本文選題：Notch3　切入點：Lgr5　出處：《遵義醫(yī)學(xué)院》2016年碩士論文

【摘要】：目的:觀察Notch3、Lgr5和NF-κB在人大腸腺癌組織、大腸腺瘤組織以及正常大腸黏膜中的表達(dá)情況,探討其與大腸腺癌臨床病理特征之間的關(guān)系。方法:本實驗選擇54例大腸腺癌組織、30例大腸腺瘤組織及12例正常大腸黏膜組織,采用免疫組織化學(xué)染色SP法檢測各組織中Notch3、Lgr5、NF-κB蛋白的表達(dá)情況。結(jié)合大腸腺癌患者的臨床病理資料,比較各組組織中三種蛋白因子的表達(dá)與臨床病理特征的相關(guān)性。結(jié)果:Notch3在大腸腺癌、大腸腺瘤、正常大腸黏膜中的表達(dá)陽性率分別為63.0%、26.7%、8.3%,癌組與腺瘤組及正常組相比,差異均具有統(tǒng)計學(xué)意義(P0.05),腺瘤組與正常組相比,差異無統(tǒng)計學(xué)意義(P0.05);Lgr5在大腸腺癌、大腸腺瘤、正常大腸黏膜中的表達(dá)陽性率分別為57.4%、36.7%、16.7%,癌組與腺瘤組及正常組相比,差異均具有統(tǒng)計學(xué)意義(P0.05),腺瘤組與正常組相比,差異無統(tǒng)計學(xué)意義(P0.05);NF-κB在大腸腺癌、大腸腺瘤、正常大腸黏膜中的表達(dá)陽性率分別為63.0%、30.0%、25.0%,癌組與腺瘤組及正常組相比,差異均具有統(tǒng)計學(xué)意義(P0.05),腺瘤組與正常組相比,差異無統(tǒng)計學(xué)意義(P0.05)。Notch3、Lgr5、NF-κB在大腸腺癌中的表達(dá)均與性別、腫瘤大小及分化程度無關(guān)(P0.05),而均與淋巴結(jié)轉(zhuǎn)移及Dukes分期相關(guān)(P0.05),伴有淋巴結(jié)轉(zhuǎn)移者表達(dá)強(qiáng)度高于無淋巴結(jié)轉(zhuǎn)移者(P0.05),Dukes分期級別越高,其表達(dá)程度越高(P0.05)。此外,Notch3的表達(dá)與年齡無關(guān)(P0.05),而與腫瘤浸潤深度有關(guān),腫瘤浸潤深度越大,表達(dá)強(qiáng)度越強(qiáng)(P0.05);Lgr5與年齡及腫瘤浸潤深度無關(guān)(P0.05);NF-κB與腫瘤原發(fā)部位無關(guān)(P0.05),而與腫瘤浸潤深度有關(guān),腫瘤浸潤深度越大,表達(dá)越強(qiáng)(P0.05);大腸腺癌中Notch3與Lgr5的表達(dá)呈正相關(guān)(r=0.325,P0.05),Notch3與NF-κB表達(dá)無線性相關(guān)(P0.05),Lgr5與NF-κB表達(dá)無線性相關(guān)(P0.05)。結(jié)論:1.Notch3、Lgr5、NF-κB在正常大腸黏膜、大腸腺瘤及大腸腺癌組織中的表達(dá)呈逐漸增高趨勢,三者可能與大腸腺癌的發(fā)生具有一定的關(guān)系。2.Notch3、Lgr5、NF-κB三者的表達(dá)與大腸腺癌的Dukes分期及有無淋巴結(jié)轉(zhuǎn)移顯著相關(guān),而且Notch3、NF-κB兩者還與大腸腺癌的浸潤深度顯著相關(guān),因此,上述三個指標(biāo)可在一定程度上預(yù)測大腸腺癌的惡性程度及判斷患者預(yù)后,其三者的高表達(dá)也可以作為大腸腺癌淋巴結(jié)轉(zhuǎn)移、Dukes分期的重要生物學(xué)指標(biāo)。3.Notch3、Lgr5在大腸腺癌中的表達(dá)呈正相關(guān)。
[Abstract]:Objective: to observe the expression of Notch3G Lgr5 and NF- 魏 B in human colorectal adenocarcinoma, colorectal adenoma and normal colorectal mucosa. Methods: 54 cases of colorectal adenocarcinoma, 30 cases of colorectal adenoma and 12 cases of normal colorectal mucosa were selected. Immunohistochemical staining SP method was used to detect the expression of Notch3ONg5NF-kappa B protein in various tissues, and combined with clinicopathological data of colorectal adenocarcinoma patients, the expression of NF- 魏 B protein was detected by immunohistochemical staining. Results the positive rates of the expression of the three protein factors in colorectal adenocarcinoma, colorectal adenoma and normal colorectal mucosa were 63.0 and 26.78.3.The positive rate of the tumor group was higher than that of the adenoma group and the normal mucosa. There was no significant difference between adenoma group and normal group. The positive rates of Lgr5 expression in colorectal adenocarcinoma, adenoma and normal mucosa were 57.4% and 36.7%, respectively. The positive rates of NF- 魏 B in adenoma group, adenoma group and normal mucosa were 63.0% and 30.0% respectively. The positive rate of NF- 魏 B in adenoma group was higher than that in adenoma group and normal group, and the positive rates of NF- 魏 B in adenoma group and normal mucosa were 63.0% and 25.0% respectively, and the positive rate of NF- 魏 B in adenoma group was significantly higher than that in normal group. There was no significant difference in the expression of NF- 魏 B between adenoma group and normal group. The tumor size and differentiation degree were not correlated with lymph node metastasis and Dukes stage. The expression intensity of tumor with lymph node metastasis was higher than that without lymph node metastasis. In addition, the expression of Notch3 was not related to age, but was related to the depth of tumor invasion. The stronger the expression intensity was, the more P0.05 Lgr5 was not related to age and depth of tumor invasion. NF- 魏 B was not related to the primary site of the tumor, but was related to the depth of tumor invasion. The expression of Notch3 and Lgr5 in colorectal adenocarcinoma was positively correlated with the expression of NF- 魏 B and the expression of P0.05, Lgr5 and NF- 魏 B.Conclusion the expression of Notch3 and NF- 魏 B in normal colorectal mucosa, colorectal adenoma and adenocarcinoma of large intestine showed a tendency to increase gradually, and the expression of Lgr5NF- 魏 B in normal colorectal mucosa, colorectal adenoma and adenocarcinoma of large intestine showed a tendency of increasing gradually, and the expression of P0.05 and NF- 魏 B in colorectal adenocarcinoma was increased gradually, conclusion: 1.Notch3Lgr5NF- 魏 B in normal colorectal mucosa, colorectal adenoma and adenocarcinoma of large intestine showed an increasing tendency. 2. The expression of NF- 魏 B was significantly correlated with the Dukes stage and lymph node metastasis of colorectal adenocarcinoma, and Notch3NF- 魏 B was significantly correlated with the depth of invasion of colorectal adenocarcinoma. The above three indexes can predict the malignancy of colorectal adenocarcinoma to some extent and judge the prognosis of the patients. The high expression of these three proteins can also be regarded as an important biological index of Dukes staging in colorectal adenocarcinoma. 3. The expression of Notch3Lgr5 in colorectal adenocarcinoma is positively correlated.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R735.34

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