本文選題：微小RNA-23a　切入點：外周血　出處：《浙江大學》2015年碩士論文

【摘要】：背景及目的 胃癌是危害我國人民身體健康的重大疾病。胃癌防控的關(guān)鍵在于早期診斷和早期治療。目前胃癌的篩查和早期診斷依賴胃鏡檢查,費用高,患者接受度低。缺乏用于胃癌診斷外周血分子標志物。本實驗室前期關(guān)于外周血miRNA高通量測序的研究發(fā)現(xiàn)多個在胃癌患者中和健康人群中差異表達的miRNA,包其中括miR-23a.本研究旨在不同人群中收集外周血,分析其miR-23a表達量,以期發(fā)現(xiàn)其表達量與胃癌的關(guān)系以及與臨床和病理特征的相關(guān)性,進而評估其是否有成為胃癌外周血檢測分子標志物的可能性。 方法 在通過醫(yī)院倫理委員會批準以及患者知情同意的情況下收集邵逸夫醫(yī)院就診的患者外周血及臨床資料,采用高速離心的方式獲取血清,后通過實時熒光定量逆轉(zhuǎn)錄PCR的方法檢測血清中miR-23a表達量,結(jié)合檢測結(jié)果及臨床資料,采用獨立樣本的t檢驗、卡方檢驗及spearman相關(guān)性分析其與胃癌的相關(guān)性。 結(jié)果 1)胃癌組外周血miR-23a表達量較健康對照組顯著升高,差異具有統(tǒng)計學意義。 2)以外周血miR-23a表達量ACT值12.335為陽性判斷標準時,對于胃癌的判斷靈敏度為63%,特異度為60%。胃癌組中陽性患者淋巴結(jié)轉(zhuǎn)移率較高,與陰性者比較有統(tǒng)計學意義。 3)在胃癌患者中外周血1niR-23a表達量與印戒細胞癌呈負相關(guān),及腫瘤的分化程度呈正相關(guān)。 結(jié)論 外周血miR-23a作為胃癌診斷的外周血分子標志物可能有有助于胃癌患者預(yù)后的判斷。
[Abstract]:Background and purpose. Gastric cancer is a major disease that endangers the health of our people. The key to the prevention and control of gastric cancer lies in early diagnosis and treatment. At present, the screening and early diagnosis of gastric cancer depends on gastroscopy and the cost is high. Low acceptance of patients. Lack of molecular markers for the diagnosis of gastric cancer peripheral blood. Our previous study of peripheral blood miRNA high-throughput sequencing found a number of differentially expressed miRNAs in patients with gastric cancer and in healthy people, including. The aim of this study was to collect peripheral blood from different populations. The expression of miR-23a was analyzed in order to find out the relationship between the expression of miR-23a and gastric cancer and its correlation with clinical and pathological features, and to evaluate the possibility of its expression as a molecular marker in peripheral blood detection of gastric cancer. Method. With the approval of the Hospital Ethics Committee and the informed consent of the patients, the peripheral blood and clinical data of the patients treated by Shaw Hospital were collected, and the serum was obtained by high-speed centrifugation. The expression of miR-23a in serum was detected by real-time fluorescence quantitative reverse transcription (PCR). The correlation between miR-23a and gastric cancer was analyzed by t-test of independent samples, chi-square test and spearman correlation analysis. Results. 1) the expression of miR-23a in peripheral blood of gastric cancer group was significantly higher than that of healthy control group. 2) when the ACT value of miR-23a expression in peripheral blood was taken as the positive criterion, the sensitivity and specificity for gastric cancer were 63 and 600.The lymph node metastasis rate of the positive patients was higher than that of the negative patients. 3) in gastric cancer patients, the expression of 1niR-23a in peripheral blood was negatively correlated with signet ring cell carcinoma and positively correlated with the degree of tumor differentiation. Conclusion. Peripheral blood miR-23a as a molecular marker in the diagnosis of gastric cancer may be helpful to the prognosis of gastric cancer patients.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R735.2

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相關(guān)期刊論文 前1條

1 金愛紅;周霞平;周鳳珍;;抑制miR-23a表達增強卵巢癌順鉑敏感性的分子機制[J];南方醫(yī)科大學學報;2015年01期

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本文編號：1663211