本文選題：喉鱗狀細(xì)胞癌　切入點(diǎn)：ALDOA　出處：《河北醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:研究喉鱗狀細(xì)胞癌腫瘤組織中醛縮酶(aldolase A,ALDOA)、葡萄糖6磷酸脫氫酶(Glucose-6-phosphate,G6PD)、磷酸果糖激酶1(Phosphofructokinase-1,PFK1)的表達(dá)情況、以及三者間關(guān)系及與臨床病理參數(shù)的關(guān)系,進(jìn)一步探討腫瘤組織細(xì)胞中糖酵解和磷酸戊糖途徑在代謝等方面的潛在關(guān)系,并揭示這三種蛋白與喉鱗狀細(xì)胞癌的發(fā)生、增殖、侵襲、轉(zhuǎn)移等過程中的作用。方法:選取在河北醫(yī)科大學(xué)第二醫(yī)院2015年6月-2016年12月間40例行手術(shù)治療的喉癌病人的標(biāo)本,并經(jīng)病理鑒定,確定這40例喉腫瘤標(biāo)本均為鱗狀細(xì)胞癌,此外,選取40例癌旁組織(距腫瘤邊沿距離0.5cm,經(jīng)病理證實(shí)未見腫瘤細(xì)胞浸潤)作為對(duì)照,喉鱗狀細(xì)胞癌組和對(duì)照組患者手術(shù)前均未接受過放化療及其他相關(guān)治療。本實(shí)驗(yàn)采取免疫組織化學(xué)染色法中免疫酶細(xì)胞化學(xué)技術(shù)檢測(cè)喉鱗狀細(xì)胞癌腫瘤組織及癌旁組織中ALDOA、PFK1和G6PD蛋白的表達(dá)情況,實(shí)驗(yàn)數(shù)據(jù)通過SPSS13.0軟件分析,采用χ2檢驗(yàn)方法分析喉癌及癌旁組織中這三種蛋白表達(dá)的陽性率,并分析這三者水平之間的差異,還分別分析這三種蛋白與喉癌患者的臨床上的病理參數(shù)的關(guān)系,采用Spearman等級(jí)相關(guān)方法檢測(cè)ALDOA、G6PD和PFK1蛋白間的相互關(guān)系,統(tǒng)計(jì)學(xué)以雙側(cè)P0.05有顯著意義。結(jié)果:1在喉癌和癌旁組織中ALDOA、G6PD、PFK1蛋白表達(dá)的陽性率分別為65.0%和15.0%、55.0%和20.0%、52.5%和30.0%,各兩組間相比,差異均有顯著的統(tǒng)計(jì)學(xué)意義(P0.05);在喉癌組中ALDOA、G6PD、PFK1蛋白在臨床分期為I~II期及III~IV期表達(dá)的陽性率分別為44.4%和81.8%、33.3%和72.7%、33.3%和68.2%,各兩組間差異比較均有顯著的統(tǒng)計(jì)學(xué)意義(P0.05);在病理為高分化組及中~低分化組的喉癌患者中ALDOA、G6PD、PFK1蛋白表達(dá)的陽性率分別為47.6%和84.2%、33.3%和78.9%、28.6%和78.9%,各兩組間差異對(duì)比均有顯著的統(tǒng)計(jì)學(xué)意義;ALDOA、G6PD、PFK1蛋白分別在有和無淋巴結(jié)轉(zhuǎn)移組的喉癌患者中表達(dá)的陽性分別為78.6%和33.3%、67.9%和25.0%、64.3%和25.0%,各兩組間相比差異均有顯著統(tǒng)計(jì)學(xué)意義(P0.05);在年齡≥60歲組及60歲組的喉癌組中ALDOA、G6PD、PFK1蛋白表達(dá)的陽性率分別為65.2%和65.7%、47.8%和64.7%、47.8%和58.8%,各兩組間差異比較均無顯著統(tǒng)計(jì)學(xué)意義(P0.05);ALDOA、G6PD、PFK1蛋白在吸煙≥20支/天組及20支/天組的喉癌病人中表達(dá)的陽性率分別為61.5%和66.7%、38.5%和63.0%、38.5%和59.3%,各兩組間結(jié)果比較均無顯著統(tǒng)計(jì)學(xué)意義(P0.05)。2在喉癌的病人中ALDOA蛋白與G6PD蛋白的水平呈正相關(guān)(相關(guān)系數(shù)r=0.601,P0.05);而且ALDOA蛋白與PFK1蛋白在喉癌中的水平也呈正相關(guān)(相關(guān)系數(shù)r=0.457,P0.05);還有PFK1蛋白與G6PD蛋白的水平也呈正相關(guān)表現(xiàn)(相關(guān)系數(shù)r=0.750,P0.05)。結(jié)論:1在喉鱗狀細(xì)胞癌組中ALDOA、G6PD及PFK1蛋白水平均明顯高于癌旁組,而且這三種蛋白的水平與淋巴結(jié)轉(zhuǎn)移與否、病理分化程度有關(guān),另外三者的水平均還與喉癌臨床分期相關(guān),但是這三種蛋白的水平都與病人的年齡以及吸煙的數(shù)量無關(guān)。由此可見這三種蛋白在喉鱗狀細(xì)胞癌的形成、生長、細(xì)胞的增殖及腫瘤的轉(zhuǎn)移中起著一定的作用。2喉癌組中PFK1蛋白、G6PD蛋白水平均與ALDOA蛋白水平相關(guān),ALDOA可能是聯(lián)系喉癌中糖酵解和磷酸戊糖途徑的一個(gè)重要因子,這三種蛋白在腫瘤的形成、生長和轉(zhuǎn)移浸潤等過程中可能相互促進(jìn),緊密聯(lián)系,有協(xié)同作用。
[Abstract]:Objective: To study the tumor aldolase in laryngeal squamous cell carcinoma (aldolase, A, ALDOA), glucose 6 phosphate dehydrogenase (Glucose-6-phosphate, G6PD), phosphofructokinase 1 (Phosphofructokinase-1, PFK1) expression, and the relationship between the three and their relationship with clinical pathological parameters, to further explore the potential relationship between tumor cell glycolysis and the pentose phosphate pathway in the metabolism and other aspects, and reveals the three proteins and laryngeal squamous cell carcinoma, proliferation, invasion and metastasis process. Methods: a total of Second Hospital of Hebei Medical University in June 2015 -2016 year in December 40 cases of surgical treatment of patients with laryngeal cancer specimens by pathology and identification, to determine which 40 cases of laryngeal tumor specimens were squamous cell carcinoma, in addition, a total of 40 cases of paracancerous tissues (from tumor edge distance 0.5cm, confirmed by pathology and no tumor cell invasion) as control, laryngeal squamous Cell carcinoma group and control group patients before surgery had not received chemotherapy and other related treatment. This experiment adopt immunohistochemical staining ALDOA immunocytochemistry technique in detection of laryngeal squamous cell carcinoma tissues and adjacent tissues, the expression of PFK1 and G6PD proteins, the analysis of experimental data by using SPSS13.0 software. 2 test method for the analysis of the positive expression rate of the three proteins in laryngeal carcinoma and adjacent tissues, and analyze the difference between the three levels of relationship were also pathological analysis of the three proteins and clinical parameters of patients with laryngeal carcinoma on, using Spearman rank correlation method to detect ALDOA, G6PD and PFK1 protein interaction the statistics on bilateral P0.05 was significant. Results: ALDOA 1 in laryngeal carcinoma and adjacent tissues of G6PD, the positive rate of PFK1 protein expression were 65% and 15%, 55% and 20%, 52.5% and 30%, two in each group Compared with statistical significant differences (P0.05); ALDOA, G6PD in laryngeal carcinoma group, PFK1 protein in clinical stage positive expression rate of I~II and III~IV were 44.4% and 81.8%, 33.3% and 72.7%, 33.3% and 68.2%, the difference between the two groups were significant (P0.05) in high differentiation group; pathology and in low differentiation group of laryngeal cancer patients in ALDOA, G6PD, the positive rate of PFK1 protein expression were 47.6% and 84.2%, 33.3% and 78.9%, 28.6% and 78.9%, the difference between the two groups comparison was statistically significant; ALDOA, G6PD, PFK1 protein respectively with and without the expression of lymph node metastasis in patients with laryngeal cancer were respectively 78.6% and 33.3%, 67.9% and 25%, 64.3% and 25%, there was significant difference compared between the two groups (P0.05); ALDOA in aged 60 and 60 years of age group G6PD laryngeal carcinoma group, the positive rate of PFK1 protein expression Were 65.2% and 65.7%, 47.8% and 64.7%, 47.8% and 58.8%, the difference between the two groups were not statistically significant (P0.05); ALDOA, G6PD, PFK1 protein in smoking more than 20 cigarettes / day group and 20 day group / positive expression rate of laryngeal cancer patients were 61.5% and 66.7%, 38.5% and 63%, 38.5% and 59.3%, the two groups showed no statistically significant results (P0.05) levels were positively correlated with ALDOA in laryngeal carcinoma.2 protein and G6PD protein (correlation coefficient r=0.601, P0.05); and the level of ALDOA protein and PFK1 protein in laryngeal carcinoma and Cheng Zhengxiang (correlation coefficient r=0.457, P0.05); and PFK1 protein and G6PD protein expression level was positively correlated (correlation coefficient r=0.750, P0.05). Conclusion: 1 in laryngeal squamous cell carcinoma group ALDOA, G6PD and PFK1 protein levels were significantly higher than those in adjacent groups, and the water level of the three proteins with lymph node metastasis and pathological differentiation. To certain extent, the other three levels were also related and clinical stage, but these three kinds of protein levels and patient's age and smoking. Thus independent of the number of formation of these three proteins in laryngeal squamous cell carcinoma growth, plays a role of PFK1 protein in laryngeal carcinoma.2 group the proliferation and metastasis of tumor cells in the G6PD protein levels were correlated with ALDOA protein levels, ALDOA may be an important factor in the relationship between laryngeal glycolysis and the pentose phosphate pathway, the formation of these three proteins in tumor growth and metastasis, infiltration can promote each other, closely, have synergistic effect.

【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R739.65

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相關(guān)期刊論文 前1條

1 遲鑫明;胡軍;張麗媛;趙皓琦;郝立宏;;應(yīng)用組織芯片技術(shù)檢測(cè)果糖二磷酸醛縮酶在肝癌與胰腺癌組織中的表達(dá)[J];中國組織化學(xué)與細(xì)胞化學(xué)雜志;2014年06期

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本文編號(hào)：1575847