本文選題：結(jié)直腸腺癌　切入點(diǎn)：Notch3　出處：《遵義醫(yī)學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:檢測Notch3、DLL1、CD133在正常人結(jié)直腸組織、結(jié)直腸腺瘤、結(jié)直腸腺癌組織中的表達(dá)情況,探討以上指標(biāo)在結(jié)直腸癌發(fā)生、發(fā)展過程中的作用及其與臨床病理特征間的關(guān)系。方法:采用免疫組織化學(xué)的方法檢測12例正常結(jié)直腸黏膜、30例結(jié)直腸腺瘤、50例結(jié)直腸腺癌組織中Notch3、DLL1、CD133的表達(dá)情況,分析上述指標(biāo)的表達(dá)與臨床病理特征間的關(guān)系,并運(yùn)用Spearman相關(guān)分析法對3組指標(biāo)的表達(dá)情況進(jìn)行相關(guān)性分析。結(jié)果:在正常結(jié)直腸黏膜、結(jié)直腸腺瘤、結(jié)直腸腺癌組織中Notch3的陽性表達(dá)率分別為8.3%(1/12)、26.7%(8/30)、64%(32/50);DLL1的陽性表達(dá)率分別為16.7%(2/12)、33.3%(10/30)、68.0%(34/50);CD133的陽性陽性表達(dá)率分別為8.3%(1/12)、36.7%(11/30)、54.0%(27/50)。上述指標(biāo)在腺癌組的陽性表達(dá)率均明顯高于腺瘤組及正常組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);腺瘤組與正常組相比,差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。Notch3、DLL1、CD133在結(jié)直腸腺癌組織中的表達(dá)與性別、年齡、腫瘤部位、腫瘤大小及分化程度無關(guān)(P0.05),而均與淋巴結(jié)轉(zhuǎn)移有關(guān),伴有淋巴結(jié)轉(zhuǎn)移組陽性表達(dá)率明顯高于無淋巴結(jié)轉(zhuǎn)移組(P0.05),且Notch3和DLL1的表達(dá)與Dukes分期有關(guān),Dukes分期分期越高陽性表達(dá)率越高(P0.05),此外DLL1的表達(dá)與腫瘤浸潤深度也有關(guān),腫瘤浸潤深度越深陽性表達(dá)率越高(P0.05)。Notch3蛋白和CD133蛋白表達(dá)呈正相關(guān)關(guān)系(r=0.478,P0.01)。其余指標(biāo)間均無明顯相關(guān)性。結(jié)論:1.Notch3、DLL1、CD133在正常結(jié)直腸組織、結(jié)直腸腺瘤、結(jié)直腸腺癌組織中的表達(dá)呈逐漸遞增趨勢,他們可能參與了結(jié)直腸癌的發(fā)生、發(fā)展過程。2.Notch3蛋白表達(dá)與淋巴結(jié)轉(zhuǎn)移和Dukes分期有關(guān),DLL1蛋白表達(dá)與腫瘤浸潤深度和淋巴結(jié)轉(zhuǎn)移有關(guān),CD133蛋白表達(dá)與淋巴結(jié)轉(zhuǎn)移有關(guān);提示以上3個指標(biāo)可有望成為新的腫瘤標(biāo)志物推測結(jié)直腸癌患者的預(yù)后。3.Notch3蛋白與CD133蛋白的表達(dá)存在明顯正相關(guān)關(guān)系。
[Abstract]:Objective: to detect the expression of Notch3DLL1CD133 in normal human colorectal tissues, colorectal adenomas and colorectal adenomas, and to investigate the occurrence of these markers in colorectal cancer. Methods: immunohistochemical method was used to detect the expression of Notch3- DL1 CD133 in 30 cases of colorectal adenoma and 50 cases of colorectal adenocarcinoma. To analyze the relationship between the expression of the above indexes and the clinicopathological features, and to analyze the correlation between the expression of the above indexes and the clinicopathological characteristics of the three groups by Spearman correlation analysis. Results: in normal colorectal mucosa, colorectal adenoma, The positive expression rates of Notch3 in colorectal adenocarcinoma tissues were 8.31 / 12 / 26.7 and 8 / 30 / 32 / 30, respectively. The positive expression rates of DLL1 in colorectal adenocarcinoma tissues were 16.775 / 12 / 33. 3 / 10 / 30 / 68.0 / 34 / 34 / 50 CD133, respectively. The positive expression rates of the above indexes in adenoma group were significantly higher than those in adenoma group and normal group, and the positive expression rate was 8.31 / 12 / 36.7T / 11 / 3054.30 / 27500.The positive expression rate of these indexes in adenocarcinoma group was significantly higher than that in adenoma group and normal group, and the positive expression rate of DLL1 in adenocarcinoma group was significantly higher than that in adenoma group and normal group, and the positive expression rate of DLL1 in adenocarcinoma group was significantly higher than that in adenoma group and normal group. There was no significant difference between adenoma group and normal group. The expression of CD133 in colorectal adenocarcinoma was not related to sex, age, tumor location, tumor size and differentiation degree, but was related to lymph node metastasis. The positive expression rate of Notch3 and DLL1 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis, and the expression of Notch3 and DLL1 was related to the Dukes staging. The higher the positive rate of Dukes staging was, the higher the positive expression rate of DLL1 was. In addition, the expression of DLL1 was also related to the depth of tumor invasion. The deeper the depth of tumor invasion, the higher the positive expression rate of P0.05N. Notch3 protein and CD133 protein expression. There was no significant correlation between the other markers. Conclusion: 1. Notch3 DLL1 CD133 in normal colorectal tissue and colorectal adenoma. The expression of colorectal adenocarcinoma is increasing gradually, and they may be involved in the development of colorectal cancer. 2. The expression of Notch3 protein was related to lymph node metastasis and Dukes stage. The expression of DLL1 protein was related to the depth of tumor invasion and lymph node metastasis, and the expression of CD133 protein was related to lymph node metastasis. The results suggest that the above three markers may be a new tumor marker for predicting a positive correlation between the expression of CD133 protein and the prognosis of colorectal cancer patients. 3.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.34

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