本文關(guān)鍵詞： 脫氧紫色桿菌素 HT細(xì)胞 凋亡　出處：《中國(guó)比較醫(yī)學(xué)雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的探討脫氧紫色桿菌素(Deoxyviolacein)對(duì)人結(jié)腸癌HT29細(xì)胞增殖抑制和誘導(dǎo)凋亡及其可能機(jī)制。方法用不同濃度的脫氧紫色桿菌素處理HT29細(xì)胞,采用四甲基偶氮唑藍(lán)(MTT)比色法,檢測(cè)脫氧紫色桿菌素對(duì)HT29細(xì)胞的抑制率;流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期和凋亡;Western Blot法檢測(cè)凋亡相關(guān)蛋白Bax、Bcl-2和Caspase9表達(dá)的變化。結(jié)果脫氧紫色桿菌素對(duì)HT29細(xì)胞有明顯的增殖抑制作用;可提高G0/G1期細(xì)胞比例,降低S期細(xì)胞比例;細(xì)胞凋亡率升高,Bax和Caspase9蛋白表達(dá)升高,Bcl-2蛋白的表達(dá)降低。結(jié)論脫氧紫色桿菌素抑制結(jié)腸癌HT29細(xì)胞增殖并誘導(dǎo)凋亡,其凋亡機(jī)制可能作用于線粒體介導(dǎo)的內(nèi)源性通路。
[Abstract]:Objective to investigate the inhibitory effect of deoxyviolacein on the proliferation and apoptosis of human colon cancer HT29 cells and its possible mechanism. Methods HT29 cells were treated with different concentrations of deoxyviolacein. The inhibitory rate of deoxypurple bactein on HT29 cells, the cell cycle by flow cytometry and the expression of apoptosis-related protein BaxanBcl-2 and Caspase9 were detected by flow cytometry. Results Deoxypurple bactein could inhibit the proliferation of HT29 cells. It can increase the proportion of G _ 0 / G _ 1 phase cells and decrease S phase cell ratio, increase the expression of Bax and Caspase9 protein and decrease the expression of Bcl-2 protein. Conclusion Deoxypurple bactein can inhibit the proliferation of colon cancer HT29 cells and induce apoptosis. The mechanism of apoptosis may play an important role in mitochondrial mediated endogenous pathway.
【作者單位】： 揚(yáng)州大學(xué)食品科學(xué)與工程學(xué)院;東南大學(xué)公共衛(wèi)生學(xué)院營(yíng)養(yǎng)與食品衛(wèi)生系教育部環(huán)境醫(yī)學(xué)工程重點(diǎn)實(shí)驗(yàn)室;清華大學(xué)化學(xué)工程系工業(yè)生物催化教育部重點(diǎn)實(shí)驗(yàn)室;中國(guó)肉類食品綜合研究中心;
【分類號(hào)】：R735.3
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本文編號(hào)：1519471