本文選題：前列腺癌　切入點：FISH　出處：《青島大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過FISH檢測C-myc和PSCA在前列腺癌和前列腺增生組織中的表達情況,分析C-myc和PSCA在前列癌組織和前列腺增生組織中的表達情況以及與前列腺癌的臨床分期的關(guān)系,探討C-myc和PSCA在早期前列腺癌診斷中的應(yīng)用。方法:選擇2013年12月-2016年10月昌樂縣人民醫(yī)院泌尿外科前列腺患者病理標本55例,其中前列腺癌標本為25例,前列腺增生標本30例。患者術(shù)前未經(jīng)過任何的放化療。利用FISH方法檢測55例前列腺組織中的C-myc和PSCA的表達情況。使用SPSS16.0軟件進行Pearson相關(guān)性分析,研究二者的表達與前列腺癌的診斷、臨床分期之間的關(guān)系。結(jié)果:C-myc基因的陽性表達率與前列腺細胞學(xué)及組織學(xué)的病變程度呈現(xiàn)出正相關(guān)(P0.001),隨著前列腺細胞學(xué)及組織學(xué)的惡變程度加重而有明顯增加(P0.001)。PSCA的表達在前列腺癌組和前列腺增生組中的陽性表達率并沒有明顯的差異(P0.05),PSCA在前列腺癌和前列腺增生組中的強陽性表達率,二者之間的差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1、C-myc基因與前列腺病變程度呈現(xiàn)出正相關(guān)關(guān)系,可作為預(yù)測前列腺病變發(fā)展的重要指標,可作為早期前列腺癌和高度可疑前列腺癌病變的指標。2、PSCA強陽性表達率在前列腺癌組中明顯高于前列腺增生組,并且前列腺癌組中的PSCA表達水平上臨床分期有相對較顯著的相關(guān)性,提示出PSCA對前列腺癌診斷及預(yù)后判斷有相當?shù)膬r值。3、結(jié)合C-myc基因與PSCA對前列腺癌診斷可增加對于診察判斷的敏銳度。
[Abstract]:Objective: to detect the expression of C-myc and PSCA in prostate cancer and prostatic hyperplasia by FISH, and to analyze the expression of C-myc and PSCA in prostatic carcinoma and prostatic hyperplasia, and the relationship between C-myc and PSCA and the clinical stage of prostate cancer. To explore the application of C-myc and PSCA in the diagnosis of early prostate cancer. Methods: from December 2013 to October 2016, 55 patients with prostate cancer were selected from the urological department of Changle people's Hospital, including 25 patients with prostate cancer. The expression of C-myc and PSCA in 55 cases of prostatic tissue was detected by FISH method. The correlation analysis of Pearson was performed with SPSS16.0 software. To study the expression of both and the diagnosis of prostate cancer, Results there was a positive correlation between the positive expression rate of C myc gene and the pathological changes of prostate cytology and histology (P 0.001). The positive expression rate of C myc gene was significantly increased with the degree of malignancy of prostate cytology and histology. There was no significant difference in the positive expression rate of P0.05 and PSCA between prostate cancer and prostatic hyperplasia, and there was no significant difference in the positive expression rate of P0.05 and PSCA between prostate cancer and benign prostatic hyperplasia. Conclusion there is a positive correlation between the C-myc gene and the degree of prostatic disease, which can be used as an important index to predict the development of prostatic disease. The positive rate of PSCA in prostate cancer group was significantly higher than that in prostatic hyperplasia group. There was a relatively significant correlation between the clinical stage and the expression of PSCA in prostate cancer. It is suggested that PSCA has a significant value in the diagnosis and prognosis of prostate cancer. Combination of C-myc gene and PSCA can increase the sensitivity of diagnosis and judgment of prostate cancer.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.25

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