本文關(guān)鍵詞： 胃癌 microcarrier MKN細(xì)胞 動(dòng)物模型　出處：《中國腫瘤臨床》2017年05期 　論文類型：期刊論文

【摘要】：目的:基于新型3D微載體(microcarrier 6)復(fù)合人胃癌MKN45細(xì)胞接種于具有正常免疫功能的小鼠,以期建立新型人胃癌正常小鼠轉(zhuǎn)移瘤模型。方法:將60只雄性C57BL/6小鼠按是否將MKN45細(xì)胞接種于支架材料隨機(jī)分為2D組、對(duì)照組和3D組;體外構(gòu)建三維腫瘤細(xì)胞培養(yǎng)模型,采用皮下接種法建立小鼠移植瘤模型,觀察小鼠成瘤時(shí)間、成瘤率、病理學(xué)表現(xiàn)等。結(jié)果:2D組和對(duì)照組均未成瘤,3D組成瘤率達(dá)80%,成瘤時(shí)間早,移植瘤HE染色和免疫組織化學(xué)均符合人胃癌特點(diǎn)。結(jié)論:本實(shí)驗(yàn)成功基于microcarrier 6復(fù)合人胃癌MKN45細(xì)胞在正常免疫小鼠中建立人胃癌小鼠移植瘤模型,此模型可以更好地研究和進(jìn)一步闡明胃癌在免疫功能正常機(jī)體中發(fā)生、發(fā)展機(jī)制,同時(shí)也為抗癌藥物的研發(fā)提供了更有價(jià)值的動(dòng)物模型。
[Abstract]:Objective: to inoculate human gastric cancer MKN45 cells based on 3D microcarrier 6 in mice with normal immune function. Methods: 60 male C57BL / 6 mice were randomly divided into 2D group, control group and 3D group according to whether the MKN45 cells were inoculated into scaffold materials. The model of mouse transplanted tumor was established by subcutaneous inoculation. The tumorigenesis time, tumorigenesis rate and pathological manifestation of mice were observed. Both HE staining and immunohistochemistry were consistent with the characteristics of human gastric cancer. Conclusion: the transplanted tumor model of human gastric cancer mice was successfully established in normal immunized mice based on microcarrier 6 combined with human gastric cancer MKN45 cells. This model can better study and further elucidate the mechanism of gastric cancer in normal immune system and provide a more valuable animal model for the development of anticancer drugs.
【作者單位】： 山東大學(xué)醫(yī)學(xué)院;濟(jì)寧醫(yī)學(xué)院附屬醫(yī)院;大理大學(xué) 云南省昆蟲生物醫(yī)藥研發(fā)重點(diǎn)實(shí)驗(yàn)室 藥用特種昆蟲開發(fā)國家地方聯(lián)合工程研究中心;濟(jì)南大學(xué)醫(yī)學(xué)與生命科學(xué)學(xué)院;山東省寄生蟲病防治研究所;University
【基金】：國家自然科學(xué)基金項(xiàng)目(編號(hào):81170395,81570556,81260676)資助~~
【分類號(hào)】：R735.2;R-332
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本文編號(hào)：1547852