本文選題：-氯腺苷　切入點(diǎn)：乳腺癌　出處：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2017年11期 　論文類型：期刊論文

【摘要】：目的:研究8-氯腺苷(8-chloro-adenosine,8-Cl-Ado)對(duì)乳腺癌SK-BR-3細(xì)胞增殖和遷移的影響,以及與RNA編輯酶1(adenosine deaminases acting on RNA 1,ADAR1)表達(dá)的相關(guān)性。方法:蛋白印跡實(shí)驗(yàn)檢測(cè)乳腺癌組織中ADAR1的蛋白表達(dá)量以及8-Cl-Ado(10μmol/L)處理乳腺癌SK-BR-3細(xì)胞不同時(shí)間ADAR1蛋白表達(dá)量的變化;MTT法和形態(tài)學(xué)觀察檢測(cè)不加藥組和8-Cl-Ado(10μmol/L)加藥組對(duì)SK-BR-3細(xì)胞增殖的影響;MTT法和傷口愈合實(shí)驗(yàn)檢測(cè)SK-BR-3細(xì)胞過(guò)表達(dá)ADAR1質(zhì)粒(空白對(duì)照組、空載組、ADAR1-P110組、ADAR1-P150組)對(duì)8-Cl-Ado作用SK-BR-3細(xì)胞增殖及遷移的影響。結(jié)果:乳腺癌癌組織中ADAR1-P110和ADAR1-P150蛋白表達(dá)量均明顯高于癌旁組織(t=9.871,P=0.000;t=7.169,P=0.000);8-Cl-Ado作用SK-BR-3細(xì)胞48 h后ADAR1-P110和ADAR1-P150蛋白表達(dá)量均明顯降低(t=-8.838,P=0.013;t=-19.866,P=0.003);8-Cl-Ado作用SK-BR-3細(xì)胞ADAR1-P110組和ADAR1-P150組48 h后增殖抑制率均明顯低于空白組(P均為0.000);8-Cl-Ado作用SK-BR-3細(xì)胞ADAR1-P110組和ADAR1-P150組48 h后的遷移率均明顯高于空白組(P均為0.000)。結(jié)論:8-Cl-Ado能明顯抑制SK-BR-3細(xì)胞增殖和遷移,其作用機(jī)制可能與ADAR1的表達(dá)下調(diào)有關(guān)。
[Abstract]:Objective: to study the effects of 8-chloro-adenosine 8-chloro-adenosine 8-Cl-Ado) on the proliferation and migration of breast cancer SK-BR-3 cells. Methods: Western blot assay was used to detect the expression of ADAR1 protein in breast cancer tissues and the changes of ADAR1 protein expression in breast cancer SK-BR-3 cells treated with 8-Cl-Adol 10 渭 mol / L at different times. And morphological observation to detect the effect of adding 8-Cl-Adol 10 渭 mol / L on the proliferation of SK-BR-3 cells and the wound healing test to detect the overexpression of ADAR1 plasmid in SK-BR-3 cells (blank control group). The effect of ADAR1-P110 group on proliferation and migration of SK-BR-3 cells induced by 8-Cl-Ado. Results: the expression of ADAR1-P110 and ADAR1-P150 protein in breast cancer tissues was significantly higher than that in adjacent tissues of SK-BR-3 cells, ADAR1-P110 and ADAR1-P150 protein expression were significantly higher than those in the adjacent tissues of SK-BR-3 cells treated with 8-Cl-Ado for 48 h after treatment with ADAR1-P110 group (ADAR1-P110). The inhibition rate of proliferation in ADAR1-P110 group and ADAR1-P150 group after 48 h was significantly lower than that in ADAR1-P110 group and ADAR1-P150 group (P < 0. 000). The migration rate of SK-BR-3 cells in ADAR1-P110 group and ADAR1-P150 group was significantly higher than that in control group (P = 0. 000). Conclusion the inhibition rate of proliferation in ADAR1-P110 group and ADAR1-P150 group is significantly lower than that in ADAR1-P110 group and ADAR1-P150 group after 48 h. Conclusion: 8-Cl-Ado can significantly inhibit the proliferation of SK-BR-3 cells in ADAR1-P110 group and ADAR1-P150 group after 48 hours of treatment. Conclusion: 8-Cl-Ado can significantly inhibit the proliferation of SK-BR-3 cells in ADAR1-P110 group and ADAR1-P150 group after 48 h. Conclusion:% 8-Cl-Ado can significantly inhibit the proliferation of SK-BR-3 cells. Proliferation and migration of SK-BR-3 cells, Its mechanism may be related to the down-regulation of ADAR1 expression.
【作者單位】： 重慶醫(yī)科大學(xué)分子醫(yī)學(xué)與腫瘤研究中心;
【分類號(hào)】：R737.9
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本文編號(hào)：1628262