本文選題：信號(hào)傳導(dǎo)及轉(zhuǎn)錄激活因子　切入點(diǎn)：甲狀腺癌　出處：《中國老年學(xué)雜志》2017年24期

【摘要】：目的觀察信號(hào)傳導(dǎo)及轉(zhuǎn)錄激活因子(STAT)3基因在老年甲狀腺癌組織中的表達(dá)及沉默STAT3基因?qū)谞钕侔┘?xì)胞的影響。方法將FTC-133細(xì)胞分為siRNA STAT3組、陰性對(duì)照組和空白對(duì)照組,RT-PCR測(cè)定FTC-133細(xì)胞STAT3、基質(zhì)金屬蛋白酶(MMP)2、MMP9 mRNA的表達(dá),Western印跡測(cè)定甲狀腺癌FTC-133細(xì)胞STAT3蛋白的表達(dá),MTT法測(cè)定甲狀腺癌FTC-133細(xì)胞的生長,Transwell法檢測(cè)FTC-133細(xì)胞的遷移能力。結(jié)果老年甲狀腺癌組織中STAT3陽性率高于癌旁組織(P0.05)。siRNA STAT3組FTC-133細(xì)胞中STAT3蛋白的表達(dá)量低于陰性對(duì)照組和空白對(duì)照組(P0.05)。siRNA STAT3組FTC-133細(xì)胞中STAT3、MMP2、MMP9 mRNA的表達(dá)量低于陰性對(duì)照組和空白對(duì)照組(P0.05)。siRNA STAT3組第1、2、3天的抑制率均明顯高于陰性對(duì)照組(P0.05)。siRNA STAT3組FTC-133細(xì)胞中穿膜細(xì)胞數(shù)低于陰性對(duì)照組和空白對(duì)照組(P0.05)。結(jié)論老年甲狀腺癌組織中STAT3呈高表達(dá),沉默STAT3基因?qū)谞钕侔┘?xì)胞株的增殖和侵襲能力有抑制作用。
[Abstract]:Objective to investigate the expression of signal transduction and transcriptional activator statin 3 gene in elderly thyroid carcinoma tissues and the effect of silencing STAT3 gene on thyroid cancer cells. Methods FTC-133 cells were divided into siRNA STAT3 group. The expression of STAT3 in FTC-133 cells was detected by RT-PCR and the expression of MMP9 mRNA in FTC-133 cells of thyroid carcinoma was detected by Western blot. The growth of FTC-133 cells in thyroid carcinoma was detected by transwell method. The expression of STAT3 protein in FTC-133 cells was detected by Western blotting. The growth of FTC-133 cells in thyroid carcinoma was detected by transwell method. Results the positive rate of STAT3 in the elderly thyroid carcinoma tissues was higher than that in the FTC-133 cells of the paracancerous tissues P0.05. siRNA STAT3 group. The expression of STAT3 protein in the FTC-133 cells was lower than that in the negative control group and the blank control group. The expression of STAT3 and MMP2MMP9 mRNA in FTC-133 cells was lower than that in the control group. The inhibition rate of FTC-133 cells in the control group and the blank control group was significantly higher than that in the negative control group (P0.05). SiRNA STAT3 group was significantly lower than that in the negative control group and the blank control group. Conclusion the expression of STAT3 in the elderly thyroid carcinoma tissues was higher than that in the negative control group and the blank control group. Silencing STAT3 gene can inhibit the proliferation and invasion of thyroid cancer cell line.
【作者單位】： 河北醫(yī)科大學(xué)附屬唐山工人醫(yī)院頭頸外科;
【分類號(hào)】：R736.1

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