發(fā)布時間：2018-03-11 15:14

  本文選題：膀胱癌　切入點：wnt信號　出處：《內(nèi)蒙古大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:檢測WIF1在膀胱癌組織與血清中的表達水平,并分析其與MMP-9的相關(guān)性,探討其在膀胱癌的發(fā)生發(fā)展中的調(diào)節(jié)機制。方法:1、收集病例,分為實驗組與對照組,實驗組為膀胱癌組織,對照組為膀胱癌旁正常組織;實驗組被分為高分化組與低分化組及浸潤組與非浸潤組。2、Real-time PCR檢測WIF1在膀胱癌組織與癌旁正常組織、高分化組與低分化組、浸潤組與非浸潤組的基因表達。4、ELISA檢測WIF1在膀胱癌高分化組與低分化組、浸潤組與非浸潤組血清蛋白表達。5、分別檢測WIF1與MMP-9在膀胱癌浸潤組的血清蛋白含量,分析二者的相關(guān)性。結(jié)果:1、在實驗組與癌旁對照組檢測WIF1基因表達水平顯示,實驗組的基因表達量(0.23 ±0.131)明顯低于癌旁對照組(0.54±0.170),差異具有統(tǒng)計學意義(t=-0.710、P0.001);在低分化組WIF1基因表達量(0.16 ± 0.082)明顯低于高分化組(0.26±0.088),差異具有統(tǒng)計學意義(t=-3.135、P0.01);在浸潤組WIF1基因表達量(0.14±0.087)明顯低于非浸潤組(0.28±0.135),差異具有統(tǒng)計學意義(t=-3.204、P0.01)。2、WIF1在血清中蛋白表達檢測顯示,在低分化組的表達水平(21.85±5.833)明顯低于高分化組(30.35±8.040),差異具有統(tǒng)計學意義(t=-3.203、P0.01);WIF1蛋白在浸潤組的表達量(21.56±5.141)明顯低于非浸潤組(31.97土9.064),差異具有統(tǒng)計學意義(t=-3.599、P0.01);在浸潤組,WIF1蛋白的表達與MMP-9蛋白表達具有相關(guān)性,呈負相關(guān)(r=-0.643、p0.05)。結(jié)論:1.WIF1與膀胱癌的發(fā)生、侵襲浸潤密切相關(guān),其在膀胱癌的發(fā)生、侵襲浸潤中可能發(fā)揮重要調(diào)節(jié)功能,可能是通過調(diào)節(jié)經(jīng)典wnt信號通路發(fā)揮作用。2.WIF1在血清中的異常表達可能為臨床的早期發(fā)現(xiàn)提供有效證據(jù),可能為膀胱癌的病理分級、侵襲浸潤能力及術(shù)后預(yù)后的判斷提供簡單有效的檢測方法。3.在膀胱癌的侵襲浸潤中,WIF1可能通過促進MMP-9的表達進而發(fā)揮其侵襲浸潤功能,其可能通過激活經(jīng)典wnt信號通路發(fā)揮作用,或二者之間存在獨立信號機制。
[Abstract]:Objective: to detect the expression of WIF1 in bladder cancer tissues and serum, and to analyze its correlation with MMP-9, and to explore its regulatory mechanism in the occurrence and development of bladder cancer. Methods: one case was collected and divided into experimental group and control group. The experimental group was divided into high differentiation group and low differentiation group, and infiltration group and non-invasive group. Real-time PCR was used to detect WIF1 in bladder cancer tissue and adjacent normal tissue, high differentiation group and low differentiation group. The expression of WIF1 was detected by Elisa in high differentiation and low differentiation groups, infiltration group and non invasive group. The serum protein levels of WIF1 and MMP-9 in bladder cancer infiltrating group were detected respectively. Results: the expression level of WIF1 gene was detected in the experimental group and the adjacent control group. The expression of WIF1 gene in the experimental group (0.23 鹵0.131) was significantly lower than that in the control group (0.54 鹵0.170), the difference was statistically significant, and the expression of WIF1 gene in the poorly differentiated group (0.16 鹵0.082) was significantly lower than that in the well-differentiated group (0.26 鹵0.088). The expression level of WIF1 in serum was significantly lower than that in non-invasive group (0.14 鹵0.087), and the difference was statistically significant. The expression level of WIF1 protein in the poorly differentiated group (21.85 鹵5.833) was significantly lower than that in the well-differentiated group (30.35 鹵8.040), the difference was statistically significant (P < 21.56 鹵5.141), and the expression of WIF1 protein in the infiltrating group was significantly lower than that in the non-invasive group (31.97 鹵9.064), the difference was statistically significant (P 0.01), and the expression of WIF1 protein in the infiltrating group was significantly lower than that in the non-invasive group. The expression of MMP-9 was correlated with the expression of MMP-9 protein. Conclusion: 1. WIF1 is closely related to the occurrence and invasion of bladder cancer, which may play an important regulatory role in the occurrence and invasion of bladder cancer. The abnormal expression of WIF1 in serum may provide effective evidence for early clinical discovery and may be the pathological grade of bladder cancer. Evaluation of invasive invasion ability and postoperative prognosis provides a simple and effective method for detecting invasion and invasion of bladder cancer. WIF1 may play an invasive and invasive function by promoting the expression of MMP-9 in bladder cancer invasion and invasion. It may play a role by activating the classical wnt signaling pathway, or there is an independent signal mechanism between the two.
【學位授予單位】：內(nèi)蒙古大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.14

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相關(guān)博士學位論文 前1條

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