本文選題：亞硒酸鈉　切入點：肺癌　出處：《營養(yǎng)學報》2017年02期 　論文類型：期刊論文

【摘要】：目的觀察亞硒酸鈉對肺癌細胞株A549細胞株自噬相關(guān)蛋白Beclin1、LC3的作用。方法將人肺癌A549細胞株分為0、0.5、2.5、5.0、8.0μmol/L亞硒酸鈉共5組,用不同濃度的亞硒酸鈉培養(yǎng)液培養(yǎng)24h、48h后,用免疫細胞化學染色和Western blot印跡法檢測亞硒酸鈉對自噬相關(guān)蛋白Beclin1、LC3表達的影響。結(jié)果人肺癌A549細胞株用不同濃度的亞硒酸鈉培養(yǎng)24h,48h后,細胞數(shù)量逐漸減少,細胞腫脹,變大變圓,染色質(zhì)凝集、核固縮并斷裂。免疫細胞化學和Western blot印跡法結(jié)果均顯示:隨著亞硒酸鈉濃度增加,肺癌A549細胞中Beclin1和LC3蛋白免疫細胞化學染色的平均光密度(MOD)有明顯提高(P0.05)。結(jié)論亞硒酸鈉可以抑制肺癌A549細胞生長,誘導肺癌A549細胞自噬。
[Abstract]:Objective to observe the effect of sodium selenite on autophagy associated protein Beclin1 (LC3) in human lung cancer cell line A549. Methods Human lung cancer A549 cell line was divided into 5 groups. The cells were cultured with different concentrations of sodium selenite for 24 h or 48 h. The effect of sodium selenite on the expression of Beclin1 hLC3 was detected by immunocytochemical staining and Western blot blotting. Results after cultured with different concentrations of sodium selenite for 48 h, the number of A549 cells gradually decreased and the cells became swollen. The results of immunocytochemistry and Western blot blotting showed that: with the increase of sodium selenite concentration, The average optical density (mod) of Beclin1 and LC3 protein immunocytochemical staining in lung cancer A549 cells increased significantly. Conclusion Sodium selenite can inhibit the growth of lung cancer A549 cells and induce autophagy of lung cancer A549 cells.
【作者單位】： 泰山醫(yī)學院組胚教研室;
【基金】：國家自然科學基金(No.81572868) 山東省自然科學基金(No.ZR2011CM042);山東省自然科學基金(No.ZR2014CM023)
【分類號】：R734.2

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