPH3、NF-κB1和Rel A基因mRNA表達(dá)對(duì)HCC的診斷價(jià)值較低,曲線下面積分別為0.677、0.658和0.623。聯(lián)合檢測(cè)血清AFP水平和CYLD基因mRNA表達(dá),其靈敏度、特異度和曲線下面積分別是0.889、0.762和0.897,顯著高于AFP和CYLD的單獨(dú)診斷價(jià)值(P0.05)。在AFP陰性者中,CYLD仍然對(duì)肝癌有一定的診斷價(jià)值,曲線下面積是0.821,可以在一定程度上彌補(bǔ)AFP敏感性的不足�！窘Y(jié)論】1.HCC組CYLD mRNA相對(duì)表達(dá)量低于肝硬化組、肝炎組和正常組,其中CYLD mRNA相對(duì)表達(dá)量在肝硬化患者中明顯高于正常人和肝炎患者GOLPH3、NF-ΚB1和Rel A在HCC患者外周中的相對(duì)表達(dá)明顯高于其它三組。提示CYLD、GOLPH3、NF-ΚB1和Rel A與HCC密切相關(guān)。2.在腫瘤大于5cm、血清轉(zhuǎn)氨酶水平較高的HCC患者中,CYLD mRNA的相對(duì)表達(dá)量降低;GOLPH3 mRNA的相對(duì)表達(dá)量在轉(zhuǎn)移、Ⅲ/Ⅳ期和高Ki67增殖指數(shù)患者中升高;NF-ΚB1 mRNA的相對(duì)表達(dá)量在Ⅲ/Ⅳ期患者中升高;Rel A mRNA相對(duì)表達(dá)量在門靜脈癌栓陽性、Ⅲ/Ⅳ期和高Ki67增殖指數(shù)患者中升高。CYLD、GOLPH3、NF-ΚB1和Rel AmRNA可能與肝癌的預(yù)后有關(guān)。3.CYLD對(duì)HCC的早期診斷有一定的準(zhǔn)確性,AFP和CYLD的聯(lián)合診斷價(jià)值顯著高于AFP和CYLD單獨(dú)診斷的準(zhǔn)確性。在AFP陰性者中CYLD仍然具有一定的診斷價(jià)值,說明其在一定程度上可以彌補(bǔ)AFP敏感性低的不足。
[Abstract]:[objective] to analyze the expression levels of Cyd Cyd, Golgi body 3(Golgi phosphoprotein 3, nuclear transcription factor kappa B1 NF- 魏 B1 and Rel A mRNA in the peripheral blood of hepatocellular carcinoma (HCC), liver cirrhosis, chronic hepatitis and healthy controls and their relationship with the clinical characteristics of HCC patients. The value of mRNA expression of four kinds of oncogenes in the early diagnosis of HCC was analyzed by using the working characteristic curve of recipients. [methods] 90 patients with HCC and 41 patients with liver cirrhosis were admitted to the affiliated Hospital of Qingdao University Medical College from January 2016 to October 2016. 46 patients with chronic hepatitis and 80 healthy controls were used to detect the relative expression levels of CYLDG-GOLPH3NF- 魏 B1 and Rel A mRNA in peripheral blood by RT-PCR. Serum total bilirubin (STB), aspartate aminotransferase (AST), alanine aminotransferase (alt) and alanine aminotransferase (alt) were detected by automatic biochemical instrument. The diagnostic value of total LDLDPE NF- 魏 B1 and Rel A mRNA in patients with hepatocellular carcinoma was analyzed by receiver operating character roc. [results] in HCC group, the value of Rel A mRNA in early diagnosis of hepatocellular carcinoma was analyzed. The relative expression of CYLD mRNA in blood was significantly lower than that in the other three groups. The expression of Rel A mRNA and NF- KB1 in HCC in cirrhosis group was significantly higher than that in normal group and hepatitis group. The relative expression of CYLD mRNA in HCC was significantly higher than that in other three groups. The relative expression of CYLD mRNA in patients with tumor larger than 5cm and high transaminase level was significantly lower than that in normal group and hepatitis group. In patients with stage 鈪，

本文編號(hào)：1675660