本文關(guān)鍵詞： FOXQ1 上皮間質(zhì)轉(zhuǎn)化(EMT) 大腸癌 轉(zhuǎn)移　出處：《重慶醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討FOXQ1 (Forkhead Box Q1,FOXQ1)基因?qū)Υ竽c癌細(xì)胞的上皮-間質(zhì)轉(zhuǎn)化(Epithelial-Mesenchymal transition, EMT)的作用及其可能的機(jī)制。方法：構(gòu)建針對人類FOXQ1基因的shRNA慢病毒沉默載體,轉(zhuǎn)染大腸癌細(xì)胞株SW480細(xì)胞,并篩選出沉默效果最好的干擾序列。后續(xù)實(shí)驗(yàn)設(shè)干擾組(感染FOXQ1-shl序列)、陰性組(感染NC-shRNA序列)、對照組。顯微鏡觀察三組細(xì)胞間形態(tài)學(xué)差異；Transwell小室、細(xì)胞黏附試驗(yàn)觀察三組細(xì)胞轉(zhuǎn)移能力及黏附能力的差異；qRT-PCR、W estern blot技術(shù)檢測E-cadherin、N-cadherin、Vimentin、MMP2基因和蛋白水平的差異。結(jié)果：FOXQ1-sh1慢病毒載體成功沉默了SW480細(xì)胞的FOXQ1的表達(dá)。干擾組與陰性組及對照組相比,細(xì)胞頂?shù)讟O性及細(xì)胞間緊密連接增加；侵襲、遷移的細(xì)胞數(shù)目顯著減少(P0.05)；同種黏附能力增加,異種黏附能力降低(P0.05)；qRT-PCR、Western blot顯示E-cadhe rin表達(dá)顯著增高而N-cadherin、Vimentin、MMP2表達(dá)顯著降低(P0.05)。結(jié)論：沉默F(xiàn)OXQ1基因能夠促進(jìn)大腸癌SW480細(xì)胞EMT的逆轉(zhuǎn),降低大腸癌SW480細(xì)胞的轉(zhuǎn)移能力,其機(jī)制可能與E-cadherin的上調(diào)和N-cadherin、Vimentin、MMP2的下調(diào)有關(guān)。
[Abstract]:Objective: To investigate the FOXQ1 (Forkhead Box Q1, FOXQ1) - gene on epithelial mesenchymal transition in human colon cancer cells (Epithelial-Mesenchymal, transition, EMT) function and its possible mechanism. Methods: to construct the shRNA lentiviral vector targeting human FOXQ1 gene silencing, transfected into colon cancer cell line SW480, and filter out the interference sequence silence effect the best. The subsequent experiment set interference group (infected with FOXQ1-shl sequence), negative group (control group, NC-shRNA infection sequence). To observe the morphological differences between the three groups of cells microscope; Transwell cell, cell adhesion test differences were observed in the three groups cell metastasis and adhesion; qRT-PCR, E-cadherin W estern blot detection, N-cadherin technology. Vimentin, MMP2 gene and protein level. Results: FOXQ1-sh1 lentiviral vector was successfully silenced the expression of SW480 FOXQ1 cells. The interference group and the negative group and control group Compared to the top and bottom cell tight junctions between cells increased; the number of invasion, cell migration was significantly reduced (P0.05); with the increasing of adhesion ability, reduce the heterogeneous adhesion ability (P0.05); qRT-PCR Western, blot E-cadhe showed that Rin expression was significantly increased while N-cadherin, Vimentin, MMP2 expression decreased significantly (P0.05). Conclusion: the silence FOXQ1 gene can promote human colorectal cancer cell line SW480 EMT reversal, reduce the metastatic ability of human colorectal cancer cell line SW480 and its possible mechanism of Vimentin and the upregulation of E-cadherin and N-cadherin, and down-regulation of MMP2.

【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R735.34

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本文編號：1538798