本文關(guān)鍵詞： Klotho Survivin 胃腸道神經(jīng)內(nèi)分泌癌　出處：《南華大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:檢測Survivin和Klotho在胃腸道神經(jīng)內(nèi)分泌癌患者癌組織及癌旁組織中表達(dá),探討Survivin和Klotho與胃腸道神經(jīng)內(nèi)分泌癌患者的預(yù)后的相關(guān)性。方法:對51例胃腸道神經(jīng)內(nèi)分泌癌患者的病例資料進(jìn)行回顧性研究,運(yùn)用免疫組化和Western blot法來檢測GNT患者癌組織及癌旁組織Survivin和Klotho的表達(dá);分析和研究兩種蛋白質(zhì)的表達(dá)與胃腸道神經(jīng)內(nèi)分泌癌患者預(yù)后的相關(guān)性。結(jié)果:1.在51例胃腸道神經(jīng)內(nèi)分泌癌患者中有35例(68.63%)患者癌組織Survivin陽性,癌旁組織中未發(fā)現(xiàn)有Survivin的表達(dá)。運(yùn)用免疫組化、Western Blot檢測Survivi在GNT癌組織和癌旁組織中的表達(dá)情況。免疫組化結(jié)果顯示小細(xì)胞癌中Survivin的表達(dá)強(qiáng)度和數(shù)量(18/21)高于大細(xì)胞癌(14/30),癌旁組織中Survivin表達(dá)兩者無差異。臨床資料發(fā)現(xiàn)Survivin的表達(dá)與腫瘤分期和組織分化相關(guān),與年齡、性別等其他因素?zé)o關(guān)。分析顯示低分化及未分化的癌組織中Survivin的表達(dá)顯著高于高分化癌(P=0.0013),其中在臨床分期Ⅲ期(5/7)、Ⅳ期(12/12)中Survivin的陽性表達(dá)率為100%,顯著高于Ⅱ期(15/27)Ⅰ期(1/5)期癌組織中Survivin的表達(dá)(P=0.0023)。2.51例腫瘤標(biāo)本免疫組化檢測Klotho發(fā)現(xiàn)所有標(biāo)本癌旁組織Klotho的表達(dá)均呈強(qiáng)陽性,21例標(biāo)本的胃腸道神經(jīng)內(nèi)分泌癌組織Klotho呈現(xiàn)弱陽性反應(yīng);Western Blot檢測Klotho發(fā)現(xiàn)胃腸道神經(jīng)內(nèi)分泌癌旁組織中的Klotho的表達(dá)顯著高于癌組織。Klotho的表達(dá)與組織分化和腫瘤分期相關(guān),與年齡等其他因素?zé)o關(guān)。Ⅳ期的患者中沒有檢測到Klotho的表達(dá),高分化者(6/7)的癌組織中Klotho的表達(dá)顯著高于低分化、未分化者(0/6,0/4,P=0.0035),在Ⅰ期患者的Klotho的陽性表達(dá)率為(5/5)顯著高于Ⅱ(13/27)和Ⅲ(2/7)期患者(P=0.0067)。結(jié)論:1.胃腸道神經(jīng)內(nèi)分泌癌患者中,Survivin陽性表達(dá)小細(xì)胞癌顯著高于大細(xì)胞癌,大細(xì)胞癌中顯著高于癌旁組織;2.胃腸道神經(jīng)內(nèi)分泌癌患者中,Klotho陽性表達(dá)小細(xì)胞癌顯著低于大細(xì)胞癌,大細(xì)胞癌明顯低于癌旁組織;3.Survivin和Klotho的表達(dá)與胃腸道神經(jīng)內(nèi)分泌癌患者組織分化、腫瘤分期相關(guān),兩者是預(yù)測和評估胃腸道神經(jīng)內(nèi)分泌癌患者預(yù)后的重要指標(biāo)。
[Abstract]:Objective: to detect the expression of Survivin and Klotho in cancer tissues and adjacent tissues of patients with gastrointestinal neuroendocrine carcinoma. To investigate the relationship between Survivin and Klotho and the prognosis of patients with gastrointestinal neuroendocrine carcinoma. Methods: the data of 51 patients with gastrointestinal neuroendocrine carcinoma were retrospectively studied. Immunohistochemical and Western blot methods were used to detect the expression of Survivin and Klotho in cancer tissues and adjacent tissues of GNT patients. The relationship between the expression of two proteins and the prognosis of patients with gastrointestinal neuroendocrine carcinoma was analyzed and studied. Results: Survivin was positive in 35 out of 51 patients with gastrointestinal neuroendocrine carcinoma. No expression of Survivin was found in the paracancerous tissues. The expression of Survivi in GNT and adjacent tissues was detected by immunohistochemical Blot. The expression of Survivin in small cell carcinoma was higher than that in large cell. The expression intensity and quantity of Survivin in small cell carcinoma were higher than that in large cell. There was no difference between the expression of Survivin in paracancerous tissues. Clinical data showed that the expression of Survivin was related to tumor stage and tissue differentiation. With age, Analysis showed that the expression of Survivin in poorly differentiated and undifferentiated cancer tissues was significantly higher than that in well-differentiated carcinoma tissues. The positive expression rate of Survivin in clinical stage 鈪，

本文編號：1543446