天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

當前位置:主頁 > 醫(yī)學論文 > 腫瘤論文 >

MicroRNA相關基因DROSHA、DICER1、GEMIN4單核苷酸多態(tài)性與T細胞淋巴瘤患者預后關系

發(fā)布時間:2018-03-16 21:19

  本文選題:MicroRNA 切入點:單核苷酸多態(tài)性 出處:《第三軍醫(yī)大學》2017年碩士論文 論文類型:學位論文


【摘要】:背景:T細胞淋巴瘤(T-cell lymphoma,TCL)是起源成熟或者胸腺后T淋巴細胞以及自然殺傷(NK)細胞的淋巴造血系統(tǒng)腫瘤,異質性高,大部分T細胞淋巴瘤惡性程度較B細胞淋巴瘤高,預后差。我國TCL發(fā)病率顯著高于西方,大概占淋巴瘤的25%。國內(nèi)外,臨床上各種類型淋巴瘤在國際預后指數(shù)(international prognostic index,IPI)的基礎上,結合各自的特點、綜合其他預后因素形成各自不同的IPI,以此評價患者的預后,但此評分標準尚不能完全確切的評判所有TCL患者的預后情況。小RNA(Micro RNA,miRNA)是一類由大約21-24個核苷酸組成、高度保守的、內(nèi)源性非編碼小RNA,參與基因轉錄后調(diào)控,F(xiàn)已有文獻報道m(xù)i RNA與癌癥的發(fā)生、發(fā)展相關。在人類基因組的遺傳變異中,單核苷酸多態(tài)性(Single nucleotide polymorphism,SNP)是最常見的一種形式,而miRNA相關基因的多態(tài)性與mi RNA的合成、下游生物學作用的發(fā)揮有關。目前有關mi RNA相關基因多態(tài)性與TCL患者預后關聯(lián)的報道很少。目的:探討microRNA相關基因DROSHA rs10719和rs6877842、DICER1 rs3742330和GEMIN4 rs3744741單核苷酸多態(tài)性與T細胞淋巴瘤患者預后的關系。材料和方法:運用聚合酶鏈式反應-限制性片段長度多態(tài)(PCR-RFLP)技術,對本研究共收集的163個TCL患者外周血標本進行分析,先后檢測4個SNP的基因型,最后綜合臨床相關數(shù)據(jù)探討這4個SNP與患者完全緩解(complete remission,CR)率和總體生存(overall survival,OS)率的相關性。結果:臨床特征與TCL患者CR率和OS率的相關性分析結果顯示:病理類型、腫瘤分期、血清乳酸脫氫酶(lactate dehydrogenase,LDH)水平及IPI評分與CR率和OS率顯著相關。Logistic-Regression分析結果顯示rs6877842和rs3742330是TCL患者CR率有關的獨立預后因素。在rs6877842的CG、CC兩種基因型中,攜帶CG基因型的患者CR率明顯優(yōu)于攜帶CC基因型的。在rs3742330的GG、GA、AA三種基因型中,攜帶GG基因型的患者CR率顯著優(yōu)于攜帶GA基因型或AA基因型的患者。多因素Cox模型分析結果顯示rs3742330是與TCL患者OS有關聯(lián)的獨立預后因素,其中攜帶GG基因型的患者OS率明顯高于攜帶GA基因型或AA基因型的患者。而rs10719和rs3744741與CR率或OS率均無明顯相關性。結論:DROSHA rs6877842和DICER1 rs3742330是TCL患者CR率相關的獨立預后因素;DICER1 rs3742330也是TCL患者OS率相關的獨立預后因素。有利于預測TCL患者的緩解率和生存時間。
[Abstract]:Background: the T-cell lymphomatosis (TCLs) is a tumor of lymphatic hematopoietic system of mature or retrothymic T lymphocytes and natural killer NKK cells with high heterogeneity. Most T-cell lymphomas are more malignant than B-cell lymphomas. The prognosis is poor. The incidence of TCL in China is significantly higher than that in the West, accounting for about 25% of the lymphoma. At home and abroad, various types of lymphoma are based on the international prognostic index and their respective characteristics. Other prognostic factors are taken into account to evaluate the prognosis of patients with TCL. However, this score does not exactly determine the prognosis of all patients with TCL. Small RNAs miRNAs are composed of about 21-24 nucleotides and are highly conserved. Endogenous non-coding small RNAs are involved in gene posttranscriptional regulation. It has been reported that mi RNA is associated with the occurrence and development of cancer. In the genetic variation of the human genome, single nucleotide polymorphismNPs are the most common form. The polymorphism of miRNA related gene and the synthesis of miRNA, There are few reports about the association between the polymorphism of mi RNA associated gene and prognosis of TCL patients. Objective: to investigate the single nucleotide polymorphisms of DROSHA rs10719 and rs6877842 DICER1 rs3742330 and GEMIN4 rs3744741 in patients with TCL. Materials and methods: polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used. The peripheral blood samples collected from 163 patients with TCL were analyzed and 4 genotypes of SNP were detected. Finally, the correlation between the four SNP and the complete remission rate of complete remission and the overall survival rate of patients with TCL was discussed. Results: the correlation analysis between clinical features and CR rate and OS rate of TCL patients showed that: pathological type, tumor stage, tumor stage. Serum lactate dehydrogenase level and IPI score were significantly correlated with CR rate and OS rate. The results showed that rs6877842 and rs3742330 were independent prognostic factors related to CR rate in TCL patients. The CR rate of patients with CG genotype was significantly higher than that with CC genotype. The CR rate of patients with GG genotype was significantly higher than that of patients with GA genotype or AA genotype. Multivariate Cox model analysis showed that rs3742330 was an independent prognostic factor associated with OS in TCL patients. The OS rate of patients with GG genotype was significantly higher than that of patients with GA genotype or AA genotype, while rs10719 and rs3744741 had no significant correlation with CR rate or OS rate. Conclusion\\\%\\%\%\% drop\%\%\%\%\%\%\%\%\%. DICER1 rs3742330 is also an independent prognostic factor related to OS rate in TCL patients, which is helpful to predict remission rate and survival time of TCL patients.
【學位授予單位】:第三軍醫(yī)大學
【學位級別】:碩士
【學位授予年份】:2017
【分類號】:R733.1

【參考文獻】

相關期刊論文 前6條

1 周穎;張松松;趙瑜;馬超;陸曉林;劉婷;于力;;造血干細胞移植治療外周T細胞淋巴瘤療效及預后分析[J];中國實驗血液學雜志;2015年03期

2 武莉麗;刁蘭萍;高玉環(huán);李濤;劉麗宏;劉海生;趙桂敏;;MMP-2和TIMP-2基因啟動子區(qū)多態(tài)性與侵襲性T細胞淋巴瘤關系的研究[J];中國現(xiàn)代醫(yī)學雜志;2013年35期

3 張松松;魏敏;李紅華;靖_g;李菲;黃文榮;陸曉林;劉占祥;周穎;于力;;47例結外NK/T細胞淋巴瘤治療和預后分析[J];中國實驗血液學雜志;2011年01期

4 侯軍;章衛(wèi)平;邱慧穎;鄭曉麗;王利平;倪雄;宋獻民;王健民;;自體外周血干細胞移植治療T細胞淋巴瘤的臨床研究[J];臨床腫瘤學雜志;2009年02期

5 黃曉春;李曉林;黃然欣;金香淑;;73例外周T細胞淋巴瘤患者臨床特征及預后分析[J];中南大學學報(醫(yī)學版);2008年02期

6 周立強;孫燕;譚文勇;李陶;王琦路;馮鳳儀;王金萬;儲大同;石遠凱;李曄雄;孫云田;呂寧;;非霍奇金淋巴瘤1125例臨床病理分析[J];癌癥進展;2006年05期

,

本文編號:1621691

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/zlx/1621691.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權申明:資料由用戶9ec60***提供,本站僅收錄摘要或目錄,作者需要刪除請E-mail郵箱bigeng88@qq.com
久久精品国产亚洲熟女| 一区二区三区在线不卡免费| 九九热最新视频免费观看| 99久只有精品免费视频播放| 日韩毛片视频免费观看| 亚洲午夜精品视频观看| 深夜福利亚洲高清性感| 黄片在线观看一区二区三区| 日本高清不卡在线一区| 久久精品一区二区少妇| 欧美激情一区二区亚洲专区| 99国产精品国产精品九九| 亚洲中文字幕乱码亚洲| 欧美日韩综合在线第一页| 国产偷拍盗摄一区二区| 东北老熟妇全程露脸被内射| 国产一区二区三区精品免费| 最新69国产精品视频| 熟女少妇一区二区三区蜜桃| 日韩精品亚洲精品国产精品| 精品欧美日韩一区二区三区| 色哟哟哟在线观看视频| 丰满的人妻一区二区三区| 亚洲一区精品二人人爽久久| 麻豆果冻传媒一二三区| 国产原创激情一区二区三区| 在线九月婷婷丁香伊人| 中文字幕精品一区二区年下载| 国产又大又黄又粗又免费| 尹人大香蕉中文在线播放| 亚洲中文字幕在线乱码av| 国产一区二区不卡在线播放| 日本高清一道一二三区四五区| 久久精品福利在线观看| 九九热九九热九九热九九热| 国产亚洲精品久久99| 激情偷拍一区二区三区视频| 国产精品一区二区视频大全| 人妻少妇系列中文字幕| 黄色日韩欧美在线观看| 欧美一区二区三区视频区|