本文選題：去甲斑蝥酸鈉　切入點：順鉑　出處：《吉林大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:本研究通過研究不同濃度的傳統(tǒng)中藥姜黃素、去甲斑蝥酸鈉和化療藥物順鉑在不同時間,單藥以及聯(lián)合用藥對人乳腺癌MCF-7細胞增殖抑制的作用以及誘導凋亡和細胞周期的影響,初步探討去甲斑蝥酸鈉與順鉑聯(lián)用抑制人乳腺癌MCF-7細胞株可能的作用機制,同時對比單獨用藥與聯(lián)合用藥細胞增殖抑制作用的情況,從而為乳腺癌治療策略提供有效的基礎實驗數據做參考。方法:1、人乳腺癌細胞MCF-7細胞體外培養(yǎng)至對數生長期。2、通過MTT法檢測不同濃度的姜黃素、去甲斑蝥酸鈉和順鉑以及兩藥聯(lián)合作用于MCF-7細胞24、48和72小時后細胞增殖的情況。3、使用流式細胞儀檢測去甲斑蝥酸鈉(30μg/ml、15μg/ml)、順鉑(1.25μg/ml、0.625μg/ml)單獨以及聯(lián)合作用于MCF-7細胞48小時后誘導凋亡的情況和細胞周期的影響。結果:1.姜黃素、去甲斑蝥酸鈉和順鉑對MCF-7細胞增殖的抑制情況去甲斑蝥酸鈉和順鉑對MCF-7細胞均有增殖抑制作用,并且抑制效果呈時間-濃度依賴性的表現(xiàn),姜黃素通過實驗的統(tǒng)計結果來看對MCF-7無明顯抑制作用。2.不同濃度去甲斑蝥酸鈉與順鉑聯(lián)用對人乳腺腺癌MCF-7細胞增殖的抑制作用聯(lián)合應用去甲斑蝥酸鈉(30μg/ml、15μg/ml)和順鉑(1.25μg/ml、0.625μg/ml),對人乳腺癌MCF-7細胞的抑制作用均高于單獨用藥組,隨著去甲斑蝥酸鈉和順鉑的濃度增加,抑制作用增強,同時,單純的增加去甲斑蝥酸鈉的濃度,對MCF-7的抑制作用也在增強,隨著作用時間的延長抑制作用增強。3.去甲斑蝥酸鈉、順鉑以及聯(lián)合用藥誘導細胞凋亡作用及細胞周期的影響將MCF-7用去甲斑蝥酸鈉(30μg/ml、15μg/ml)處理48h后,G0/G1期細胞比例明顯縮小,處于S期細胞比例增多,流式細胞檢測結果表示人乳腺癌MCF-7細胞被去甲斑蝥酸鈉阻滯在S期(p0.05),影響細胞無法向G2/M期轉化,阻滯乳腺癌MCF-7細胞的脫氧核糖核酸合成,抑制腫瘤細胞的增殖進行。順鉑(1.25μg/ml、0.625μg/ml)作用于MCF-7細胞48h之后與對照組相比較,由細胞分布情況可見,G0/G1期細胞比例顯著縮小,S期細胞比例明顯增加,結果證明順鉑能夠將人乳腺癌細胞增殖抑制在S期(p0.05),從而使細胞不能向G2/M期轉化,進而實現(xiàn)對腫瘤細胞的增殖抑制作用。去甲斑蝥酸鈉、順鉑聯(lián)合作用于人乳腺癌MCF-7細胞的細胞凋亡率較單獨用藥組顯著增加(p0.01)結論:1.去甲斑蝥酸鈉、順鉑都可以抑制MCF-7細胞的增殖,在一定范圍內,呈時間-濃度依賴性。2.同純藥組相比較,去甲斑蝥酸鈉、順鉑兩藥聯(lián)合后,對MCF-7細胞增殖抑制作用明顯加強。3.去甲斑蝥酸鈉、順鉑都可以使MCF-7細胞周期阻滯在S期,而聯(lián)合用藥組的細胞凋亡率顯著高于單藥組。
[Abstract]:Objective: to study the different concentrations of curcumin, sodium norcantharidate and cisplatin in different concentrations. The effects of monotherapy and combination on the proliferation inhibition, apoptosis induction and cell cycle of human breast cancer MCF-7 cell line were studied. The possible mechanism of sodium norcantharidate combined with cisplatin in inhibiting human breast cancer MCF-7 cell line was discussed. At the same time, the inhibition of cell proliferation was compared between single and combined drugs. So as to provide effective basic experimental data for breast cancer treatment strategy. Methods: 1. Human breast cancer MCF-7 cells were cultured to logarithmic growth phase in vitro. Curcumin at different concentrations was detected by MTT assay. Effects of sodium norcantharidate, cisplatin and two drugs on proliferation of MCF-7 cells after 24 hours and 72 hours. Flow cytometry was used to detect sodium norcantharidate 30 渭 g / ml 15 渭 g / ml, cisplatin 1.25 渭 g / ml / ml 0.625 渭 g / ml) alone and in combination with MCF-7 cells for 48 hours. Effects of apoptosis and cell cycle. Results: 1. Curcumin, curcumin, curcumin, curcumin, curcumin, curcumin, Inhibition of proliferation of MCF-7 cells by sodium norcantharidate and cisplatin. Sodium norcantharidate and cisplatin inhibited proliferation of MCF-7 cells in a time-concentration dependent manner. Curcumin had no obvious inhibitory effect on MCF-7 by statistical results. 2. The inhibitory effect of sodium norcantharidate and cisplatin on the proliferation of human breast adenocarcinoma MCF-7 cells combined with sodium norcantharidate 30 渭 g / ml 15 渭 g / ml and cisplatin 1.25 渭 g / ml 0.625 渭 g / ml. The inhibitory effects on human breast cancer MCF-7 cells were higher than those in the control group. With the increase of the concentration of norcantharidate and cisplatin, the inhibitory effect of sodium norcantharidate and cisplatin was enhanced. At the same time, the inhibitory effect of sodium norcantharidate and sodium norcantharidate on MCF-7 was also increased, and the inhibitory effect of sodium norcantharidate was enhanced with the prolongation of time. Apoptosis induced by cisplatin and combination of Cisplatin and its effect on Cell cycle the proportion of cells in G _ 0 / G _ 1 phase of MCF-7 treated with sodium norcantharidate 30 渭 g 路ml ~ (-1) 15 渭 g / ml for 48 h significantly decreased, and the proportion of cells in S phase increased. The results of flow cytometry showed that human breast cancer MCF-7 cells were blocked by sodium norcantharidate in S phase, which affected the cell transformation to G 2 / M phase, and blocked the synthesis of DNA in breast cancer MCF-7 cells. After treated with cisplatin 1.25 渭 g / ml, 0.625 渭 g / ml of cisplatin for 48 hours, compared with the control group, the proportion of cells in G _ 0 / G _ 1 phase decreased significantly and the proportion of cells in S phase increased significantly compared with the control group. The results showed that cisplatin could inhibit the proliferation of human breast cancer cells in S phase, thus the cells could not be transformed into G2 / M phase, and then the proliferation inhibition of tumor cells could be realized. The apoptotic rate of cisplatin combined with cisplatin on human breast cancer MCF-7 cells was significantly higher than that in the control group (P 0.01). Conclusion: 1. Sodium norcantharidate and cisplatin can inhibit the proliferation of MCF-7 cells. Compared with the pure drug group, the inhibitory effect of sodium norcantharidate and cisplatin on the proliferation of MCF-7 cells was significantly enhanced. 3. Sodium norcantharidate and cisplatin could block the cell cycle of MCF-7 in S phase. The apoptosis rate of the combined drug group was significantly higher than that of the single drug group.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.9

【參考文獻】

相關期刊論文 前7條

1 蔡勇;王季穎;;姜黃素聯(lián)合順鉑對非小細胞肺癌細胞A549放療增敏作用的初步研究[J];臨床腫瘤學雜志;2015年01期

2 賈丹;張苗苗;李楠;王家仁;許瓊明;劉艷麗;劉江云;楊世林;;艾迪注射液中刺五加提取物對斑蝥素的減毒增效作用[J];蘇州大學學報(醫(yī)學版);2012年03期

3 曾歡;宋興福;董自強;;蟾蜍制劑誘導腫瘤細胞凋亡的研究進展[J];現(xiàn)代腫瘤醫(yī)學;2012年04期

4 夏環(huán)玲;;復方斑蝥膠囊聯(lián)合放化療治療晚期食管癌的療效觀察[J];中國腫瘤臨床與康復;2012年02期

5 魏方超;杜娟;未寧寧;王巖;李尚偉;;斑蝥素及其衍生物的研究現(xiàn)狀與應用[J];現(xiàn)代生物醫(yī)學進展;2012年08期

6 王景毅;梁金龍;劉國津;;去甲斑蝥素對乳腺癌細胞血管內皮生長因子表達的影響[J];中華中醫(yī)藥學刊;2009年11期

7 范躍祖;陳春球;趙澤明;孫偉;;去甲斑蝥素對膽囊癌腫瘤血管生成的作用及機制研究[J];中華醫(yī)學雜志;2006年10期

相關博士學位論文 前1條

1 陳萍;姜黃素逆轉肺癌細胞對順鉑耐藥性的FA/BRCA途徑機制研究[D];江蘇大學;2014年

，

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