本文選題：涎腺腫瘤　切入點(diǎn)：硒結(jié)合蛋白1　出處：《安徽醫(yī)科大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的硒結(jié)合蛋白1(Selenium binding protein 1,SBP1)位于染色體lq21-22上,是一種特殊的含硒蛋白質(zhì),主要是通過促進(jìn)腫瘤細(xì)胞的凋亡,以抑制腫瘤的發(fā)生。本課題檢測(cè)SBP1在涎腺腫瘤(salivary gland tumor)中的表達(dá)水平,并明確其與口腔腫瘤細(xì)胞—SCC3細(xì)胞的發(fā)生發(fā)展、侵襲以及轉(zhuǎn)移的關(guān)系,探討SBP1作為口腔腫瘤的腫瘤生物標(biāo)志物,評(píng)估臨床預(yù)后的潛能。方法收集手術(shù)切除的經(jīng)病理證實(shí)的15例良性涎腺腫瘤及瘤旁組織(多形性腺瘤),分別提取組織RNA,采用實(shí)時(shí)熒光定量PCR檢測(cè)組織中SBP1的表達(dá)水平。分別構(gòu)建慢病毒載體介導(dǎo)的SBP1的沉默和過表達(dá)的SCC3細(xì)胞,實(shí)時(shí)定量PCR檢測(cè)慢病毒轉(zhuǎn)染效率。應(yīng)用細(xì)胞劃痕實(shí)驗(yàn)檢測(cè)SBP1沉默和過表達(dá)后,腫瘤細(xì)胞遷移能力的改變;采用MTT檢測(cè)SBP1沉默和過表達(dá)后,腫瘤細(xì)胞增殖能力的變化。結(jié)果1.慢病毒能夠有效改變目的基因表達(dá),具有很高的轉(zhuǎn)染效率。2.實(shí)時(shí)熒光定量PCR檢測(cè)SBP1在涎腺腫瘤組織中的表達(dá)明顯低于正常涎腺組織(P0.05)。3.劃痕實(shí)驗(yàn)檢測(cè)沉默SBP1能使SCC3細(xì)胞的遷移能力加快,SBP1過表達(dá)時(shí)SCC3細(xì)胞的遷移能力減慢。4.MTT實(shí)驗(yàn)顯示沉默SBP1促進(jìn)SCC3細(xì)胞的增殖,過表達(dá)SBP1抑制SCC3細(xì)胞的增殖。結(jié)論1.SBP1在涎腺腫瘤組織和瘤旁組織的表達(dá)存在差異性,結(jié)果具有統(tǒng)計(jì)學(xué)意義。2.SBP1具有抑制SCC3細(xì)胞的增殖與遷移能力。3.SBP1可能是口腔腫瘤的早期診斷指標(biāo)之一。
[Abstract]:Objective selenium binding protein 1 SBP1) is a special protein containing selenium, which is located on chromosome lq21-22, mainly by promoting apoptosis of tumor cells to inhibit the occurrence of tumor. In this study, the expression level of SBP1 in salivary gland tumor was detected. The relationship between SBP1 and the occurrence, development, invasion and metastasis of oral tumor cell line-SCC3 was also discussed, and SBP1 was used as a biomarker of oral tumor. Methods Fifteen patients with pathologically proved benign salivary gland tumors and adjacent tissues (pleomorphic adenoma) were collected and their SBP1 expression was detected by real-time fluorescence quantitative PCR. Lentivirus vector mediated SBP1 silencing and overexpression of SCC3 cells were constructed. Real-time quantitative PCR was used to detect the efficiency of lentivirus transfection. Cell scratch assay was used to detect the change of tumor cell migration ability after SBP1 silencing and overexpression, and MTT was used to detect SBP1 silencing and overexpression. Results 1. Lentivirus can effectively change the expression of target gene. 2. The expression of SBP1 in salivary gland tumor tissue was significantly lower than that in normal salivary gland tissue (P 0.05N. 3). SBP1 silencing by scratch test could accelerate the migration ability of SCC3 cells. 4. MTT assay showed that silencing SBP1 could promote the proliferation of SCC3 cells. Overexpression of SBP1 inhibits the proliferation of SCC3 cells. 1. There are differences in the expression of SBP1 in salivary gland tumor tissues and adjacent tissues. Results SBP1 has the ability to inhibit the proliferation and migration of SCC3 cells. 3. SBP1 may be one of the early diagnostic markers of oral tumor.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R739.85

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本文編號(hào)：1618956