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  本文選題：胃癌　切入點：長鏈非編碼RNA(Long　出處：《寧夏醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:研究上皮-間質(zhì)轉(zhuǎn)化(Epithelial-mesenchymal transition,EMT)信號通路相關(guān)長鏈非編碼RNA(Long non-coding RNA,LncRNA)在胃癌組織及癌旁胃粘膜組織中的差異表達,探索代表性EMT相關(guān)lncRNA在胃癌臨床診斷中的潛在價值。方法:收集胃癌組織及癌旁胃粘膜組織,應用LncPath Human EMT Pathway LncRNA Microarray檢測胃癌組織和癌旁胃粘膜組織EMT相關(guān)lncRNA和相關(guān)靶基因的表達水平,篩選出表達差異明顯的lncRNA,并繪制與相關(guān)靶基因相互作用圖。實時定量逆轉(zhuǎn)錄-聚合酶鏈式反應(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)檢測胃癌組織、癌旁胃粘膜組織、胃癌細胞株BGC-823及正常胃粘膜細胞GES-1 lncRNA metallothionein 1J,pseudogene(MT1JP)和mRNA-metallothionein 1M(MT1M)的表達水平。結(jié)合臨床資料分析,MT1JP和MT1M表達水平與患者臨床病理因素之間的相關(guān)性,探索其在臨床診斷中的潛在價值。結(jié)果:胃癌組織與癌旁胃粘膜組織之間表達差異超過2倍lncRNA有26條,有16條明顯上調(diào),10條明顯下調(diào);qRT-PCR檢測結(jié)果LncRNA-MT1JP和靶基因mRNAMT1M的表達水平在胃癌組織中降低,MT1JP表達水平與腫瘤大小、分期、淋巴轉(zhuǎn)移、遠處轉(zhuǎn)移、TNM分期相關(guān)(P0.05)。MT1JP在胃癌前病變、早期胃癌中及進展期胃癌中表達依次降低。結(jié)論:胃癌中大量EMT相關(guān)lncRNA異常表達,可能通過EMT相關(guān)信號通路在胃癌發(fā)展中發(fā)揮調(diào)節(jié)作用。MT1JP和MT1M在胃癌組織中低表達,與腫瘤的分期及浸潤程度具有相關(guān)性。MT1JP在癌前病變及早期胃癌中低表達,可能是潛在的胃癌分子診斷和治療的靶標。
[Abstract]:Objective: to investigate the differential expression of Epithelial-mesenchymal transition (EMT) signaling pathway associated with long chain noncoding RNA(Long non-coding RNAs in gastric carcinoma and adjacent gastric mucosa. To explore the potential value of representative EMT associated lncRNA in the clinical diagnosis of gastric cancer methods: gastric cancer tissues and adjacent gastric mucosa tissues were collected. LncPath Human EMT Pathway LncRNA Microarray was used to detect the expression of EMT related lncRNA and related target genes in gastric carcinoma and adjacent gastric mucosa. The differentially expressed LNC RNAs were screened, and the interaction diagrams with related target genes were plotted. Real-time quantitative reverse transcription-polymerase chain reactionsqRT-PCRs were used to detect gastric cancer tissues and adjacent gastric mucosa. The expression levels of GES-1 lncRNA metallothionein 1J BGC-823 and mRNA-metallothionein 1Mt1MP) in gastric cancer cell line BGC-823 and normal gastric mucosal cells were analyzed. The correlation between the expression levels of MT1M and clinicopathological factors was analyzed in combination with clinical data. Results: the difference of expression between gastric cancer tissue and adjacent gastric mucosa was more than 2 times lncRNA. The expression level of LncRNA-MT1JP and target gene mRNAMT1M in gastric cancer tissues decreased significantly. The expression level of MT1JP and tumor size, staging, lymphatic metastasis and distant metastasis were correlated with P0.05. MT1JP in gastric precancerous lesions. Conclusion: a large number of abnormal expression of EMT related lncRNA may play a regulatory role in the development of gastric cancer through EMT related signaling pathway. MT1 JP and MT1M may play a low expression in gastric cancer tissues. The low expression of MT1JP in precancerous lesions and early gastric cancer may be a potential target for molecular diagnosis and treatment of gastric cancer.
【學位授予單位】：寧夏醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R735.2

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本文編號：1570222