本文選題：胃癌　切入點(diǎn)：miRNA-449　出處：《皖南醫(yī)學(xué)院》2015年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的：通過(guò)對(duì)miRNA-449基因和E2F1蛋白在胃癌組織中表達(dá)的研究,并分析miRNA-449的表達(dá)與臨床資料的關(guān)系,探討其在胃癌發(fā)生中的作用機(jī)制,為胃癌早期診斷、有效治療和預(yù)后評(píng)估提供依據(jù)。方法：將收集到的31對(duì)胃癌及癌旁組織標(biāo)本,提取組織總RNA,莖環(huán)法反轉(zhuǎn)錄成cDNA,實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)niRNA-449基因表達(dá)水平；提取總蛋白,Western Blot方法檢測(cè)E2F1蛋白表達(dá)水平。結(jié)果1.miRNA-449的表達(dá)：miRNA-449a在31例胃癌中相對(duì)于癌旁組織有23例(74.19%)表達(dá)下調(diào),8例(25.81%)表達(dá)上調(diào)。miRNA-449a的相對(duì)表達(dá)水平：胃癌組為3.85±2.45,癌旁組為4.66±2.41,兩者相比,miRNA-449a在胃癌中的表達(dá)低于癌旁組織,差異有統(tǒng)計(jì)學(xué)意義(配對(duì)t=2.516,P=0.017,P0.05)。miRNA-449b在31例胃癌中相對(duì)于癌旁組織有24例(77.42%)表達(dá)下調(diào),7例(22.58%)表達(dá)上調(diào)。miRNA-449b的相對(duì)表達(dá)水平：胃癌組為3.66±2.54,癌旁組為4.27±2.33,兩者相比,miRNA-449b在胃癌中的表達(dá)低于癌旁組織,差異有統(tǒng)計(jì)學(xué)意義(配對(duì)t=2.525,P=0.017,P0.05)。其中miRNA-449a和miRNA-449b在胃癌組織中有21例表達(dá)一致下調(diào),6例表達(dá)一致上調(diào)。niRNA-449c在31例胃癌中相對(duì)于癌旁非腫瘤組織有18例(58.06%)表達(dá)下調(diào),13例(41.94%)表達(dá)上調(diào)。miRNA-449c的相對(duì)表達(dá)水平：胃癌組為2.82±1.98,癌旁組為3.05-2.05,兩者相比,miRNA-449c在胃癌組織與癌旁組織中的平均表達(dá)無(wú)統(tǒng)計(jì)學(xué)意義(配對(duì)t=0.679, P=0.503, P0.05). miRNA-449a/b/c在胃腺癌細(xì)胞株(BGC-823)中的表達(dá)均低于正常胃黏膜細(xì)胞株(GES-1) (P=0.003/0.017/0.005, P0.05)。2. miRNA-449a/b的表達(dá)與臨床資料的關(guān)系：miRNA-449a/b的表達(dá)變化與淋巴結(jié)轉(zhuǎn)移顯著相關(guān)。miRNA-449a/b在18例有淋巴結(jié)轉(zhuǎn)移的樣本中均有17例表達(dá)下調(diào)(94.45%),13例無(wú)淋巴結(jié)轉(zhuǎn)移的樣本分別有6例(46.15%)和7例表達(dá)下調(diào)(53.85%),淋巴結(jié)轉(zhuǎn)移組與無(wú)淋巴結(jié)轉(zhuǎn)移組miRNA-449 a/b的下調(diào)比例,經(jīng)Fisher確切概率法檢驗(yàn),差異有統(tǒng)計(jì)學(xué)意義(P=0.004/0.012,P0.05)。而miRNA-449a/b在胃癌中的表達(dá)變化與患者的年齡、性別、腫瘤的直徑、腫瘤的浸潤(rùn)深度以及分化程度等無(wú)相關(guān)性。3.E2F1蛋白的表達(dá)：E2F1在17例胃癌中相對(duì)于癌旁組織有13例(76.47%)表達(dá)上調(diào),4例(23.53%)表達(dá)下調(diào)。E2F1的相對(duì)表達(dá)水平：胃癌組為1.14±0.49,癌旁組為0.97±0.41,兩者相比,E2F1在胃癌中的表達(dá)高于癌旁組織,差異有統(tǒng)計(jì)學(xué)意義(配對(duì)t=2.138,P=0.048,P0.05)。結(jié)論：1.在胃癌中,存在niRNA-449a/b基因的低表達(dá)和E2F1蛋白的高表達(dá),提示miRNA-449a/b口E2F1與胃癌的發(fā)生相關(guān)。2. miRNA-449a/b在胃癌中的表達(dá)變化與淋巴結(jié)轉(zhuǎn)移相關(guān),提示miRNA-449a/b可能作為判別胃癌預(yù)后的指標(biāo)之一。
[Abstract]:Objective: to study the expression of miRNA-449 gene and E2F1 protein in gastric carcinoma, and to analyze the relationship between the expression of miRNA-449 and clinical data, and to explore the mechanism of miRNA-449 expression in gastric carcinogenesis, so as to provide early diagnosis of gastric cancer. Methods: the total RNAs were extracted from 31 samples of gastric cancer and adjacent tissues, and then reversely transcribed into cDNAs by stem loop method. The expression level of niRNA-449 gene was detected by real-time fluorescence quantitative PCR. Results 1. The expression of miRNA-449 in 31 cases of gastric cancer was down-regulated in 23 cases (74.19%) of 31 cases of gastric cancer compared with adjacent tissues.) the expression of miRNA-449a was up-regulated in 8 cases (25.81%): the expression level of miRNA-449a was 3.85 鹵2.45 in gastric cancer group and 3.85 鹵2.45 in adjacent gastric carcinoma group. The expression of miRNA-449a in gastric cancer was lower than that in paracancerous tissues. The down-regulated expression of miRNA-449b in 31 cases of gastric cancer was significantly lower than that of adjacent tissues (77.42%).) the expression level of miRNA-449b in gastric cancer group was 3.66 鹵2.54 and that in paracancerous group was 4.27 鹵2.33. The expression of miRNA-449b in gastric cancer group was 3.66 鹵2.54 and that in paracancerous group was 4.27 鹵2.33. The expression of miRNA-449b in gastric cancer was higher than that in para-cancerous tissue. Below the surrounding tissue, The expression of miRNA-449a and miRNA-449b were down-regulated in 21 cases of gastric carcinoma and 6 cases were up-regulated. NiRNA-449c was down-regulated in 13 cases (41.94%) in 31 cases of gastric cancer as compared with 18 cases of non-cancerous tissues adjacent to cancer. Upregulation of the relative expression level of .miRNA-449c: 2.82 鹵1.98 in gastric cancer group and 3.05-2.05 in paracancerous group. There was no significant difference in the average expression of miRNA-449c between gastric cancer tissue and paracancerous tissue (pairwise t0.679, P0. 503, P0. 05). The expression of miRNA-449a/b/c in gastric adenocarcinoma cell line BGC-823 was lower than that in gastric cancer cell line BGC-823. Normal gastric mucosal cell line (GES-1) P0. 003 / 0.017 / 0.005, P0. 05 / 0.2.The relationship between the expression of miRNA-449a/b and clinical data is significant correlation between the expression of miRNA-449a / b and lymph node metastasis. MiRNA-449a / b is down-regulated in 17 out of 18 samples with lymph node metastasis, and there are 13 cases without lymph node metastasis. The down-regulation of miRNA-449 a / b in lymph node metastasis group and non-lymph node metastasis group was 53.85% and 53.85% respectively, and the down-regulation ratio of miRNA-449 a / b in lymph node metastasis group and non-lymph node metastasis group was lower than that in non-lymph node metastasis group. By Fisher's exact probability test, the difference was statistically significant. The expression of miRNA-449a/b in gastric cancer was correlated with the age, sex and diameter of the tumor. (3. Expression of E2F1 protein in 17 cases of gastric carcinoma: 13 cases in relation to adjacent tissues.) upregulation and down-regulation of expression of E2F1: 1.14 鹵0.49 in gastric cancer group and 23.53% in paracancerous tissue. The expression of E2F1 in gastric cancer was higher than that in paracancerous tissues. Conclusion: 1. There is a low expression of niRNA-449a/b gene and a high expression of E2F1 protein in gastric carcinoma, suggesting that the expression of E2F1 in miRNA-449a/b mouth is related to carcinogenesis of gastric cancer .2.2.The expression of miRNA-449a/b in gastric carcinoma is related to lymph node metastasis. The results suggest that miRNA-449a/b may be one of the predictors of gastric cancer prognosis.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R735.2

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本文編號(hào)：1637155